Anti-GFP (Green Fluorescent Protein) mAb (Monoclonal Antibody)
GFP Monoclonal Antibody.
Specifications:
Description
Monoclonal antibody of 100 µl targeting GFP for ICC, IPP.
| Target: | GFP |
|---|---|
| Product Type: | Antibody |
| Size: | 100 µl |
| Application: | ICC, IP |
| Research Area / Disease: | Epitope Tag |
| Conjugate: | Unlabeled |
| Antibody Type: | Monoclonal |
| Clone Number: | RQ2 |
| Concentration: | 1 mg/mL |
| Formulation: | 100 μg IgG in 100 μl volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained. |
| Isotype: | IgG2a |
| Immunogen: | GFP was affinity-purified anti-GFP by using a mouse from 293T cells expressing GFP |
| Host Species: | Rat |
| Source: | This antibody was purified from hybridoma (clone RQ2) supernatant using protein G agarose. This hybridoma was established by fusion of mouse myeloma cell PAI with Wister rat lymphonode immunized with GFP purified from GFP expressed 293T cells by affinity |
| Reactivity: | This antibody reacts with GFP fusion protein on Immunoprecipitation and Immunocytochemistry. It reacts with EBFP, ECFP, EGFP, Venus and Sapphire |
| Storage Temperature: | -20°C |
| Regulatory Statement: | For Research Use Only. Not for use in diagnostic procedures. |
Citations
- Obuse C et al. A conserved Mis12 centromere complex is linked to heterochromatic HP1 and outer kinetochore protein Zwint-1. Nat Cell Biol. 6, 1135-41 (2004),
- Qi Q et al. Involvement of the N-terminal B-box domain of Arabidopsis BBX32 protein in interaction with soybean BBX62 protein. J Biol Chem. 287, 31482-93 (2012),
- Sakurai T et al. Membrane microdomain switching: a regulatory mechanism of amyloid precursor protein processing. J Cell Biol. 183, 339-52 (2008),
- Sugiyama T et al. Red5 and three nuclear pore components are essential for efficient suppression of specific mRNAs during vegetative growth of fission yeast. Nucleic Acids Res. 41, 6674-86 (2013)
- Urata Y et al. Spatio-temporal neural stem cell behavior leads to both perfect and imperfect structural brain regeneration in adult newts. Biol Open 7, bio033142 (2018)
- Li M et al. p53 cooperates with SIRT6 to regulate cardiolipin de novo biosynthesis. Cell Death Dis. 9, 941 (2018)
- Hatakeyama H et al. Cooperative actions of Tbc1d1 and AS160/Tbc1d4 in GLUT4-trafficking activities. J Biol Chem. 294, 1161-1172 (2019)rn
References
- Sugiyama, T., et al.,Nucleic Acids Res.41,6674-6686(2013) [IP]
- Qi, Q., et al., J. Biol. Chem. 287, 31482-31493 (2012) [Co-IP]
- Cai, L., et al., J. Biol. Chem. 286, 35915-35921 (2011)
- Sato, Y., et al., J. Biol. Chem. 284, 11873-11881 (2009)
- Sakurai, T., et al., J. Cell Biol. 183, 339-352 (2008) [IP]
- Kato, A., et al., J. Virol. 82, 6172-6189 (2008)
- Dragone, L. L., et al. PNAS. 103, 18202-18207 (2006)
- Darzacq, X., et al. J. Cell Biol. 173, 207-218 (2006)
- Hayakawa, T., et al. Plant Cell Physiol. 47, 891-904 (2006)
- Obuse, C., et al. Nat. Cell Biol. 6, 1135-1141 (2004) [IP]
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