Anti-GFP (Green Fluorescent Protein) mAb-Agarose (Monoclonal Antibody)

GFP Monoclonal Antibody.

Specifications:

Description

Monoclonal antibody of 10 µl targeting GFP for ICC, IPP conjugated to agarose.

Target: GFP
Product Type: Antibody
Size: 200 μg
Application: IP
Research Area / Disease: Epitope Tag
Conjugate: Agarose
Antibody Type: Monoclonal
Clone Number: RQ2
Formulation: 100 mg of anti-GFP monoclonal antibody covalently coupled to 200 mL of agarose gel and provided as a 50% gel slurry suspended in PBS containing preservative (0.09% sodium azide) for a total volume of 400 mL.
Isotype: IgG2a
Immunogen: GFP was affinity-purified anti-GFP by using a mouse from 293T cells expressing GFP
Host Species: Rat
Source: This antibody was purified from hybridoma (clone RQ2) supernatant using protein G agarose. This hybridoma was established by fusion of mouse myeloma cell PAI with Wister rat lymphnode immunized with GFP purified from GFP expressed 293T cells by affinity c
Reactivity: This antibody reacts with GFP fusion protein on Immunoprecipitation. It reacts with EBFP, ECFP, EGFP, Venus and Sapphire.
Storage Temperature: 4°C
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.

Citations

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  3. Celesnik H et al. Arabidopsis thaliana VOZ (Vascular plant One-Zinc finger) transcription factors are required for proper regulation of flowering time. Biol Open. 2,  424-31 (2013),
  4. Choi HI et al. Peroxiredoxin V selectively regulates IL-6 production by modulating the Jak2-Stat5 pathway. Free Radic Biol Med. 65C, 270-9 (2013),
  5. Darzacq X et al. Stepwise RNP assembly at the site of H/ACA RNA transcription in human cells. J Cell Biol. 173, 207-18 (2006),
  6. Fukumoto Y et al. Role of scaffold protein afadin dilute domain-interacting protein (ADIP) in platelet-derived growth factor-induced cell movement by activating Rac protein through Vav2 protein. J Biol Chem. 286, 43537-48 (2011),
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  8. He M et al. Systematic Analysis of the Functions of Lysine Acetylation in the Regulation of Tat Activity. PLoS One 8, e67186 (2013),
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  10. Heyman J et al. Arabidopsis ULTRAVIOLET-B-INSENSITIVE4 maintains cell division activity by temporal inhibition of the anaphase-promoting complex/cyclosome. Plant Cell 23, 4394-410 (2011),
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  13. Roberts D et al. Modulation of phototropic responsiveness in Arabidopsis through ubiquitination of phototropin 1 by the CUL3-Ring E3 ubiquitin ligase CRL3(NPH3). Plant Cell 23, 3627-40 (2011),
  14. Sasabe M et al. Phosphorylation of a mitotic kinesin-like protein and a MAPKKK by cyclin-dependent kinases (CDKs) is involved in the transition to cytokinesis in plants. PNAS 108,17844-9 (2011),
  15. Shirae-Kurabayashi M et al. Ci-Pem-1 localizes to the nucleus and represses somatic gene transcription in the germline of Ciona intestinalis embryos. Development 138, 2871-81 (2011),
  16. Tanaka T et al. Drosophila Mon2 couples Oskar-induced endocytosis with actin remodeling for cortical anchorage of the germ plasm. Development 138, 2523-32 (2011),
  17. Yen YT et al. Differential localization of the streptococcal accessory sec components and implications for substrate export. J Bacteriol. 195, 682-95 (2013),
  18. Zhong Y et al. Importin beta interacts with the endoplasmic reticulum-associated degradation machinery and promotes ubiquitination and degradation of mutant alpha1-antitrypsin. J Biol Chem. 286, 33921-30 (2011)
    1. Tomida J et al. FAM35A associates with REV7 and modulates DNA damage responses of normal and BRCA1-defective cells. EMBO J. 37, e99543 (2018)
    2. Yu X et al. The chromatin remodeler ZmCHB101 impacts expression of osmotic stress-responsive genes in maize. Plant Mol Biol. 97, 451-465 (2018)
    3. Yu X et al. The chromatin remodeler ZmCHB101 impacts alternative splicing contexts in response to osmotic stress. Plant Cell Rep. 38 131-145 (2019)
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    References

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    2. Sato, Y., et al., J. Biol. Chem. 284, 11873-11881 (2009)
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    4. Kato, A., et al., J. Virol. 82, 6172-6189 (2008)
    5. Dragone, L. L., et al. PNAS. 103, 18202-18207 (2006)
    6. Darzacq, X., et al. J. Cell Biol. 173, 207-218 (2006)
    7. Hayakawa, T., et al. Plant Cell Physiol. 47, 891-904 (2006)
    8. Obuse, C., et al. Nat. Cell Biol. 6, 1135-1141 (2004)
    Code D153-8
    Product Type Antibody
    Size 200 μg
    Application IP
    Price
    $328.61
    Qty: