Anti-Phospho-Lats2 (Ser83) mAb

  • Applications
    • ELISA
    • WB
  • Target Lats2
  • Host Species Mouse
  • Species Reactivities Human
  • Code # CY-M1020
  • Size 100 μg
  • Price
    $401.02
Specifications

Background

Lats2 is a new member of the Lats tumor suppressor family. The human LATS2 gene is located at chromosome 13q11-12, which has been shown to be a hot spot (67%) for LOH in nonsmall cell lung cancer. The Drosophila “large tumor suppressor” (lats) gene encodes a putative serine/threonine kinase. By screening a human testis cDNA library with a mouse LATS2 cDNA, Yabuta et al. isolated a partial human LATS2 cDNA encoding a deduced 1,046-amino acid C-terminal partial protein. LATS2 contains a PAPA repeat consisting of 7 copies of the dipeptide proline-alanine, which may be involved in protein-protein interactions, and a C-terminal Ser/Thr kinase domain. Analysis of the amino acid sequences of the human and mouse LATS2 kinase domains indicated that the LATS2 proteins are most closely related to the LATS1 serine/threonine kinases, followed by Drosophila Lats. Immunoblot analysis of human cell lines showed that endogenous LATS2 is a nuclear protein of approximately 125 kDa. Northern blot analysis detected a 5.8-kb LATS2 transcript in several human tissues, with highest expression in heart and skeletal muscle.
  • Antibody Type:
    Monoclonal
  • Application:
    ELISA, WB
  • Clone Number:
    ST-3B11
  • Concentration:
    1.0 mg/mL
  • Conjugate:
    Unlabeled
  • Description:

    Monoclonal Antibody of 100 μg targeting Lats2 for ELISA, WB.

  • Formulation:
    Supplied in 20mM phosphatase buffer (pH 7.5), 300mM NaCl, 50% glycerol.
  • Host Species:
    Mouse
  • Immunogen:
    Near the Ser83 phosphorylated peptide synthesis of human Lats2 (binding KLH)
  • Isotype:
    IgG1
  • Product Type:
    Antibody
  • Reactivity:
    Recognizes phosphorylated Lats2 in human cell lysates. Not tested in other species.
  • Research Area:
    Cell Biology
  • Short Description:

    Anti-Phospho-Lats2 (Ser83) Monoclonal Antibody.

  • Size:
    100 μg
  • Species Reactivity:
    Human
  • Storage Temperature:
    -20°C
  • Target:
    Lats2
Citations
  1. Emery A, Sorrell DA, Lawrence S, Easthope E, Stockdale M, Jones DO, Zheleva D, Scaerou F, Glover DM.; A Novel Cell-Based, High-Content Assay for Phosphorylation of Lats2 by Aurora A. J Biomol Screen. 16: 925-31, 2011
References
  1. Tao, W.; et al. Human homologue of the Drosophila melanogaster lats tumor suppressor modulates CDC2 activity. Nature Genet. 21, 177-181, 1999.
  2. Li Y, et al. Lats2, a putative tumor suppressor, inhibits G1/S transition. Oncogene. 22(28):4398-405, 2003
  3. Ishizaki K, et al. Frequent polymorphic changes but rare tumor specific mutations of the LATS2 gene on 13q11-12 in esophageal squamous cell carcinoma. Int. J. Oncol. 21(5):1053-7, 2002
  4. Yabuta N, et al. Structure, expression, and chromosome mapping of LATS2, a mammalian homologue of the Drosophila tumor suppressor gene lats/warts. Genomics 63(2):263-70, 2000