Anti-Baculovirus Envelope gp64 mAb

  • Applications
    • ELISA
    • FCM
    • ICC
    • IF
  • Target Baculovirus Envelope gp64
  • Host Species Mouse
  • Code # CY-M1026
  • Size 50 µg
  • Price
    $284.06
Specifications

Background

Recombinant baculoviruses derived from the Autographa californica nuclear polyhedrosis virus (AcNPV) are widely used to express heterologous genes in insect cells, but the use of the baculovirus expression vector system is hampered by slow and tedious procedures for the selection and propagation of baculovirus and for titer determination. Titration of baculoviral stocks is important because it is critical to have consistency between samples, and to achieve the right level of transient expression. Moreover, it is important to know the titer of infectious particles when producing viral stocks for successful virus production. This antibody can be used for determining titers of baculovirus stocks by immunocytochemical or immunofluorescence methods.
  • Antibody Type:
    Monoclonal
  • Application:
    ELISA, FCM, ICC, IF
  • Clone Number:
    AF-2C8
  • Concentration:
    1.0 mg/mL
  • Conjugate:
    Unlabeled
  • Description:

    Monoclonal Antibody of 50 µg targeting Baculovirus Envelope gp64 for ELISA, FCM, ICC, IF.

  • Formulation:
    Supplied in 20mM phosphatase buffer (pH 7.5), 300mM NaCl, 50% glycerol.
  • Host Species:
    Mouse
  • Immunogen:
    Wild-type AcNPV particles
  • Isotype:
    IgG2a
  • Product Type:
    Antibody
  • Reactivity:
    Baculovirus envelope gp64 antibody detects the gp64 envelope protein ofbaculovirus Autographa californica nuclear polyhedrosis virus (AcNPV) .
  • Research Area:
    Cell Biology
  • Short Description:

    Anti-Baculovirus Envelope gp64 Monoclonal Antibody.

  • Size:
    50 µg
  • Storage Temperature:
    -20°C
  • Target:
    Baculovirus Envelope gp64
References
  1. Hohmann, A. W. and P. Faulkner. 1983. Monoclonal antibodies to baculovirus structural proteins: determination of specificities by Western blot analysis. Virology 125(2): 432-44
  2. Volkman, L. E. and P. A. Goldsmith. 1988. Resistance of the 64K protein of budded Autographa californica nuclear polyhedrosis virus to functional inactivation by proteolysis. Virology 166(1): 285-9
  3. Blissard, G. W. and G. F. Rohrmann 1989. Location, sequence, transcriptional mapping, and temporal expression of the gp64 envelope glycoprotein gene of the Orgyia pseudotsugata multicapsid nuclear polyhedrosis virus. Virology 170(2): 537-55
  4. (Plonsky, I., M. S. Cho, et al. (1999). An analysis of the role of the target membrane on the Gp64-induced fusion pore. Virology 253(1): 65-76