Anti-Sca-1 (Mouse) mAb-PE

  • Applications
    • FCM
  • Target Sca-1
  • Host Species Rat
  • Species Reactivities Mouse
  • Code # D052-5
  • Size 50 Tests
  • Price
    $261.78
Specifications

Background

Sca-1 is a member of the Ly-6 antigen family with molecular mass of 8 kDa under non-reducing conditions and 18 kDa under reducing conditions. Mouse hematopoietic stem cells express low levels of Thy-1 antigen (Thy-1lo) and to be negative for lineage markers (Lin-) that is, not expressing markers characteristic of B cells (B220), granulocytes (Gr-1), myelomonocytic cells (Mac-1) and T lymphocytes (CD4 and CD8). Recently, a new monoclonal antibody, anti-Sca-1, was used to purify stem cells from the Thy-1lo, Lin- bone marrow subpopulation. Thy-1lo, Lin-, Sca-1+ (but not the Thy-1lo, Lin-, Sca-1-) population of bone marrow cells are highly purified pluripotent stem cells. They read out with nearly unit efficiency in assays for primitive myeloerythroid and thymic progenitor, and have a capability to admit lethally irradiated mouse to survive and be restored in all blood-cell lineages. The Thy-1lo, Lin-, Sca-1+ subpopulation thought to have all stem cells present in the bone marrow.
  • Antibody Type:
    Monoclonal
  • Application:
    FCM
  • Clone Number:
    238B
  • Conjugate:
    PE
  • Description:
    Monoclonal antibody conjugated to PE of 50 tests targeting Sca-1 for FCM.
  • Formulation:
    50 tests in 1 mL volume of PBS containing 1% BSA and 0.09% NaN3.*Azide may react with copper orlead in plumbing system to form explosive metal azides. Therefore, always flush plenty ofwater when disposing materials containing azide into drain.
  • Gene ID (Human):
  • Gene ID (Mouse):
  • Host Species:
    Rat
  • Immunogen:
    LO cells were transfected with mouse Sca-1
  • Isotype:
    IgG2a ĸ
  • Product Type:
    Antibody
  • Reactivity:
    This antibody reacts with mouse Sca-1 on Flow cytometry.
  • Research Area:
    Cell Biology
  • Short Description:
    Sca-1 Monoclonal Antibody.
  • Size:
    50 Tests
  • Species Reactivity:
    Mouse
  • Storage Temperature:
    4°C
  • Target:
    Sca-1
References
  1. Petersen, B. E., et al., Hepatology 37, 632-640 (2003)