Anti-GFP (Green Fluorescent Protein) mAb (Monoclonal Antibody)

GFP Monoclonal Antibody.



Monoclonal antibody of 100 µl targeting GFP for ICC, IPP.

Target: GFP
Product Type: Antibody
Size: 100 µl
Application: ICC, IP
Research Area / Disease: Epitope Tag
Conjugate: Unlabeled
Antibody Type: Monoclonal
Clone Number: RQ2
Concentration: 1 mg/mL
Formulation: 100 μg IgG in 100 μl volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.
Isotype: IgG2a
Immunogen: GFP was affinity-purified anti-GFP by using a mouse from 293T cells expressing GFP
Host Species: Rat
Source: This antibody was purified from hybridoma (clone RQ2) supernatant using protein G agarose. This hybridoma was established by fusion of mouse myeloma cell PAI with Wister rat lymphonode immunized with GFP purified from GFP expressed 293T cells by affinity
Reactivity: This antibody reacts with GFP fusion protein on Immunoprecipitation and Immunocytochemistry. It reacts with EBFP, ECFP, EGFP, Venus and Sapphire
Storage Temperature: '-20°C
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.


  1. Obuse C et al. A conserved Mis12 centromere complex is linked to heterochromatic HP1 and outer kinetochore protein Zwint-1. Nat Cell Biol. 6, 1135-41 (2004),
  2. Qi Q et al. Involvement of the N-terminal B-box domain of Arabidopsis BBX32 protein in interaction with soybean BBX62 protein. J Biol Chem. 287, 31482-93 (2012),
  3. Sakurai T et al. Membrane microdomain switching: a regulatory mechanism of amyloid precursor protein processing. J Cell Biol. 183, 339-52 (2008),
  4. Sugiyama T et al. Red5 and three nuclear pore components are essential for efficient suppression of specific mRNAs during vegetative growth of fission yeast. Nucleic Acids Res. 41, 6674-86 (2013)
  5. Urata Y et al. Spatio-temporal neural stem cell behavior leads to both perfect and imperfect structural brain regeneration in adult newts. Biol Open 7, bio033142 (2018)
  6. Li M et al. p53 cooperates with SIRT6 to regulate cardiolipin de novo biosynthesis. Cell Death Dis. 9, 941 (2018)
  7. Hatakeyama H et al. Cooperative actions of Tbc1d1 and AS160/Tbc1d4 in GLUT4-trafficking activities. J Biol Chem. 294, 1161-1172 (2019)rn


  1. Sugiyama, T., et al.,Nucleic Acids Res.41,6674-6686(2013) [IP]
  2. Qi, Q., et al., J. Biol. Chem. 287, 31482-31493 (2012) [Co-IP]
  3. Cai, L., et al., J. Biol. Chem. 286, 35915-35921 (2011)
  4. Sato, Y., et al., J. Biol. Chem. 284, 11873-11881 (2009)
  5. Sakurai, T., et al., J. Cell Biol. 183, 339-352 (2008) [IP]
  6. Kato, A., et al., J. Virol. 82, 6172-6189 (2008)
  7. Dragone, L. L., et al. PNAS. 103, 18202-18207 (2006)
  8. Darzacq, X., et al. J. Cell Biol. 173, 207-218 (2006)
  9. Hayakawa, T., et al. Plant Cell Physiol. 47, 891-904 (2006)
  10. Obuse, C., et al. Nat. Cell Biol. 6, 1135-1141 (2004) [IP]
Code D153-3
Product Type Antibody
Size 100 µl
Application ICC, IP