Anti-Proton Pump (H, K-ATPase α subunit) (Mouse) mAb

  • Applications
    • IHC
    • IP
    • WB
  • Target Proton pump
  • Host Species Mouse
  • Species Reactivities Bovine, Human, Mouse, Rat
  • Code # D031-3
  • Size 100 μg
  • Price
    $306.33
Specifications

Background

Hydrogen-potassium adenosine triphosphatase (H + , K + -ATPase) belongs to a family of P-type cation-transporting ATPases that also includes Ca 2+ -ATPase and Na + , K + -ATPase. H + , K + -ATPase is responsible for acid secre tion into the stomach and catalyzes electroneutral excha nge of cytoplasmic hydrogen ions and external potassium ions coupled with ATP hydrolysis. The enzyme is found in gastric parietal cells where it is located in cytoplasmi c vesicles or apical plasma membranes of the secretory canaliculus. H + , K + -ATPase is a heterodimer consisting of a high molecular weight catalytic α subunit and a smaller but heavily glycosylated α subunit. The α subunit of H + , K + -ATPase has 10 transmembrane domains and contains sites for ATP binding and its acylphosphorylation, binding sites of proton pump inhibitors, and sites responsible for ion recognition.
  • Antibody Type:
    Monoclonal
  • Application:
    IHC, IP, WB
  • Clone Number:
    1H9
  • Concentration:
    1 mg/ml
  • Conjugate:
    Unlabeled
  • Description:
    Monoclonal antibody of 100 μg targeting Proton pump (H,K-ATPase α subunit) for IHC, IPP, WB.
  • Formulation:
    100 µg IgG in 100 µl volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.
  • Gene ID (Human):
  • Gene ID (Mouse):
  • Gene ID (Rat):
  • Host Species:
    Mouse
  • Immunogen:
    This product is an antibody obtained from a hybridoma by fusing mouse myeloma cells and spleen cells BALB / c nu / + mouse neonatal thymectomy anti-gastric wall cells to produce antibodies.
  • Isotype:
    IgG1
  • Product Type:
    Antibody
  • Reactivity:
    This antibody reacts with human, mouse, rat and bovine H + , K + -ATPase α subunit.
  • Research Area:
    Apoptosis
  • Short Description:
    Proton pump (H,K-ATPase α subunit) Monoclonal Antibody.
  • Size:
    100 μg
  • Species Reactivity:
    Bovine, Human, Mouse, Rat
  • Storage Temperature:
    -20°C
  • Target:
    Proton pump
Citations
  1. Becker V et al. New aspects in the pathomechanism and diagnosis of the laryngopharyngeal reflux-clinical impact of laryngeal proton pumps and pharyngeal pH metry in extraesophageal gastroesophageal reflux disease. World J. Gasteroenterol. 21, 982-7 (2015),
  2. Chandra M et al. A role for the Ca2+ channel TRPML1 in gastric acid secretion, based on analysis of knockout mice. Gasteroenterology 140, 857-67 (2011),
  3. Kawamura YI et al. Introduction of Sd(a) carbohydrate antigen in gastrointestinal cancer cells eliminates selectin ligands and inhibits metastasis. Cancer Res. 65, 6220-7 (2005),
  4. Mimata A et al. Loss of E-cadherin in mouse gastric epithelial cells induces signet ring-like cells, a possible precursor lesion of diffuse gastric cancer. Cancer Sci. 102, 942-50 (2011)
References
  1. Katakai, T., et al., J. Immunol. 177, 7858-7867 (2006)
  2. Kawamura, Y. I., et al., Cancer Res. 65, 6220-6227 (2005)
  3. Murphy, K., et al., Int. Immunol. 15, 269-277 (2003)
  4. Laurie, K. L., et al., J. Immunol. 169, 2361-2367 (2002)
  5. Kimura, T., et al., J. Biochem. 131, 923-932 (2002)
  6. Kimura, T., et al., J. Biol. Chem. 277, 20671-20677 (2002)
  7. Biondo, M., et al., J. Immunol. 166, 2090-2099 (2001)
  8. Alderuccio, F., et al., Int. Immunol. 12, 343-352 (2000)
  9. Jones, C. M., et al., Gastroenterology 101, 287-294 (1991)
  10. Toh, B., et al., PNAS 87, 6418-6422 (1990)
  11. Mori, Y., et al., Gastroenterology 97, 364-375 (1989)