Anti-CD63 (LAMP-3) (Mouse) mAb

  • Applications
    • FCM
    • WB
  • Target CD63
  • Host Species Rat
  • Species Reactivities Mouse
  • Code # D263-3
  • Size 100 μl
  • Price
    $261.78
Specifications

Background

CD63 is not only expressed on activated platelets, but also activated monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and translocated to cell surface upon various stimuli. The membrane of lytic granules in CTLs contains CD63/LAMP-3 and other lysosomal-associated glycoproteins (LAMPs) such as CD107a/LAMP-1 and CD107b/LAMP-2. LAMPs have been observed on the cell surface as a result of degranulation. CD63 belongs to a member of the tetraspanin transmembrane-protein (TM4) superfamily, which includes CD9, CD37, CD53, CD81, CD82, CD151 and CD231. Several members of this family form noncovalent associations with integrins, particularly β1 integrins (CD29), and modulate cellular adhesion properties. CD63 has a tyrosine-based internalization motif in the cytoplasmic C-terminal tail and interacts with adaptor protein complexes such as AP-2 and AP-3. Because AP-2 and AP-3 are involved in facilitating the clathrin-mediated endocytosis, CD63 could be directly involved in the internalization of its membrane protein partners.
  • Antibody Type:
    Monoclonal
  • Application:
    FCM, WB
  • Clone Number:
    R5G2
  • Concentration:
    1 mg/mL
  • Conjugate:
    Unlabeled
  • Description:
    Monoclonal antibody of 100 µl targeting CD63 for FCM, WB.
  • Formulation:
    100 μg IgG in 100 μl volume of PBS containing 50% glycerol, pH 7.2. No preservative iscontained.
  • Gene ID (Human):
  • Gene ID (Mouse):
  • Host Species:
    Rat
  • Immunogen:
    Mouse bone marrow stromal cell line ST2
  • Isotype:
    IgG2b
  • Product Type:
    Antibody
  • Reactivity:
    This antibody reacts with mouse CD63 on Western blotting and Flow cytometry.
  • Research Area:
    Immunology
  • Short Description:
    CD63 Monoclonal Antibody.
  • Size:
    100 μl
  • Species Reactivity:
    Mouse
  • Storage Temperature:
    -20°C
  • Target:
    CD63
Citations
  1. Zonneveld MI et al. Recovery of extracellular vesicles from human breast milk is influenced by sample collection and vesicle isolation procedures. J Extracell Vesicles. 3, 24215 (2014),

  2. Bobrie A et al. Diverse subpopulations of vesicles secreted by different intracellular mechanisms are present in exosome preparations obtained by differential ultracentrifugation. J Extracell Vesicles. 1, 18397 (2012),
  3. Kunert S et al. The microtubule modulator RanBP10 plays a critical role in regulation of platelet discoid shape and degranulation. Blood 114, 5532-40 (2009),
  4. Lopes da Silva M et al. The host endocytic pathway is essential for Plasmodium berghei late liver stage development. Traffic. 13, 1351-63 (2012),
  5. Päll T et al. Soluble CD44 interacts with intermediate filament protein vimentin on endothelial cell surface. PLoS One 6, e29305 (2011),
  6. Seto S et al. Differential recruitment of CD63 and Rab7-interacting-lysosomal-protein to phagosomes containing Mycobacterium tuberculosis in macrophages. Microbiol Immunol. 54,170-4 (2010),
  7. Ushio H et al. Crucial role for autophagy in degranulation of mast cells. J Allergy Clin Immunol. 127, 1267-76 (2011),
  8. Verjan Garcia N et al. SIRPα/CD172a regulates eosinophil homeostasis. J Immunol. 187, 2268-77 (2011)
References
  1. Liu, Y., et al., J. Exp. Med. 204, 93-103 (2007)
  2. Kabu, K., et al., J. Immunol. 177, 1296-1305 (2006)
  3. Nishida, K., et al., J. Cell Biol. 170, 115-126 (2005)
  4. Mitsunari, T., et al., Mol. Cell Biol. 25, 9318-9323 (2005)