Anti-RAGE (Human) mAb

  • Applications
    • ELISA
    • FCM
    • WB
  • Target RAGE
  • Host Species Mouse
  • Species Reactivities Human
  • Code # CY-M1038
  • Size 100 μg
  • Price
    $501.28
Specifications

Background

RAGE is a multi-ligand member of the immunoglobulin superfamily of cell surface molecules that is expressed in a variety of cell lines, including end othelial cells, smooth muscle cells, mononuclear phagocytes, pericytes, neurons, cardiac myocytes, mesangial cells and hepatocytes (1, 2). RAGE interacts with different structures to transmit a signal into the cell and recognizes three-dimensional structures rather than specific amino acid sequences. Therefore, RAGE seems to fulfill the requirements of a pattern-recognition receptor. As a member of the immunoglobulin superfamily, it interacts with a diverse class of ligands, including AGEs (3, 4), HMGB1 (also known as Amphoterin) (5), amyloid β-peptide (6), amyloid A (7), leukocyte adhesion receptors (8), prions (9), Escherichia coli curli operons (10), β-sheet fibrils (11) and several members of the S100 protein superfamily including S100/calgranulins (12). Thus RAGE may have potential involvement in several pathological processes including inflammation, diabetes, Alzheimer’s disease (AD), systemic amyloidosis, and tumor growth (13). It has been reported that soluble RAGE (sRAGE) isoforms that could act as decoy receptors may be cleaved proteolytically from the native RAGE expressed on the cell surface (14), as well as endogenous secretory RAGE (esRAGE) derived from a splice variant, carrying all of the extracellular domains. This proteolytic generation of sRAGE was initially descr ibed as occurring in mice (15). These diverse types of sRAGE in human plasma could exert significant protective effects against RAGE-mediated toxicity.
  • Antibody Type:
    Monoclonal
  • Application:
    ELISA, FCM, WB
  • Clone Number:
    YK-2B4
  • Concentration:
    1.0 μg/μL
  • Conjugate:
    Unlabeled
  • Description:

    Monoclonal Antibody of 100 μg targeting RAGE for ELISA, FCM, WB.

  • Formulation:
    20 mM phosphate buffer (pH 7.5), 300 mM NaCl, 50 % glycerol.
  • Host Species:
    Mouse
  • Immunogen:
    Extracellular domain of Recombinant human RAGE
  • Isotype:
    IgG1
  • Product Type:
    Antibody
  • Research Area:
    Metabolism
  • Short Description:

    Anti-RAGE (Human) Monoclonal Antibody.

  • Size:
    100 μg
  • Species Reactivity:
    Human
  • Storage Temperature:
    -20°C
  • Target:
    RAGE
References
  1. Neeper M, Schmidt AM, Brett J et al.. J Biol Chem 1992, 267: 14998-5004. 
  2. Brett J, Schmidt AM, Yan SD et al. Am J Pathol 1993, 143: 1699-712. 
  3. Neeper M, Schmidt AM, Brett J, et al. J Biol Chem 1992, 267: 14998-15004. 
  4. Schmidt AM, Vianna M, Gerlach M, et al. J Biol Chem 1992, 267: 14987-14997 
  5. Hori O, Brett J, Slattery T, Cao R et al. J Biol Chem 1995, 270: 25752-25761. 
  6. Yan SD, Zhu H, Fu J, Yan SF, Roher A et al. Proc Natl Acad Sci U S A 1997, 94: 5296-5301. 
  7. Yan SD, Zhu H, Zhu A, Golabek A, Du H, Roher A, 
  8. Yu J, Soto C, Schmidt AM, Stern D, et al. Nat  Med 2000, 6: 643-651. 
  9. Chavakis T, Bierhaus A, Al-Fakhri N et al. J Exp Med 2003, 198: 1507-1515. 
  10. Sasaki N, Takeuchi M, Chowei H, Kikuchi S et al Neurosci Lett 2002, 326: 117-120. 
  11. Chapman MR, Robinson LS, Pinkner JS et al. Science 2002, 295: 851-855 
  12. Bierhaus A, Humpert PM, Morcos M, et al. J Mol Med 2005, 83: 876- 886. 
  13. Hofmann MA, Drury S, Fu C, Qu W et al. Cell 199 9, 97: 889-901. 
  14. Stern DM, Yan SD, Yan SF, and Schmidt AM. Ageing Res Rev. 2002, 1: 1-15. 
  15. 1Hudson, B.I., Harja, E., Moser, B. and Schmidt, A.M. Arterioscler Thromb Vasc. Biol. 2005, 25: 879–82. 
  16. Hanford, L.E., Enghild, J.J. et al. J. Biol. Chem. 2004, 279: 50019–24