Anti-IL-18 (Human) mAb-Biotin

  • Applications
    • ELISA
  • Target IL-18
  • Host Species Rat
  • Species Reactivities Human
  • Code # D045-6
  • Size 50 µl
  • Price
    $311.90
Specifications

Alternative Names

interleukin 18 (interferon-γ-inducing factor)

Background

Interleukin 18 (IL - 18) is a n 18 kDa cytokine which identified as a c ostimulatory factor for production of interferon - γ (IFN - γ ) in response to toxic shock and shares functional similarities with IL - 12. IL - 18 is synthesized as a precursor 24 kDa molecule without a signal peptide and must be cleaved to produce an active molec ule. IL - 1 converting enzyme (ICE, Caspase - 1) cleaves pro - IL - 18 at aspartic acid in the P1 position, producing the mature, bioactive peptide that is readily released from the cells. It is reported that IL - 18 is produced from Kupffer cells, activated macroph ages, keratinocytes, intestinal epithelial cells, osteoblasts, adrenal cortex cells and murine diencephalon. IFN - γ is produced by activated T or NK cells and plays critical roles in the defense against microbiral pathogens. IFN - γ activates macrophages, enhances NK activity and B cell maturation, proliferation and Ig secretion, induces MHC class I and II antigens, and inhibits osteoclast activation. IL - 18 acts on T helper type - 1 (Th1) T cells and in combination with IL - 12 strongly induces them to produce IFN - γ . Pleiotropic effects of IL - 18 has also been reported, such as, enhancement production of IFN - γ and GM - CSF in p eripheral blood mononuclear cells, production of Th1 cytokines, IL - 2, GM - CSF and IFN - γ in T cells, enhancement of Fas ligand expression by Th1 cells.
  • Antibody Type:
    Monoclonal
  • Application:
    ELISA
  • Clone Number:
    159-12B
  • Concentration:
    500 μg/ml
  • Conjugate:
    Biotin
  • Description:
    Monoclonal antibody of 50 µl targeting IL-18 for ELISA.
  • Formulation:
    25µg IgG in 50 µl volume of PBS containing 1% BSA and 0.09% NaN3
  • Gene ID (Human):
  • Gene ID (Mouse):
  • Host Species:
    Rat
  • Immunogen:
    Recombinant human IL-18
  • Isotype:
    IgG2a
  • Product Type:
    Antibody
  • Reactivity:
    This antibody reacts with human IL-18on ELISA.
  • Research Area:
    Immunology
  • Short Description:
    IL-18 Monoclonal Antibody.
  • Size:
    50 µl
  • Species Reactivity:
    Human
  • Storage Temperature:
    4°C
  • Target:
    IL-18
Citations
  1. Bontkes HJ et al. Tumor associated antigen and interleukin-12 mRNA transfected dendritic cells enhance effector function of natural killer cells and antigen specific T-cells. Clin Immunol. 127, 375-94 (2008),
  2. Giavedoni LD. Simultaneous detection of multiple cytokines and chemokines from nonhuman primates using luminex technology. J Immunol Methods. 301, 89-101 (2005),
  3. Leach ST et al. Local and systemic interleukin-18 and interleukin-18-binding protein in children with inflammatory bowel disease. Inflamm Bowel Dis.
    14, 68-74 (2008),
  4. Okano M et al. Characterization of IL-18 expression and release in the pathogenesis of chronic rhinosinusitis.  Int Arch Allergy Immunol. 160, 275-86 (2013)
References
  1. McCurnin, D., et al., Pediatrics 121, 945-956 (2008)
  2. Haveman, L. M., et al., Int. Immunol. 18, 1521-1529 (2006)
  3. Shigehara, K., et al., J. Immunol. 166, 642-649 (2001)
  4. Dao, T., et al. Cell Immunol. 173, 230-235 (1996)
  5. Micallef, M., et al. Eur. J. Immunol. 26, 1647-1651 (1996)
  6. Ushio, S., et al. J. Immunol. 156, 4274-4279 (1996)
  7. Okamura, H., et al. Nature 378, 88-91 (1995)