Anti-ST2 (Human) mAb-PE

  • Applications
    • FCM
    • IP
    • WB
  • Target ST2
  • Host Species Mouse
  • Species Reactivities Human
  • Code # D067-5
  • Size 1 mL (50 Tests)
  • Price
    $306.33
Specifications

Alternative Names

T1, Fit1, DER4

Background

The ST2 gene, also known as T1, Fit1, or DER4, was originally identified as a responsive gene that was highly induced by stimulation of various proliferation-inducing agents including serum, PDGF (platelet-derived growth factor), FGF (fibroblast growth factor), or lysophosphatidic acid in murine fibroblasts. Three distinct forms of gene products have been reported and named ST2, ST2V, and ST2L. ST2 is a soluble secreted form of 37 kDa protein, which lacks intracellular domain, whereas ST2L is a transmembrane form of 62 kDa protein (the glycosylated forms of ST2 and ST2L are about 57 and 80 kDa, respectively). This ST2L protein is very similar to IL-1R (interleukin-1 receptor) type I and II in structure, thus it is considered as a member of the IL-1R family. ST2V, which is another novel variant form of human ST2, has been identified recently. ST2 proteins are expressed in the wide variety types of human cells, including hematopoietic cells in various stages of differentiation, a population of the peripheral blood mononuclear cells from healthy individuals, glioblastoma and astrocytoma cell lines, and colon cancer cells in addition to fibroblast cell lines. Thus ST2 proteins are considered to have some roles in regulating cell growth or proliferation. On the other hand, either definitive functions of ST2 proteins or their ligand molecule(s) which binds to ST2 proteins have remained unclear, though it has been reported that none of IL-1α, β, RA (receptor antagonist) binds to ST2 proteins in spite of their structural similarity to IL-1R. This indicates that ST2L protein is functionally independent from IL-1R. Furthermore, several studies have shown that ST2L is expressed on the cell surface of Th2 cells but not on the Th1 cells, indicating the possibility that ST2L protein participates not only in the regulation of cell growth or proliferation, but also in the immune system including differentiation of T cells or immunological response via helper T cells. From these observations, ST2 proteins are considered to be one of the important proteins participate in various physiological phenomenon, thus further analysis are required to understand its physiological functions.
  • Antibody Type:
    Monoclonal
  • Application:
    FCM, IP, WB
  • Clone Number:
    2A5
  • Conjugate:
    PE
  • Description:
    Monoclonal antibody conjugated to PE of 50 tests targeting ST2 for FCM, IPP, WB.
  • Formulation:
    50 tests in 1 mL volume of PBS containing 1% BSA and 0.09% NaN3.*Azide may react with copper or lead in plumbing system to formexplosive metal azides. Therefore, always flush plenty of waterwhen disposing materials containing azide into drain.
  • Gene ID (Human):
  • Gene ID (Mouse):
  • Host Species:
    Mouse
  • Immunogen:
    ST2 secreted form was purified from the culture supernatant of COS7 cell transfectants
  • Isotype:
    IgG1
  • Product Type:
    Antibody
  • Reactivity:
    This antibody reacts with human ST2 on Flow cytometry.
  • Research Area:
    Cancer
  • Short Description:
    ST2 Monoclonal Antibody.
  • Size:
    1 mL (50 Tests)
  • Species Reactivity:
    Human
  • Storage Temperature:
    4°C
  • Target:
    ST2
Citations
  1. Allakhverdi Z et al. Cutting edge: The ST2 ligand IL-33 potently activates and drives maturation of human mast cells. J Immunol. 179, 2051-4 (2007),
  2. Zeyda M et al. Severe obesity increases adipose tissue expression of interleukin-33 and its receptor ST2, both predominantly detectable in endothelial cells of human adipose tissue. Int J Obes (Lond). 37, 658-65 (2013)
References
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  8. Yanagisawa, K., et al., J. Biochem. 121, 95-103 (1997)
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  10. Lanahan, A., et al., Mol. Cell. Biol.12, 3919-3929 (1992)
  11. Tominaga, S., et al., FEBS Lett. 258, 301-304 (1989)