Anti-ASC (TMS1) (Human) mAb (Monoclonal Antibody)
ASC Monoclonal Antibody.
Specifications:
Description
Monoclonal antibody of 100 μg targeting ASC for ICC, IHC, IPP, WB.
| Target: | ASC |
|---|---|
| Product Type: | Antibody |
| Size: | 100 μg |
| Application: | ICC, IHC, IP, WB |
| Research Area / Disease: | Apoptosis |
| Conjugate: | Unlabeled |
| Antibody Type: | Monoclonal |
| Clone Number: | 23-4 |
| Formulation: | 100 μg IgG in a 100 μl volume of PBS containing 50% glycerol, pH 7.2. Contains nopreservatices. |
| Isotype: | IgG1 |
| Immunogen: | TritonX-100 insoluble fraction of HL-60 cells were treated with retinoic acid |
| Host Species: | Mouse |
| Species Reactivity: | Human |
| Source: | This antibody was purified from hybridoma (clone 23-4) supernatant using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell P3-X63-AG8.653 with Balb/c mouse splenocyte immunized with Triton X-100 insoluble fraction of retino |
| Reactivity: | This antibody reacts with human ASC (22 kDa) on Western blotting and Immunoprecipitation. |
| Gene ID Human: | |
| Gene ID Mouse: | |
| Storage Temperature: | '-20°C |
| Regulatory Statement: | For Research Use Only. Not for use in diagnostic procedures. |
Citations
- Bryan NB et al. Differential splicing of the apoptosis-associated speck like protein containing a caspase recruitment domain (ASC) regulates inflammasomes. J Inflamm (Lond). 7, 23 (2010),
- Masumoto J et al. ASC, a novel 22-kDa protein, aggregates during apoptosis of human promyelocytic leukemia HL-60 cells. J Biol Chem. 274, 33835-8 (1999),
- Masumoto J et al. Expression of apoptosis-associated speck-like protein containing a caspase recruitment domain, a pyrin N-terminal homology domain-containing protein, in normal human tissues. J Histochem Cytochem. 49, 1269-75 (2001),
- Masumoto J et al. Murine ortholog of ASC, a CARD-containing protein, self-associates and exhibits restricted distribution in developing mouse embryos. Exp Cell Res. 262, 128-33 (2001),
- Masumoto J et al. Pyrin N-terminal homology domain- and caspase recruitment domain-dependent oligomerization of ASC. Biochem Biophys Res Commun. 280, 652-5 (2001),
- Mayor A et al. A crucial function of SGT1 and HSP90 in inflammasome activity links mammalian and plant innate immune responses. Nat Immunol. 8, 497-503. (2007),
- Terasawa K et al. Epigenetic inactivation of TMS1/ASC in ovarian cancer. Clin Cancer Res. 10, 2000-6 (2004)
References
- Bryan, N. B., et al., J. Inflamm. (Lond.) 7, 23 (2010)
- Mayor, A., et al., Nat. Immunol. 8, 497-503 (2007)
- Hasegawa, M., et al., J. Biol. Chem. 280, 15122-15130 (2005)
- Terasawa, K., et al., Clin. Cancer Res. 10, 2000-2006 (2004)
- Wang, Y., et al., Int. Immunol. 16, 777-786 (2004)
- Stehlik, C., et al., J. Immunol. 171, 6154-6163 (2003)
- Stehlik, C., et al., J. Exp. Med. 196, 1605-1615 (2002)
- Masumoto, J., et al., J. Histochem. Cytochem. 49, 1269-1275 (2001)
- Masumoto, J., et al., Exp. Cell Res. 262, 128-133 (2001)
- Masumoto, J., et al., Biochem. Biophys. Res. Commun. 280, 652-655 (2001)
- Masumoto, J., et al., J. Biol. Chem. 274, 33835-33838 (1999)
