Anti-IL-18 (Clone 25-2G) (Human) mAb

  • Applications
    • IHC
    • WB
  • Target IL-18
  • Host Species Mouse
  • Species Reactivities Human
  • Code # D043-3
  • Size 100 μl
  • Price
    $306.33
Specifications

Alternative Names

interleukin 18 (interferon-γ-inducing factor)

Background

Interleukin 18 (IL - 18) is a n 18 kDa cytokine which identified as a c ostimulatory factor for production of interferon - γ (IFN - γ ) in response to toxic shock and shares functional similarities with IL - 12. IL - 18 is synthesized as a precursor 24 kDa molecule without a signal peptide and must be cleaved to produce an active molec ule. IL - 1 converting enzyme (ICE, Caspase - 1) cleaves pro - IL - 18 at aspartic acid in the P1 position, producing the mature, bioactive peptide that is readily released from the cells. It is reported that IL - 18 is produced from Kupffer cells, activated macroph ages, keratinocytes, intestinal epithelial cells, osteoblasts, adrenal cortex cells and murine diencephalon. IFN - γ is produced by activated T or NK cells and plays critical roles in the defense against microbiral pathogens. IFN - γ activates macrophages, enhances NK activity and B cell maturation, proliferation and Ig secretion, induces MHC class I and II antigens, and inhibits osteoclast activation. IL - 18 acts on T helper type - 1 (Th1) T cells and in combination with IL - 12 strongly induces them to produce IFN - γ . Pleiotropic effects of IL - 18 has also been reported, such as, enhancement production of IFN - γ and GM - CSF in p eripheral blood mononuclear cells, production of Th1 cytokines, IL - 2, GM - CSF and IFN - γ in T cells, enhancement of Fas ligand expression by Th1 cells.
  • Antibody Type:
    Monoclonal
  • Application:
    IHC, WB
  • Clone Number:
    25-2G
  • Concentration:
    1 mg/mL
  • Conjugate:
    Unlabeled
  • Description:
    Monoclonal antibody of 100 µl targeting IL-18 for IHC, WB.
  • Formulation:
    100 µg IgG in 100 µl volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.
  • Gene ID (Human):
  • Gene ID (Mouse):
  • Host Species:
    Mouse
  • Immunogen:
    Recombinant human IL-18
  • Isotype:
    IgG1
  • Product Type:
    Antibody
  • Reactivity:
    This antibody reacts with human IL-18on Western blotting.
  • Research Area:
    Immunology
  • Short Description:
    IL-18 Monoclonal Antibody.
  • Size:
    100 μl
  • Species Reactivity:
    Human
  • Storage Temperature:
    -20°C
  • Target:
    IL-18
Citations
  1. Sakai A, Sugawara Y, Kuroishi T, Sasano T, Sugawara S. Identification of IL-18 and Th17 cells in salivary glands of patients with Sjögren's syndrome, and amplification of IL-17-mediated secretion of inflammatory cytokines from salivary gland cells by IL-18. J Immunol. 2008;181(4):2898-906.
  2. Omoto Y, Tokime K, Yamanaka K, et al. Human mast cell chymase cleaves pro-IL-18 and generates a novel and biologically active IL-18 fragment. J Immunol. 2006;177(12):8315-9.
  3. Takiyama Y, Miyokawa N, Tokusashi Y, et al. Thyroid-stimulating hormone induces interleukin-18 gene expression in FRTL-5 cells: immunohistochemical detection of interleukin-18 in autoimmune thyroid disease. Thyroid. 2002;12(11):935-43.
  4. Shida K, Shiratori I, Matsumoto M, et al. An alternative form of IL-18 in human blood plasma: complex formation with IgM defined by monoclonal antibodies. J Immunol. 2001;166(11):6671-9.
References
  1. Sakai, A., et al., J. Immunol. 181, 2898-2906(2008)
  2. Takiyama, Y., et al., Thyroid 12, 935-943 (2002)
  3. Shida, K., et al., J. Immunol. 166, 6671-6679 (2001)
  4. Akita, K., et al., J. Biol. Chem. 272, 26595-26603 (1997)
  5. Dao, T., et al., Cell Immunol. 173, 230-235 (1996)
  6. Micallef, M., et al., Eur. J. Immunol. 26, 1647-1651 (1996)
  7. Ushio, S., et al., J. Immunol. 156, 4274-4279 (1996)
  8. Okamura, H., et al., Nature 378, 88-91 (1995)