Products Product Type Antibodies Monoclonal Anti-Multi Ubiquitin mAb (Monoclonal Antibody)

Anti-Multi Ubiquitin mAb (Monoclonal Antibody)

Ubiquitin Monoclonal Antibody.

Data Sheet

Specifications:

Description

Monoclonal antibody of 100 μg targeting Ubiquitin for WB.

Target: Ubiquitin
Product Type: Antibody
Size: 100 μg
Application: WB
Research Area / Disease: Cell Biology
Conjugate: Unlabeled
Antibody Type: Monoclonal
Clone Number: FK2
Concentration: 1 mg/mL
Formulation: 100 μg IgG in 100 μl volume of PBS containing 50% glycerol, pH 7.2. No preservative iscontained.
Isotype: IgG1
Immunogen: Lysozyme - poly-ubiquitin was purified crude
Host Species: Mouse
Species Reactivity: Human
Source: This antibody was purified from hybridoma(clone FK2) supernatant using protein A agarose. Thishybridoma was established by fusion of mouse myelomacell with Balb/c mouse splenocyte immunized with a crudepoly-ubiquitin-lysozyme.
Reactivity: This antibody reacts with multiubiquitin chains, but it doesn’t react with mono ubiquitinand free ubiquitin on Western blotting.
Storage Temperature: -20°C
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.

Citations

  1. Broering TJ et al. Carboxyl-proximal regions of reovirus nonstructural protein muNS necessary and sufficient for forming factory-like inclusions. J Virol. 79, 6194-6206 (2005),
  2. Choi UY et al. Polyubiquitin chain-dependent protein degradation in TRIM30 cytoplasmic bodies. Exp. Mol.Med. 47, e159 (2015),
  3. Ebina M et al. Myeloma overexpressed 2 (Myeov2) regulates L11 subnuclear localization through Nedd8 modification. PLoS One. 8, e65285 (2013),
  4. Furuya N et al. PARK2/Parkin-mediated mitochondrial clearance contributes to proteasome activation during slow-twitch muscle atrophy via NFE2L1 nuclear translocation. Autophagy 10, 631-41 (2014),
  5. Gitcho MA et al. VCP mutations causing frontotemporal lobar degeneration disrupt localization of TDP-43 and induce cell death. J Biol Chem. 284, 12384-98 (2009),
  6. Hosokawa H et al. Regulation of Th2 cell development by Polycomb group gene bmi-1 through the stabilization of GATA3. J Immunol. 177, 7656-64 (2006),
  7. Hwang GW et al. Overexpression of Rad23 confers resistance to methylmercury in saccharomyces cerevisiae via inhibition of the degradation of ubiquitinated proteins. Mol Pharmacol. 68, 1074-8 (2005),
  8. Inukai N et al. A novel hydrogen peroxide-induced phosphorylation and ubiquitination pathway leading to RNA polymerase II proteolysis. J Biol Chem. 279, 8190-5 (2004),
  9. Ishioka T et al. Impairment of the ubiquitin-proteasome system by cellular FLIP. Genes Cells 12, 735-44 (2007),
  10. Katoh K et al. The ALG-2-interacting protein Alix associates with CHMP4b, a human homologue of yeast Snf7 that is involved in multivesicular body sorting. J Biol Chem. 278, 39104-13 (2003),
  11. Komatsu M et al. Homeostatic levels of p62 control cytoplasmic inclusion body formation in autophagy-deficient mice. Cell 131, 1149-1163 (2007),
  12. Masuda Y et al. ADRP/adipophilin is degraded through the proteasome-dependent pathway during regression of lipid-storing cells. J Lipid Res. 47, 87-98 (2006),
  13. Nakamura M et al. Clathrin anchors deubiquitinating enzymes, AMSH and AMSH-like protein, on early endosomes. Genes Cells 11, 593-606 (2006),
  14. Seino H et al. Two ubiquitin-conjugating enzymes, UbcP1/Ubc4 and UbcP4/Ubc11, have distinct functions for ubiquitination of mitotic cyclin. Mol Cell Biol. 23, 3497-3505 (2003),
  15. Shi W et al. Disassembly of MDC1 foci is controlled by ubiquitin-proteasome-dependent degradation. J Biol Chem. 283, 31608-16 (2008),
  16. Yamashita M et al. Ras-ERK MAPK cascade regulates GATA3 stability and Th2 differentiation through ubiquitin-proteasome pathway. J Biol Chem. 280, 29409-19 (2005)
  17. Matsumoto G et al. N-Acyldopamine induces aggresome formation without proteasome inhibition and enhances protein aggregation via p62/SQSTM1 expression. Sci Rep. 8, 9585 (2018)
  18. Jena KK et al. TRIM16 controls assembly and degradation of protein aggregates by modulating the p62‐NRF2 axis and autophagy. EMBO J. 37, e98358 (2018)
  19. Chernyshova K et al. Glaucoma-Associated Mutations in the Optineurin Gene Have Limited Impact on Parkin-Dependent Mitophagy. Invest Ophthalmol Vis Sci. 60, 3625-3635 (2019)

References

  1. Ishioka, T., et al., Genes Cells 12, 735-744 (2007)
  2. Hosokawa, H., et al., J. Immunol. 177, 7656-7664 (2006)
  3. Masuda, Y., et al., J. Lipid Res. 47, 87-98 (2006)
  4. Nakamura, M., et al., Genes Cells 11, 593-606 (2006)
  5. Yamashita, M., et al., J. Biol. Chem. 280, 29409-29419 (2005)
  6. Hwang, G-W., et al., Mol. Pharmacol. 68, 1074-1078 (2005)
  7. Broering, T. J., et al., J. Virol. 79, 6194-6206 (2005)
  8. Inukai, N., et al., J. Biol. Chem. 279, 8190-8195 (2004)
  9. Katoh, K., et al., J. Biol. Chem. 278, 39104-39113 (2003)
  10. Seino, H., et al., Mol. Cell Biol. 23, 3497-3505 (2003)
  11. Yokosawa, N., et al., J. Virol. 76, 12683-12690 (2002)
  12. Takada, K., et al., Eur. J. Biochem. 233, 42-47 (1995)
  13. Fujimuro, M., et al., FEBS Lett. 349, 173-180 (1994)
Code D058-3
Product Type Antibody
Size 100 μg
Application WB
Price
$382.88
Qty:
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