Anti-GFP (Green Fluorescent Protein) mAb-Agarose (Monoclonal Antibody)
GFP Monoclonal Antibody.


Specifications:
Description
Monoclonal antibody of 10 µl targeting GFP for ICC, IPP conjugated to agarose.
Target: | GFP |
---|---|
Product Type: | Antibody |
Size: | 200 μg |
Application: | IP |
Research Area / Disease: | Epitope Tag |
Conjugate: | Agarose |
Antibody Type: | Monoclonal |
Clone Number: | RQ2 |
Formulation: | 100 mg of anti-GFP monoclonal antibody covalently coupled to 200 mL of agarose gel and provided as a 50% gel slurry suspended in PBS containing preservative (0.09% sodium azide) for a total volume of 400 mL. |
Isotype: | IgG2a |
Immunogen: | GFP was affinity-purified anti-GFP by using a mouse from 293T cells expressing GFP |
Host Species: | Rat |
Source: | This antibody was purified from hybridoma (clone RQ2) supernatant using protein G agarose. This hybridoma was established by fusion of mouse myeloma cell PAI with Wister rat lymphnode immunized with GFP purified from GFP expressed 293T cells by affinity c |
Reactivity: | This antibody reacts with GFP fusion protein on Immunoprecipitation. It reacts with EBFP, ECFP, EGFP, Venus and Sapphire. |
Storage Temperature: | 4°C |
Regulatory Statement: | For Research Use Only. Not for use in diagnostic procedures. |
Citations
- Aung K, Hu J. The Arabidopsis tail-anchored protein PEROXISOMAL AND MITOCHONDRIAL DIVISION FACTOR1 is involved in the morphogenesis and proliferation of peroxisomes and mitochondria. Plant Cell 23, 4446-61 (2011),
- Cai L et al. Regulation of fertility, survival, and cuticle collagen function by the Caenorhabditis elegans eaf-1 and ell-1 genes. J Biol Chem. 286, 35915-21 (2011),
- Celesnik H et al. Arabidopsis thaliana VOZ (Vascular plant One-Zinc finger) transcription factors are required for proper regulation of flowering time. Biol Open. 2, 424-31 (2013),
- Choi HI et al. Peroxiredoxin V selectively regulates IL-6 production by modulating the Jak2-Stat5 pathway. Free Radic Biol Med. 65C, 270-9 (2013),
- Darzacq X et al. Stepwise RNP assembly at the site of H/ACA RNA transcription in human cells. J Cell Biol. 173, 207-18 (2006),
- Fukumoto Y et al. Role of scaffold protein afadin dilute domain-interacting protein (ADIP) in platelet-derived growth factor-induced cell movement by activating Rac protein through Vav2 protein. J Biol Chem. 286, 43537-48 (2011),
- Hayakawa T et al. ACT domain repeat protein 7, ACR7, interacts with a chaperone HSP18.0-CII in rice nuclei. Plant Cell Physiol. 47, 891-904 (2006),
- He M et al. Systematic Analysis of the Functions of Lysine Acetylation in the Regulation of Tat Activity. PLoS One 8, e67186 (2013),
- Henderson MJ et al. Mesencephalic astrocyte-derived neurotrophic factor (MANF) secretion and cell surface binding are modulated by KDEL receptors. J Biol Chem. 288, 4209-25 (2013),
- Heyman J et al. Arabidopsis ULTRAVIOLET-B-INSENSITIVE4 maintains cell division activity by temporal inhibition of the anaphase-promoting complex/cyclosome. Plant Cell 23, 4394-410 (2011),
- Liu Q et al. Complementation of Hyponastic Leaves1 by Double-strand RNA Binding Domains of Dicer-like 1 in Nuclear Dicing Bodies. Plant Physiol. 163, 108-17 (2013),
- Paster W et al. GRB2-mediated recruitment of THEMIS to LAT is essential for thymocyte development. J Immunol.190, 3749-56 (2013),
- Roberts D et al. Modulation of phototropic responsiveness in Arabidopsis through ubiquitination of phototropin 1 by the CUL3-Ring E3 ubiquitin ligase CRL3(NPH3). Plant Cell 23, 3627-40 (2011),
- Sasabe M et al. Phosphorylation of a mitotic kinesin-like protein and a MAPKKK by cyclin-dependent kinases (CDKs) is involved in the transition to cytokinesis in plants. PNAS 108,17844-9 (2011),
- Shirae-Kurabayashi M et al. Ci-Pem-1 localizes to the nucleus and represses somatic gene transcription in the germline of Ciona intestinalis embryos. Development 138, 2871-81 (2011),
- Tanaka T et al. Drosophila Mon2 couples Oskar-induced endocytosis with actin remodeling for cortical anchorage of the germ plasm. Development 138, 2523-32 (2011),
- Yen YT et al. Differential localization of the streptococcal accessory sec components and implications for substrate export. J Bacteriol. 195, 682-95 (2013),
- Zhong Y et al. Importin beta interacts with the endoplasmic reticulum-associated degradation machinery and promotes ubiquitination and degradation of mutant alpha1-antitrypsin. J Biol Chem. 286, 33921-30 (2011)
- Tomida J et al. FAM35A associates with REV7 and modulates DNA damage responses of normal and BRCA1-defective cells. EMBO J. 37, e99543 (2018)
- Yu X et al. The chromatin remodeler ZmCHB101 impacts expression of osmotic stress-responsive genes in maize. Plant Mol Biol. 97, 451-465 (2018)
- Yu X et al. The chromatin remodeler ZmCHB101 impacts alternative splicing contexts in response to osmotic stress. Plant Cell Rep. 38 131-145 (2019)
- Xu Z et al. O-GlcNAcylation regulates integrin-mediated cell adhesion and migration via formation of focal adhesion complexes. J Biol Chem. 294, 3117-3124 (2019)
References
- Cai, L., et al., J. Biol. Chem. 286, 35915-35921 (2011)
- Sato, Y., et al., J. Biol. Chem. 284, 11873-11881 (2009)
- Sakurai, T., et al., J. Cell Biol. 183, 339-352 (2008)
- Kato, A., et al., J. Virol. 82, 6172-6189 (2008)
- Dragone, L. L., et al. PNAS. 103, 18202-18207 (2006)
- Darzacq, X., et al. J. Cell Biol. 173, 207-218 (2006)
- Hayakawa, T., et al. Plant Cell Physiol. 47, 891-904 (2006)
- Obuse, C., et al. Nat. Cell Biol. 6, 1135-1141 (2004)