Anti-Adenovirus Hexon mAb

  • Applications
    • ELISA
    • FCM
    • ICC
    • IF
  • Target Adenovirus Hexon
  • Host Species Mouse
  • Code # CY-M1027
  • Size 50 µg
  • Price
    $284.06
Specifications

Background

Recombinant baculoviruses derived from the Autographa californica nuclear polyhedrosis virus (AcNPV) are widely used to express heterologous genes in insect cells, but the use of the baculovirus expression vector system is hampered by slow and tedious procedures for the selection and propagation of baculovirus and for titer determination. Titration of baculoviral stocks is important because it is critical to have consistency between samples, and to achieve the right level of transient expression. Moreover, it is important to know the titer of infectious particles when producing viral stocks for successful virus production. This antibody can be used for determining titers of baculovirus stocks by immunocytochemical or immunofluorescence methods.
  • Antibody Type:
    Monoclonal
  • Application:
    ELISA, FCM, ICC, IF
  • Clone Number:
    AF-3H12
  • Concentration:
    1.0 mg/mL
  • Conjugate:
    Unlabeled
  • Description:

    Monoclonal Antibody of 50 µg targeting Adenovirus Hexon for ELISA, FCM, ICC, IF.

  • Formulation:
    Supplied in 20mM phosphatase buffer (pH 7.5), 300mM NaCl, 50% glycerol.
  • Host Species:
    Mouse
  • Immunogen:
    Wild-type adenovirus type 5 particles
  • Isotype:
    IgG2a
  • Product Type:
    Antibody
  • Reactivity:
    Adenovirus Hexon antibody detects the Hexon protein of adenovirus (Type5) .
  • Research Area:
    Cell Biology
  • Short Description:

    Anti-Adenovirus Hexon Monoclonal Antibody.

  • Size:
    50 µg
  • Storage Temperature:
    -20°C
  • Target:
    Adenovirus Hexon
References
  1. Aiello, L., Guilfoyle, R., Huebner, K. & Weinmann, R. (1979) Adenovirus 5 DNA sequences transcribed in transformed human embryo kidney cells (HEK-Ad5 or 293). Virology 94: 460–469
  2. Eykholt, R. L., Mitchell, M. D. & Marvin K. W. (2000) Accelerated Titering of Adenoviruses. BioTechniques 28: 871–873
  3. Graham, F. L., Smiley, J., Russel, W. C. & Nairn, R. (1977) Characterization of a human cell line transformed by DNA from human adenovirus type 5. J. Gen. Virol. 36: 59–72
  4. Price, J., Turner, D., Cepko C. (1987) Lineage analysis in the vertebrate nervous system by retrovirus-mediated gene transfer. Proc. Natl. Acad. Sci. USA 84(1): 156–160