Anti-Phospho-CPI-17 (Thr38) mAb (Monoclonal Antibody)

Anti-Phospho-CPI-17 (Thr38) Monoclonal Antibody.

Specifications:

Description

Monoclonal Antibody of 100 μg targeting CPI-17 for ELISA, WB.

Target: CPI-17
Product Type: Antibody
Size: 100 μg
Application: ELISA, WB
Research Area / Disease: Cell Biology
Conjugate: Unlabeled
Antibody Type: Monoclonal
Clone Number: AK-1F11
Concentration: 1.0 mg/mL
Formulation: Supplied in 20mM phosphatase buffer (pH 7.5), 300mM NaCl, 50% glycerol.
Isotype: IgG1
Immunogen: Near the Thr38 phosphorylated peptide synthesis of human CPI-17 (binding KLH)
Host Species: Mouse
Species Reactivity: Human, Mouse, Rat
Source: This monoclonal antibody (clone AK-1F11) was produced by immunizing mice with a synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Thr38 of human CPI-17. The IgG fraction was purified by protein A-Sepharose chromatography.
Reactivity: Phospho-CPI-17 Thr38 Antibody detects phosphorylated recombinant CPI-17 whenphosphorylated at threonine38 in vitro. The antibody doesnot recognize the unphosphorylated CPI-17 protein. Theantibody detects the 17 kDa phospho-CPI-17 by Westernblotting a
Storage Temperature: '-20°C
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.

References

  1. Hirano K, et al. Protein kinase network in the regulation of phosphorylation and dephosphorylation of smooth muscle myosin light chain. Mol. Cell Biochem. 248:105-14 (2003).
  2. Somlyo AP, Somlyo AV. Signal transduction by G-proteins, rho-kinase and protein phosphatase to smooth muscle and non-muscle myosin II. J Physiol. 522:177-85 (2000).
  3. Niiro N, et al. Agonist-induced changes in the phosphorylation of the myosin-binding subunit of myosin light chain phosphatase and CPI17, two regulatory factors of myosin light chain phosphatase, in smooth muscle. Biochem J. 369:117-28 (2003).
  4. Kitazawa T, et al. Phosphorylation of the myosin phosphatase targeting subunit and CPI-17 during Ca2+ sensitization in rabbit smooth muscle. J. Physiol. 546:879-89 (2003).
  5. Kitazawa T, et al. Agonists trigger G protein-mediated activation of the CPI-17 inhibitor phosphoprotein of myosin light chain phosphatase to enhance vascular smooth muscle contractility. J. Biol. Chem. 275:9897-900 (2000)
  6. Woodsome TP, et al. Expression of CPI-17 and myosin phosphatase correlates with Ca(2+) sensitivity of protein kinase C-induced contraction in rabbit smooth muscle. J. Physiol. 535:553-64 (2001).
  7. Deng JT, et al. Phosphorylation of the myosin phosphatase inhibitors, CPI-17 and PHI-1, by integrin-linked kinase. Biochem J. 367:517-24 (2002)
  8. Li L, et al. Possible involvement of the novel CPI-17 protein in protein kinase C signal transduction of rabbit arterial smooth muscle. J. Physiol. 508:871-81 (1998).
  9. Kitazawa T, et al. Agonists trigger G protein-mediated activation of the CPI-17 inhibitor phosphoprotein of myosin light chain phosphatase to enhance vascular smooth muscle contractility. J. Biol. Chem. 275: 9897-900 (2000)
Code CY-M1024
Product Type Antibody
Size 100 μg
Application ELISA, WB
Price
$401.02
Qty: