Products Product Type Proteins and Peptides Recombinant Protein CircuLex PCSK9 D374Y in culture medium

CircuLex PCSK9 D374Y in culture medium

CircuLex PCSK9 D374Y in culture medium.

Specifications:

Description

PCSK9 binds to the LDL receptor in vitro and in vitro. Human PCSK9 D374Y containing a C-terminal His-tag, expressed in HEK 293 cells. Binding capability to EGF-AB domain of LDL receptor was confirmed by using CircuLex PCSK9-LDLR in vitro Binding Assay Kit (CY-8150). The PCSK9 D374Y in culture medium might be used for LDLR degradation assay on HepG2 cell. Unused PCSK9 D374Y in culture medium should be stored at -70°C.

Target:	PCSK9
Product Type:	Recombinant Protein
Size:	50 µg
Research Area / Disease:	Metabolism

References

Seidah NG, Benjannet S, Wickham L, Marcinkiewicz J, Jasmin SB, Stifani S, Basak A, Prat A, Chretien M (2003) Proc Natl Acad Sci USA 100:928–933.
Abifadel M, Varret M, Rabes JP, Allard D, Ouguerram K, Devillers M, Cruaud C, Benjannet S, Wickham L, Erlich D, et al. (2003) Nat Genet 34:154–156.
Leren TP (2004) Clin Genet 65:419–422.
Allard D, Amsellem S, Abifadel M, Trillard M, Devillers M, Luc G, Krempf M, Reznik Y, Girardet JP, Fredenrich A, et al. (2005) Hum Mutat 26:497.
Cohen JC, Boerwinkle E, Mosley TH, Jr, Hobbs HH (2006) N Engl J Med 354, 1264–1272.
Berge KE, Ose L, Leren TP (2006) Arterioscler Thromb Vasc Biol 26:1094–1100.
Maxwell KN, Breslow JL (2004) Proc Natl Acad Sci USA 101:7100–7105.
Rashid S, Curtis DE, Garuti R, Anderson NN, Bashmakov Y, Ho YK, Hammer RE, Moon YA, Horton JD (2005) Proc Natl Acad Sci USA 102:5374–5379.
Lagace, T. A., Curtis, D. E., Garuti, R., McNutt, M. C., Park, S. W., Prather, H. B., Anderson, N. N., Ho, Y. K., Hammer, R. E., and Horton, J. D. (2006) J. Clin. Investig. 116, 2995–3005 10. Cameron, J., Holla, O. L., Ranheim, T., Kulseth, M. A., Berge, K. E., and Leren, T. P. (2006) Hum. Mol. Genet. 15, 1551–1558
Zhang, D. W., Lagace, T. A., Garuti, R., Zhao, Z., McDonald, M., Horton, J. D., Cohen, J. C., and Hobbs, H. H. (2007) J. Biol. Chem. 282, 18602–18612
Hyock Joo Kwon*, Thomas A. Lagace†, Markey C. McNutt†, Jay D. Horton†‡, and Johann Deisenhofer (2008) ) Proc Natl Acad Sci USA 105: 1820–1825.