Anti-PIP3 (Phosphatidylinositol-3, 4, 5-triphosphate) mAb

  • Applications
    • DB
  • Target PIP3
  • Host Species Mouse
  • Code # D145-3
  • Size 100 μl
  • Price
    $334.18
Specifications

Background

Phosphoinositide (PI) 3-kinases phosphorylate inositol lipids at the 3' position of the inositol ring to generate the 3-phosphoinositides, including PtdIns(3)P, PtdIns(3,4)P2 and PtdIns(3,4,5)P3. These phosphoinositides function as key second messengers in signal transduction and membrane trafficking. PIP3 plays multiple roles in regulating cell growth and survival. Approximately 50% of all cancers possess mutations in genes that regulate PIP3 production, resulting in cells with abnormally high levels of PIP3. PIP3 acts as a critical second messenger in insulin signaling, where it interacts with the Pleckstrin homology domains of PKB and PDK1, co-localizing these enzymes at the plasma membrane and allowing PDK1 to activate PKB. PIP3 is a more potent PKC-activator than PIP2, activating even Ca2+-insensitive PKC isotypes δ, ε, and η. PIP3 is also involved in T cell activation via CD28, regulating exocytosis, and inducing actin reorganization and membrane ruffling.
  • Antibody Type:
    Monoclonal
  • Application:
    DB
  • Clone Number:
    NN111.1.1
  • Conjugate:
    Unlabeled
  • Description:

    Monoclonal antibody of 100 µl targeting PIP3 for Dot Blot.

  • Formulation:
    100 μg IgG in 100 μl volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.
  • Host Species:
    Mouse
  • Immunogen:
    Purified PtdIns (3,4) P2
  • Isotype:
    IgG1 ĸ
  • Product Type:
    Antibody
  • Reactivity:
    This antibody reacts with PtdIns(3,4,5) P3, and also faintly reacts with PtdIns(3,4) P.
  • Research Area:
    Cell Biology
  • Short Description:

    PIP3 Monoclonal Antibody.

  • Size:
    100 μl
  • Storage Temperature:
    -20°C
  • Target:
    PIP3
References
  1. Takabayashi, T., et al., J. Biol. Chem. 285, 16155-16165 (2010)
  2. Nishio, Y., et al., Anal. Biochem. 285, 270-273 (2000)