Anti-MFG-E8 (Mouse) mAb (Monoclonal Antibody)

  • Applications
    • FCM
    • IP
  • Target MFG-E8
  • Host Species Hamster
  • Species Reactivities Mouse
  • Code # D161-3
  • Size 100 μg
  • Price
    $284.06
Specifications

Background

​Apoptotic cells are rapidly removed by phagocytosis to clear the living cells and tissues of intracellular materials being released from dying cells. Phagocytes recognize apoptotic cells through milk fat globule-EGF-factor 8 (MFG-E8). MFG-E8 is a secreted glycoprotein from thioglycollate-elicited macrophages and bind to phosphatidylserine exposed on the cell surface of apoptotic cells, then bind strongly to cells expressing ανβ3 integrin via its RGD (arginine-glycine-aspartate) motif. Thus, MFG-E8 is an important protein to mediate the engulfment of apoptotic cells by activated macrophages.
  • Antibody Type:
    Monoclonal
  • Application:
    FCM, IP
  • Clone Number:
    2422
  • Concentration:
    1 mg/mL
  • Conjugate:
    Unlabeled
  • Description:

    Monoclonal antibody of 100 μg targeting MFG-E8 for FCM, IPP.

  • Formulation:
    100 μg IgG in 100 μl volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.
  • Gene ID (Human):
  • Gene ID (Mouse):
  • Host Species:
    Hamster
  • Immunogen:
    Mouse peritoneal macrophages were attracted by Thioglycollate
  • Isotype:
    IgG
  • Product Type:
    Antibody
  • Reactivity:
    This antibody reacts with mouse MFG-E8 short and long form on Flow cytometry and Immunoprecipitation.
  • Research Area:
    Apoptosis
  • Short Description:

    MFG-E8 Monoclonal Antibody.

  • Size:
    100 μg
  • Species Reactivity:
    Mouse
  • Storage Temperature:
    -20°C
  • Target:
    MFG-E8
Citations
  1. Cui T et al. Milk fat globule epidermal growth factor 8 attenuates acute lung injury in mice after intestinal ischemia and reperfusion. Am J Respir Crit Care Med. 181, 238-46 (2010),
  2. Fens MH et al. Angiogenic endothelium shows lactadherin-dependent phagocytosis of aged erythrocytes and apoptotic cells. Blood 111, 4542-50 (2008),
  3. Hoffhines AJ et al. Tyrosylprotein sulfotransferase-2 expression is required for sulfation of RNase 9 and Mfge8 in vivo. J Biol Chem. 284, 3096-105 (2009),
  4. Miksa M et al. Fractalkine-induced MFG-E8 leads to enhanced apoptotic cell clearance by macrophages. Mol Med. 13, 553-60 (2007),
  5. Soki FN et al. Polarization of prostate cancer-associated macrophages is induced by milk fat globule-EGF factor 8 (MFG-E8)-mediated efferocytosis. J Biol Chem. 289, 24560-72 (2014),
  6. Usui K et al. Isolation and characterization of naïve follicular dendritic cells. Mol Immunol. 50, 172-6 (2012),
  7. Zhang F et al. Milk fat globule epidermal growth factor-factor 8 mitigates inflammation and tissue injury after hemorrhagic shock in experimental animals. J Trauma Acute Care Surg. 72, 861-9 (2012)
References
  1. Fens, M. H. A. M., et al., Blood 111, 4542-4550 (2008)
  2. Montecalvo, A., et al., J. Immunol. 180, 3081-3090 (2008)
  3. Hanayama, R., et al., Science 304, 1147-1150 (2004)
  4. Morelli, A. E., et al., Blood 104, 3257-3266 (2004)
  5. Hanayama, R., et al. J. Immunol. 172, 3876-3882 (2004)
  6. Hanayama, R., et al. Nature 417, 182-187 (2002)