Anti-Ring1B (Mouse) mAb

  • Applications
    • IP
    • WB
  • Target Ring1b
  • Host Species Mouse
  • Species Reactivities Hamster, Human, Mouse
  • Code # D139-3
  • Size 100 μg
  • Price
    $350.89
Specifications

Background

Polycomb-group (PcG) proteins form multimeric complexes that maintain the state of transcriptional repression of several regulatory genes during development. Ring1B/Rnf2 forms part of a protein complex containing other PcG proteins, such as Mel18, Bmi1, MBLR, MPc3, and the spliceosome protein Sap155, and these complexes associate with chromatin to regulate transcription. Ring1B may also play a role in the regulation of Hox gene expression by PcG complexes. Deletion of Ring1B activity results in gastrulation arrest and cell cycle inhibition.
  • Antibody Type:
    Monoclonal
  • Application:
    IP, WB
  • Clone Number:
    42432
  • Concentration:
    1 mg/mL
  • Conjugate:
    Unlabeled
  • Description:
    Monoclonal antibody of 100 μg targeting Ring1b for IPP, WB, CHiP.
  • Formulation:
    100 μg IgG in 100 μl volume of PBS containing 50% glycerol, pH 7.2. No preservative iscontained.
  • Gene ID (Human):
  • Gene ID (Mouse):
  • Host Species:
    Mouse
  • Immunogen:
    Recombinant mouse full-length Ring1B
  • Isotype:
    IgG2b
  • Product Type:
    Antibody
  • Reactivity:
    This antibody reacts with human, mouse, and hamster Ring1B.
  • Research Area:
    Cancer
  • Short Description:
    Ring1b Monoclonal Antibody.
  • Size:
    100 μg
  • Species Reactivity:
    Hamster, Human, Mouse
  • Storage Temperature:
    -20°C
  • Target:
    Ring1b
Citations
  1. Alchanati I et al. The E3 ubiquitin-ligase Bmi1/Ring1A controls the proteasomal degradation of Top2alpha cleavage complex - a potentially new drug target. PLoS One 4, e8104 (2009),
  2. Barco R et al. The synovial sarcoma-associated SYT-SSX2 oncogene antagonizes the polycomb complex protein Bmi1. PLoS One 4, e5060 (2009),
  3. Casanova M et al. Heterochromatin reorganization during early mouse development requires a single-stranded noncoding transcript. Cell Rep. 4, 1156-67 (2013),
  4. Landeira D et al. Jarid2 is a PRC2 component in embryonic stem cells required for multi-lineage differentiation and recruitment of PRC1 and RNA Polymerase II to developmental regulators. Nat Cell Biol. 12, 618-24 (2010),
  5. Mendenhall EM et al. GC-rich sequence elements recruit PRC2 in mammalian ES cells. PLoS Genet. 6, e1001244 (2010),
  6. Pradeepa MM et al. Psip1/Ledgf p75 restrains Hox gene expression by recruiting both trithorax and polycomb group proteins. Nucleic Acids Res. 42, 9021-32 (2014),
  7. Sarcinella E et al. Monoubiquitylation of H2A.Z distinguishes its association with euchromatin or facultative heterochromatin. Mol Cell Biol. 27, 6457-68 (2007),
  8. Vallot C et al. PRC2-independent chromatin compaction and transcriptional repression in cancer. Oncogene 34, 741-51 (2015),
  9. Williamson I et al. Anterior-posterior differences in HoxD chromatin topology in limb development. Development 139, 3157-67 (2012),
  10. Wu J et al. Histone ubiquitination associates with BRCA1-dependent DNA damage response. Mol Cell Biol. 29, 849-60 (2009),
  11. Wu X et al. Cooperation between EZH2, NSPc1-mediated histone H2A ubiquitination and Dnmt1 in HOX gene silencing. Nucleic Acid Res. 36, 3590-9 (2008),
  12. Zaaroor-Regev D et al. Regulation of the polycomb protein Ring1B by self-ubiquitination or by E6-AP may have implications to the pathogenesis of Angelman syndrome. PNAS 107, 6788-93 (2010)
References
  1. Atsuta, T., et al. Hybridoma 20, 43-46 (2001)
  2. Suzuki, M., et al. Development 129, 4171-4183 (2002)