Anti-IL-18 (Clone 39-3F) (Mouse) mAb

  • Applications
    • NT
    • WB
  • Target IL-18
  • Host Species Rat
  • Species Reactivities Mouse
  • Code # D046-3
  • Size 100 μl
  • Price
    $306.33
Specifications

Alternative Names

interleukin 18 (interferon-γ-inducing factor)

Background

Interleukin 18 (IL - 18) is a n 18 kDa cytokine which identified as a c ostimulatory factor for production of interferon - γ (IFN - γ ) in response to toxic shock and shares functional similarities with IL - 12. IL - 18 is synthesized as a precursor 24 kDa molecule without a signal peptide and must be cleaved to produce an active molec ule. IL - 1 converting enzyme (ICE, Caspase - 1) cleaves pro - IL - 18 at aspartic acid in the P1 position, producing the mature, bioactive peptide that is readily released from the cells. It is reported that IL - 18 is produced from Kupffer cells, activated macroph ages, keratinocytes, intestinal epithelial cells, osteoblasts, adrenal cortex cells and murine diencephalon. IFN - γ is produced by activated T or NK cells and plays critical roles in the defense against microbiral pathogens. IFN - γ activates macrophages, enhances NK activity and B cell maturation, proliferation and Ig secretion, induces MHC class I and II antigens, and inhibits osteoclast activation. IL - 18 acts on T helper type - 1 (Th1) T cells and in combination with IL - 12 strongly induces them to produce IFN - γ . Pleiotropic effects of IL - 18 has also been reported, such as, enhancement production of IFN - γ and GM - CSF in p eripheral blood mononuclear cells, production of Th1 cytokines, IL - 2, GM - CSF and IFN - γ in T cells, enhancement of Fas ligand expression by Th1 cells.
  • Antibody Type:
    Monoclonal
  • Application:
    NT, WB
  • Clone Number:
    39-3F
  • Concentration:
    1 mg/mL
  • Conjugate:
    Unlabeled
  • Description:
    Monoclonal antibody of 100 µl targeting IL-18 for WB, NT.
  • Formulation:
    100 µg IgG in 100 µl volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.
  • Gene ID (Human):
  • Gene ID (Mouse):
  • Host Species:
    Rat
  • Immunogen:
    Recombinant mouse IL-18
  • Isotype:
    IgG2a
  • Product Type:
    Antibody
  • Reactivity:
    This antibody reacts with mouse IL-18on Western blotting.
  • Research Area:
    Immunology
  • Short Description:
    IL-18 Monoclonal Antibody.
  • Size:
    100 μl
  • Species Reactivity:
    Mouse
  • Storage Temperature:
    -20°C
  • Target:
    IL-18
Citations
  1. Hoshino T et al. Pulmonary inflammation and emphysema: role of the cytokines IL-18 and IL-13. Am J Respir Crit Care Med. 176, 49-62 (2007),
  2. Hoshino T et al. Cutting edge: IL-18-transgenic mice: in vivo evidence of a broad role for IL-18 in modulating immune function. J Immunol. 166, 7014-8 (2001),
  3. Lertmemongkolchai G et al. Bystander activation of CD8+ T cells contributes to the rapid production of IFN-gamma in response to bacterial pathogens. J. Immunol. 166, 1097-105 (2001),
  4. Seino H et al. IL-18 mediates the formation of stress-induced, histamine-dependent gastric lesions. Am J Physiol Gastrointest Liver Physiol. 292, G262-7 (2007),
  5. Venkatachalam K et al. Neutralization of interleukin-18 ameliorates ischemia/reperfusion-induced myocardial injury. J Biol Chem. 284,
    7853-65 (2009) 
  6. Venkatachalam, K., et al., J. Biol. Chem. 284, 7853-7865 (2009) [WB]
  7. Hoshino, T., et al., Am. J. Respir. Crit. Care Med. 176 49-62 (2007) [WB]
  8. Seino, H., et al., Am. J. Physiol. Gastrointest. Liver Physiol. 292, G262-G267 (2007) [WB]
  9. Hoshino, T., et al., J. Immunol. 166, 7014-7018 (2001) [WB]
  10. Lertmemongkolchai, G., et al., J. Immunol. 166, 1097-1105 (2001) [Neutralization]
  11. Hoshino T, Kato S, Oka N, et al. Pulmonary inflammation and emphysema: role of the cytokines IL-18 and IL-13. Am J Respir Crit Care Med. 2007;176(1):49-62.
References
  1. Dao, T., et al. Cell Immunol. 173, 230-235 (1996)
  2. Ushio, S., et al. J. Immunol. 156, 4274-4279 (1996)
  3. Micallef, M., et al. Eur. J. Immunol. 26, 1647-1651 (1996)
  4. Okamura, H., et al. Nature 378, 88-91 (1995)