Anti-GSK-3α mAb

  • Applications
    • WB
  • Target GSK-3α
  • Host Species Mouse
  • Species Reactivities Human, Mouse, Rat
  • Code # CY-M1031
  • Size 100 μg
  • Price
    $401.02
Specifications

Alternative Names

glycogen synthase kinase-3 alpha

Background

The serine/threonine proline-directed kinase, glycogen synthase kinase-3 (GSK-3) plays a central role in a wide variety of normal and pathological cellular processes. First described as the kinase capable of phosphorylating and inactivating glycogen synthase, GSK-3 is now recognized as a key regulator of many normal processes such as cellular development, proliferation, and survival. Dysregulation of GSK-3 activity has been linked to various human diseases including Alzheimer's disease, diabetes, cancer, heart disease, schizophrenia, and mood disorders. It is generally considered constitutively active and is found as two isoforms, α and β (51 and 47 kDa, respectively), that have 97% sequence homology in their catalytic domains, and generally (but not always) have similar biological effects. GSK-3 can be regulated either by Wnt signaling or by the phosphatidylinositol (PI) 3-kinase/Akt pathways. Wnt signaling leads to an inhibition of the phosphorylation of β-catenin by GSK-3 and a corresponding increase in transcriptional activation of beta-catenin/Tcf target genes (1). Activation of PI3-kinase in response to stimulation of cells by insulin or growth factor causes a decrease in GSK-3 activity because of an inhibitory phosphorylation by Akt of GSK-3β on serine 9 or GSK-3αon serine 21 (2). The resulting inhibition of GSK-3 plays a critical role in signaling cell growth and survival downstream of PI3-kinase. In the absence of growth factor stimulation, GSK-3 is active and can inhibit cell proliferation as well as induce apoptosis (3–6). The substrates of GSK-3 that have been implicated in the regulation of cell proliferation and survival include the translation initiation factor eIF2B (7, 8), cyclin D1 (3), the Bcl-2 family member Mcl-1 (9), and a variety of transcription factors that are phosphorylated by GSK-3 either in vitro or in vivo (5, 10).
  • Antibody Type:
    Monoclonal
  • Application:
    WB
  • Clone Number:
    MS-1G7
  • Concentration:
    1.0 mg/mL
  • Conjugate:
    Unlabeled
  • Description:

    Monoclonal Antibody of 100 μg targeting GSK-3alpha for WB.

  • Formulation:
    Supplied in 20mM phosphatase buffer (pH 7.5), 300mM NaCl, 50% glycerol.
  • Host Species:
    Mouse
  • Immunogen:
    Recombinant human full-length GSK-3alpha
  • Isotype:
    IgG1
  • Product Type:
    Antibody
  • Research Area:
    Cell Biology
  • Short Description:

    Anti-GSK-3 alpha Monoclonal Antibody.

  • Size:
    100 μg
  • Species Reactivity:
    Human, Mouse, Rat
  • Storage Temperature:
    -20°C
  • Target:
    GSK-3α
References
  1. Logan, C. Y., and Nusse, R. (2004) Annu. Rev. Cell Dev. Biol. 20, 781–810
  2. Cross, D. A., Alessi, D. R., Cohen, P., Andjelkovich, M., and Hemmings, B. A. (1995) Nature 378, 785–789
  3. Diehl, J. A., Cheng, M., Roussel, M. F., and Sherr, C. J. (1998) Genes Dev. 12, 3499–3511
  4. Frame, S., and Cohen, P. (2001) Biochem. J. 359, 1–16
  5. Jope, R. S., and Johnson, G. V. (2004) Trends Biochem. Sci. 29, 95–102
  6. Pap, M., and Cooper, G. M. (1998) J. Biol. Chem. 273, 19929–19932
  7. Hardt, S. E., Tomita, H., Katus, H. A., and Sadoshima, J. (2004) Circ. Res. 94, 926–935
  8. Pap, M., and Cooper, G. M. (2002) Mol. Cell. Biol. 22, 578–586
  9. Maurer, U., Charvet, C., Wagman, A. S., Dejardin, E., and Green, D. R. (2006) Mol. Cell 21, 749–760
  10. Doble, B. W., and Woodgett, J. R. (2003) J. Cell Sci. 116, 1175–1186