Anti-GFP (Green Fluorescent Protein) mAb

  • Applications
    • ICC
    • IP
  • Target GFP
  • Host Species Rat
  • Code # D153-3
  • Size 100 μl
  • Price
    $306.33
Specifications

Background

Since the detection of intracellular Aequorea Victria Green Fluorescent Protein (GFP) requires only irradiation by UV or blue light, it provides an excellent means for monitoring gene expression and protein localization in living cells. Monoclonal anti-GFP antibody can detect GFP fusion protein on Immunoprecipitation.
  • Antibody Type:
    Monoclonal
  • Application:
    ICC, IP
  • Clone Number:
    RQ2
  • Concentration:
    1 mg/mL
  • Conjugate:
    Unlabeled
  • Description:

    Monoclonal antibody of 100 µl targeting GFP for ICC, IPP.

  • Formulation:
    100 μg IgG in 100 μl volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.
  • Host Species:
    Rat
  • Immunogen:
    GFP was affinity-purified anti-GFP by using a mouse from 293T cells expressing GFP
  • Isotype:
    IgG2a
  • Product Type:
    Antibody
  • Reactivity:
    This antibody reacts with GFP fusion protein on Immunoprecipitation and Immunocytochemistry. It reacts with EBFP, ECFP, EGFP, Venus and Sapphire
  • Research Area:
    Epitope Tag
  • Short Description:

    GFP Monoclonal Antibody.

  • Size:
    100 μl
  • Storage Temperature:
    -20°C
  • Target:
    GFP
Citations
  1. Obuse C et al. A conserved Mis12 centromere complex is linked to heterochromatic HP1 and outer kinetochore protein Zwint-1. Nat Cell Biol. 6, 1135-41 (2004),
  2. Qi Q et al. Involvement of the N-terminal B-box domain of Arabidopsis BBX32 protein in interaction with soybean BBX62 protein. J Biol Chem. 287, 31482-93 (2012),
  3. Sakurai T et al. Membrane microdomain switching: a regulatory mechanism of amyloid precursor protein processing. J Cell Biol. 183, 339-52 (2008),
  4. Sugiyama T et al. Red5 and three nuclear pore components are essential for efficient suppression of specific mRNAs during vegetative growth of fission yeast. Nucleic Acids Res. 41, 6674-86 (2013)
References
  1. Sugiyama, T., et al.,Nucleic Acids Res.41,6674-6686(2013) [IP]
  2. Qi, Q., et al., J. Biol. Chem. 287, 31482-31493 (2012) [Co-IP]
  3. Cai, L., et al., J. Biol. Chem. 286, 35915-35921 (2011)
  4. Sato, Y., et al., J. Biol. Chem. 284, 11873-11881 (2009)
  5. Sakurai, T., et al., J. Cell Biol. 183, 339-352 (2008) [IP]
  6. Kato, A., et al., J. Virol. 82, 6172-6189 (2008)
  7. Dragone, L. L., et al. PNAS. 103, 18202-18207 (2006)
  8. Darzacq, X., et al. J. Cell Biol. 173, 207-218 (2006)
  9. Hayakawa, T., et al. Plant Cell Physiol. 47, 891-904 (2006)
  10. Obuse, C., et al. Nat. Cell Biol. 6, 1135-1141 (2004) [IP]