Anti-Phospho-MBS (MYPT1) (Thr853) pAb

  • Applications
    • ELISA
    • WB
  • Target MBS
  • Host Species Rabbit
  • Species Reactivities Chicken, Human, Mouse, Rat
  • Code # CY-P1025
  • Size 50 µg
  • Price
    $289.63
Specifications

Background

The expression of a constitutively active form of Rho-kinase can induce stress fiber and focal adhesion formation in fibroblasts and an increase in the level of myosin light chain (MLC20) phosphorylation in smooth muscle and in non-muscle cells (1). These results are attributed to an increase in MLC20 phosphorylation and this is thought to reflect the inhibition of myosin phosphatase (MP). MP is composed of three subunits: a catalytic subunit of type 1 phosphatase delta isoform, PP1c delta, and two non-catalytic subunits, 110 and 20 kDa. The 110 kDa subunit is a targeting molecule and has been termed myosin phosphatase target subunit 1 (MYPT1) or myosin-binding subunit (MBS). MBS/MYPT1 is the key molecule involved in regulation of MP activity (2). Phosphorylation by Rho-kinase inhibits MP activity and this reflects a decrease in Vmax. Activity of MP with different substrates also is inhibited by phosphorylation. Two major sites of phosphorylation on chicken MBS/MYPT1 are Thr696 and Thr853. Various point mutations were designed for these phosphorylation sites. Following thiophosphorylation by Rho-kinase and assays of phosphatase activity it was determined that Thr695 was responsible for inhibition. Rho-kinase, which is activated by GTP-RhoA, phosphorylates MBS/MYPT1 and consequently inactivates myosin phosphatase (MP). Over-expression of RhoA or activated RhoA in NIH 3T3 cells increases phosphorylation of MBS/MYPT1 and MLC20. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase.
  • Antibody Type:
    Polyclonal
  • Application:
    ELISA, WB
  • Concentration:
    1.0 mg/mL
  • Conjugate:
    Unlabeled
  • Description:

    Polyclonal Antibody of 50 µg targeting MBS for ELISA, WB.

  • Formulation:
    Supplied in 20mM phosphatase buffer (pH 7.5), 300mM NaCl, 50% glycerol.
  • Host Species:
    Rabbit
  • Immunogen:
    Thr853 phosphorylation of synthetic peptide near MBS/MYPT1 (binding KLH)
  • Isotype:
    IgG
  • Product Type:
    Antibody
  • Research Area:
    Cell Biology
  • Short Description:

    Anti-Phospho-MBS (MYPT1) (Thr853) Polyclonal Antibody.

  • Size:
    50 µg
  • Species Reactivity:
    Chicken, Human, Mouse, Rat
  • Storage Temperature:
    -20°C
  • Target:
    MBS
Citations
  1. Gabrielli L et al. Increased rho-kinase activity in hypertensive patients with left ventricular hypertrophy. Am J Hypertens. 27, 838-45 (2014),
  2. Isgro J et al. Enhanced rho-associated protein kinase activation in patients with systemic lupus erythematosus. Arthritis Rheum. 65, 1592-602 (2013)
References
  1. Kimura, K., et al. Regulation of myosin phosphatase by Rho and Rho-associated kinase (Rho-kinase). Science 273: 245-248, 1996.
  2. Feng, J., et al. Inhibitory Phosphorylation site for Rho-Kinase on Smooth Muscle Myosin Phosphatase J. Biol. Chem. 274: 37385-37390, 1999