Anti-Np95 (Mouse) mAb

  • Applications
    • FCM
    • ICC
    • IHC
    • IP
    • WB
  • Target NP95
  • Host Species Rat
  • Species Reactivities Mouse
  • Code # D289-3
  • Size 100 μl
  • Price
    $328.61
Specifications

Alternative Names

ICBP90, RNF106, AL022808

Background

DNA methylation inheritance is the process of copying pre-existing methylation patterns onto newly replicated DNA strand after DNA replication. Dnmt1 is the maintenance DNA methyltransferase, which methylates hemimethylated CpGs, appears after DNA repairs and replications. The mouse Np95, also known as Uhrf1, is a nuclear protein, which contains an ubiquitin-like domain, tandem Tudor domains, a PHD (Plant Homeodomain) finger, an SRA (SET-and RING-associated) domain, and a RING (Really Interesting New Gene) finger domain. Np95 recognizes hemimethylated CpGs via the SRA domain, and recruits Dnmt1 to the sites to transfer the methylation patterns onto the newly synthesized DNA strand. Therefore, the interaction between Np95 and Dnmt1 is necessary for the inheritance of DNA methylation.
  • Antibody Type:
    Monoclonal
  • Application:
    FCM, ICC, IHC, IP, WB
  • Clone Number:
    Th-10a
  • Concentration:
    1 mg/mL
  • Conjugate:
    Unlabeled
  • Description:
    Monoclonal antibody of 100 µl targeting NP95 for FCM, ICC, IHC, IPP, WB.
  • Formulation:
    100 μg IgG in 100 μl volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.
  • Host Species:
    Rat
  • Immunogen:
    Mouse thymic lymphoma
  • Isotype:
    IgG2a ĸ
  • Product Type:
    Antibody
  • Reactivity:
    This antibody reacts with endogenous mouse Np95 (95 kDa) .
  • Research Area:
    Cell Biology
  • Short Description:
    NP95 Monoclonal Antibody.
  • Size:
    100 μl
  • Species Reactivity:
    Mouse
  • Storage Temperature:
    -20°C
  • Target:
    NP95
Citations
  1. Bonapace IM et al. Np95 is regulated by E1A during mitotic reactivation of terminally differentiated cells and is essential for S phase entry. J Cell Biol. 157, 909-14 (2002),
  2. Fujimori A et al. Cloning and mapping of Np95 gene which encodes a novel nuclear protein associated with cell proliferation. Mamm Genome. 9, 1032-5 (1998),
  3. Muto M et al. Targeted disruption of Np95 gene renders murine embryonic stem cells hypersensitive to DNA damaging agents and DNA replication blocks. J Biol Chem. 277, 34549-55 (2002),
  4. Muto M et al. The characterization of the monoclonal antibody Th-10a, specific for a nuclear protein appearing in the S phase of the cell cycle in normal thymocytes and its unregulated expression in lymphoma cell lines. Cell Prolif. 28, 645-57 (1995),
  5. Ohno R et al. A replication-dependent passive mechanism modulates DNA demethylation in mouse primordial germ cells. Development 140, 2892-903 (2013)
     ,
  6. Sharif J et al. The SRA protein Np95 mediates epigenetic inheritance by recruiting Dnmt1 to methylated DNA. Nature. 450, 908-12 (2007),
  7. Uemura T et al. Temporal and spatial localization of novel nuclear protein NP95 in mitotic and meiotic cells. Cell Struct Funct. 25, 149-59 (2000)
References
  1. Ohno, R., et al., Development 140, 2892-2903 (2013)
  2. Sharif, J., et al., Nature 450, 908-912 (2007) [IC]
  3. Muto, M., et al., Radiat. Res. 166, 723-733 (2006)
  4. Muto, M., et al., J. Biol. Chem. 277, 34549-34555 (2002)[WB]
  5. Bonapace, I. M., et al., J. Cell Biol. 157, 909-914 (2002) [WB, IC]
  6. Uemura, T., et al., Cell Struct. Funct. 25, 149-159 (2000) [IC]
  7. Fujimori, A., et al., Mamm. Genome 9, 1032-1035 (1998) [WB]
  8. Muto, M., et al., Cell Prolif. 28, 645-657 (1995)