Anti-IL-18 (Clone 125-2H) (Human) mAb

  • Applications
    • ELISA
    • IP
    • NT
  • Target IL-18
  • Host Species Mouse
  • Species Reactivities Human
  • Code # D044-3
  • Size 100 μg
  • Price
    $328.61
Specifications

Alternative Names

interleukin 18 (interferon-γ-inducing factor)

Background

Interleukin 18 (IL - 18) is a n 18 kDa cytokine which identified as a c ostimulatory factor for production of interferon - γ (IFN - γ ) in response to toxic shock and shares functional similarities with IL - 12. IL - 18 is synthesized as a precursor 24 kDa molecule without a signal peptide and must be cleaved to produce an active molec ule. IL - 1 converting enzyme (ICE, Caspase - 1) cleaves pro - IL - 18 at aspartic acid in the P1 position, producing the mature, bioactive peptide that is readily released from the cells. It is reported that IL - 18 is produced from Kupffer cells, activated macroph ages, keratinocytes, intestinal epithelial cells, osteoblasts, adrenal cortex cells and murine diencephalon. IFN - γ is produced by activated T or NK cells and plays critical roles in the defense against microbiral pathogens. IFN - γ activates macrophages, enhances NK activity and B cell maturation, proliferation and Ig secretion, induces MHC class I and II antigens, and inhibits osteoclast activation. IL - 18 acts on T helper type - 1 (Th1) T cells and in combination with IL - 12 strongly induces them to produce IFN - γ . Pleiotropic effects of IL - 18 has also been reported, such as, enhancement production of IFN - γ and GM - CSF in p eripheral blood mononuclear cells, production of Th1 cytokines, IL - 2, GM - CSF and IFN - γ in T cells, enhancement of Fas ligand expression by Th1 cells.
  • Antibody Type:
    Monoclonal
  • Application:
    ELISA, IP, NT
  • Clone Number:
    125-2H
  • Concentration:
    1 mg/mL
  • Conjugate:
    Unlabeled
  • Description:
    Monoclonal antibody of 100 μg targeting IL-18 for ELISA, IPP, NT.
  • Formulation:
    100 µg IgG in 100 µl volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.
  • Gene ID (Human):
  • Gene ID (Mouse):
  • Host Species:
    Mouse
  • Immunogen:
    Recombinant human IL-18
  • Isotype:
    IgG1
  • Product Type:
    Antibody
  • Reactivity:
    This antibody reacts with human IL-18on Western blotting.
  • Research Area:
    Immunology
  • Short Description:
    IL-18 Monoclonal Antibody.
  • Size:
    100 μg
  • Species Reactivity:
    Human
  • Storage Temperature:
    -20°C
  • Target:
    IL-18
Citations
  1. Nussbaumer O et al. DC-like cell-dependent activation of human natural killer cells by the bisphosphonate zoledronic acid is regulated by γδ T lymphocytes. Blood. 118, 2743-51 (2011),
  2. Okano M et al. Characterization of IL-18 expression and release in the pathogenesis of chronic rhinosinusitis. Int Arch Allergy Immunol. 160, 275-86 (2013),
  3. Shida K et al. An alternative form of IL-18 in human blood plasma: complex formation with IgM defined by monoclonal antibodies. J Immunol. 166, 6671-9 (2001),
  4. Skurk T et al. The proatherogenic cytokine interleukin-18 is secreted by human adipocytes. Eur J Endocrinol. 152, 863-8 (2005),
  5. Sugawara S et al. Neutrophil proteinase 3-mediated induction of bioactive IL-18 secretion by human oral epithelial cells. J Immunol. 167, 6568-75 (2001),
  6. Tu Z et al. TLR-dependent cross talk between human Kupffer cells and NK cells. J Exp Med. 205, 233-44 (2008)
  7. Nussbaumer, O., et al., Blood 118, 2743-2751 (2011)
  8. Tu, A., et al., J. Exp. Med. 205, 233-244 (2008)
  9. Wu, C., J. Immunol. 170, 5571-5577 (2003)
  10. Sugawara, S., J. Immunol. 167, 6568-6575 (2001)
  11. Skurk T, Kolb H, Müller-scholze S, Röhrig K, Hauner H, Herder C. The proatherogenic cytokine interleukin-18 is secreted by human adipocytes. Eur J Endocrinol. 2005;152(6):863-8.
  12. Nagai T, Devergne O, Mueller TF, Perkins DL, Van seventer JM, Van seventer GA. Timing of IFN-beta exposure during human dendritic cell maturation and naive Th cell stimulation has contrasting effects on Th1 subset generation: a role for IFN-beta-mediated regulation of IL-12 family cytokines and IL-18 in naive Th cell differentiation. J Immunol. 2003;171(10):5233-43.
  13. Shida K, Shiratori I, Matsumoto M, et al. An alternative form of IL-18 in human blood plasma: complex formation with IgM defined by monoclonal antibodies. J Immunol. 2001;166(11):6671-9.
  14. Vankayalapati R, Wizel B, Weis SE, Samten B, Girard WM, Barnes PF. Production of interleukin-18 in human tuberculosis. J Infect Dis. 2000;182(1):234-9.
References
  1. Dao, T., et al., Cell Immunol. 173, 230-235 (1996)
  2. Micallef, M., et al., Eur. J. Immunol. 26, 1647-1651 (1996)
  3. Ushio, S., et al., J. Immunol. 156, 4274-4279 (1996)
  4. Okamura, H., et al., Nature 378, 88-91 (1995)