Anti-CD43 (Human) mAb-PE

  • Applications
    • FCM
  • Target CD43
  • Host Species Mouse
  • Species Reactivities Human
  • Code # D056-5
  • Size 50 Tests
  • Price
    $306.33
Specifications

Background

CD43 antigen is a heavily sialylated single chain membrane glycoprotein with both molecular weight and antigenicity depending on the nature and complexity of the attached O-linked polysaccharides. CD43 is an integral membrane protein with extracellular domain (235 aa), transmembrane domain (23 aa) and intracytoplasmic domain (123 aa). The extracellular domain of CD43 has been shown to react with many ligand such as ICAM-1 (CD54), E-selectin, galectin-1, MHC-I and human serum albumin (HSA). The intracytoplasmic domain, which is highly conserved between species, is constitutively phosphorylated on serine residues. The level of phosphorylation increases after cell activation. This cell surface glycoprotein is also called leukosialin, sialophorin or GPL115. A soluble form of CD43 is also present in human serum. Thymocytes, CD4+ T lymphocytes and monocytes express more of a 115 kDa CD43 isoform, whereas a 130 kDa isoform is found mostly on activated CD4+ T cells, CD8+ resting and activated T cells, neutrophils, platelets and B cells. CD43 plays a role in the regulation of lymphoid cell adhesion, cellular maturation and activation although the nature of this participation remains controversial. For example, the structural characteristics of the CD43 extracellular domain indicate that this molecule has both adhesive and anti-adhesive properties. O-glycosylated chains of CD43 such as a sialyl Lewis X (sLex) epitope, a ligand for both P- and E-selectin, potentiate binding to lectin-like molecules. However, the adhesion molecule function for CD43 is counteracted by the presence of negatively charged sialic acid residues on its extracellular domain. In addition to the strong structural evidence for the involvement of CD43 in cell interactions, experimental data suggest contradictive role for CD43 in regulating cell contacts.
  • Antibody Type:
    Monoclonal
  • Application:
    FCM
  • Clone Number:
    1D4
  • Conjugate:
    PE
  • Description:
    Monoclonal antibody conjugated to PE of 50 tests targeting CD43 for FCM.
  • Formulation:
    50 tests in 1 mL volume of PBS containing 1% BSA and 0.09% NaN3.*Azide may react with copper orlead in plumbing system to form explosive metal azides. Therefore, always flush plenty ofwater when disposing materials containing azide into drain.
  • Gene ID (Human):
  • Gene ID (Mouse):
  • Host Species:
    Mouse
  • Immunogen:
    Human T cell line activated by PHA in medium containing IL-2
  • Isotype:
    IgG2a
  • Product Type:
    Antibody
  • Reactivity:
    This antibody reacts with CD43 antigen on Flow cytometry. Clone 1D4 selectively recognizes core 2-containing O-glycans on CD43, which have the GlcNAc1→6 branch attached to the C-6 position of GalNAc and are formed by core 2 -1,6-N-acetylglucosaminyltransf
  • Research Area:
    Cell Biology
  • Short Description:
    CD43 Monoclonal Antibody.
  • Size:
    50 Tests
  • Species Reactivity:
    Human
  • Storage Temperature:
    4°C
  • Target:
    CD43
Citations
  1. Weishaupt, C., et al., Clin. Cancer Res. 13, 2549-2556 (2007)
  2. Hernandez, J. D., et al., J. Immunol. 177, 5328-5336 (2006)
  3. Cabrera, P. V., et al., Blood 108, 2399-2406 (2006)
  4. Fuhlbrigge, R. C., et al., Blood 107, 1421-1426 (2006)
  5. Walcheck, B., et al., Blood 99, 4063-4069 (2002)
  6. Harrington, L. E., et al., J. Exp. Med. 191, 1241-1246 (2000)
  7. Mukasa, R., et al., Immunol. Lett. 67, 117-124 (1999)
  8. Mukasa, R., et al., Int. Immunol. 11, 259-268 (1999)