Anti-MFG-E8 (Mouse) mAb

  • Applications
    • IHC
    • WB
  • Target MFG-E8
  • Host Species Hamster
  • Species Reactivities Mouse
  • Code # D199-3
  • Size 100 μg
  • Price
    $284.06
Specifications

Background

Apoptotic cells are rapidly removed by phagocytosis to clear the living cells and tissues of intracellular materials being released from dying cells. Phagocytes recognize apoptotic cells through milk fat globule-EGF-factor 8 (MFG-E8). MFG-E8 is a secreted glycoprotein from thioglycollate-elicited macrophages and bind to phosphatidylserine exposed on the cell surface of apoptotic cells, then bind strongly to cells expressing αvβ3 integrin via its RGD (arginine-glycine-aspartate) motif. Thus, MFG-E8 is an important protein to mediate the engulfment of apoptotic cells by activated macrophages.
  • Antibody Type:
    Monoclonal
  • Application:
    IHC, WB
  • Clone Number:
    18A2-G10
  • Concentration:
    1 mg/mL
  • Conjugate:
    Unlabeled
  • Description:

    Monoclonal antibody of 100 μg targeting MFG-E8 for IHC, WB.

  • Formulation:
    100 μg IgG in 100 μl volume of PBS containing 50% glycerol, pH 7.2. Contains nopreservatives.
  • Gene ID (Human):
  • Gene ID (Mouse):
  • Host Species:
    Hamster
  • Immunogen:
    Recombinant mouse MFG-E8
  • Isotype:
    IgG
  • Product Type:
    Antibody
  • Reactivity:
    This antibody reacts with mouse MFG-E8 short and long forms on Western blotting and Immunohistochemistry.
  • Research Area:
    Apoptosis
  • Short Description:

    MFG-E8 Monoclonal Antibody.

  • Size:
    100 μg
  • Species Reactivity:
    Mouse
  • Storage Temperature:
    -20°C
  • Target:
    MFG-E8
Citations
  1. Abe T et al. Regulation of osteoclast homeostasis and inflammatory bone loss by MFG-E8. J Immunol. 193, 1383-91 (2014),
  2. Aziz M et al. Milk fat globule-epidermal growth factor-factor 8 attenuates neutrophil infiltration in acute lung injury via modulation of CXCR2. J immunol. 189, 393-402 (2012),
  3. Aziz MM et al. Prolactin induces MFG-E8 production in macrophages via transcription factor C/EBPbeta-dependent pathway. Apoptosis. 13, 609-20 (2008),
  4. Bobrie A et al. Diverse subpopulations of vesicles secreted by different intracellular mechanisms are present in exosome preparations obtained by differential ultracentrifugation. J Extracell Vesicles. 1, 18397 (2012),
  5. Hoffhines AJ et al. Tyrosylprotein sulfotransferase-2 expression is required for sulfation of RNase 9 and Mfge8 in vivo. J Biol Chem. 284, 3096-105 (2009),
  6. Jinushi M et al. MFG-E8-mediated uptake of apoptotic cells by APCs links the pro- and antiinflammatory activities of GM-CSF. J Clin Invest. 117, 1902-13 (2007),
  7. Kranich J et al. Follicular dendritic cells control engulfment of apoptotic bodies by secreting Mfge8. J Exp Med. 205, 1293-302 (2008),
  8. Middendorp S et al. Mice deficient for CD137 ligand are predisposed to develop germinal center-derived B-cell lymphoma. Blood. 114, 2280-9 (2009),
  9. Yoshino M et al. Analysis of capturing skin antigens in the steady state using milk fat globule EGF factor 8-deficient skin-hyperpigmented mice. Immunol Lett. 115, 131-37 (2008)
References
  1. Middendorp, S., et al., Blood. 114, 2280-9 (2009) [IHC]
  2. Kranich, J., et al., J. Exp. Med. 205, 1293-1302 (2008) [IHC]
  3. Aziz, M. M. et al., Apoptosis. 13, 609-20 (2008) [WB]
  4. Yoshino, M. et al., Immunol. Lett. 115, 131-137 (2008) [IHC]
  5. Jinushi, M., et al., J.Clin.Invest. 117, 1902-1913 (2007) [IHC]
  6. Hanayama, R., et al., PNAS 102, 16886-16891 (2005)
  7. Hanayama, R., et al., Science 304, 1147-1150 (2004)
  8. Miyasaka, K., et al., Eur. J. Immunol. 34, 1414-1422 (2004)
  9. Hanayama, R., et al., J. Immunol. 172, 3876-3882 (2004)
  10. Hanayama, R., et al., Nature 417, 182-187 (2002)