Anti-Phospho-LSP1 (Ser204) mAb

  • Applications
    • ELISA
    • WB
  • Target LSP1
  • Host Species Mouse
  • Species Reactivities Human, Mouse, Rat
  • Code # CY-M1019
  • Size 100 μg
  • Price
    $389.88
Specifications

Alternative Names

leukocyte specific protein 1

Background

Signal transduction in B lymphocytes following antigen binding to a membrane-associated immunoglobulin complex initiates a diverse array of intracellular events. To define genes participating in these events, May et al. (1993) and others used various cDNA cloning strategies to identify genes preferentially expressed in B lymphocytes. One gene, variously named LSP1 and pp52, has a number of properties supporting a possible role in B-cell signaling pathways. LSP1 (The leukocyte specific protein 1) is a multi functional protein involved in such divers biological processes as the regulation of neutrophil motility, chemotaxis, adhesion and membrane immunoglobulin M (mIgM) mediated apoptosis of B-lymphocytes. The 330-amino-acid mouse LSP1 protein contains a high-affinity F-actin binding site and in intact cells localizes to the F-actin filament containing cytoskeleton.
  • Antibody Type:
    Monoclonal
  • Application:
    ELISA, WB
  • Clone Number:
    AT-1E6
  • Concentration:
    1.0 mg/mL
  • Conjugate:
    Unlabeled
  • Description:

    Monoclonal Antibody of 100 μg targeting LSP1 for ELISA, WB.

  • Formulation:
    Supplied in 20mM phosphatase buffer (pH 7.5), 300mM NaCl, 50% glycerol.
  • Host Species:
    Mouse
  • Immunogen:
    Near the Ser204 phosphorylated peptide synthesis of human LSP1 (binding KLH)
  • Isotype:
    IgG1
  • Product Type:
    Antibody
  • Reactivity:
    Phospho-LSP1 Ser204 Antibody detects phosphorylated recombinant LSP1 by Westernblotting only when phosphorylated at serine204. Theantibody detects the 52 kDa Phospho-LSP1 by Westernblotting analysis.
  • Research Area:
    Cell Biology
  • Short Description:

    Anti-Phospho-LSP1 (Ser204) Monoclonal Antibody.

  • Size:
    100 μg
  • Species Reactivity:
    Human, Mouse, Rat
  • Storage Temperature:
    -20°C
  • Target:
    LSP1
Citations
  1. Hossain M et al. ICAM-1-mediated leukocyte adhesion is critical for the activation of endothelial LSP1. Am J Physiol Cell Physiol. 304, C895-904 (2013)
References
  1. May, W., et al. Human lymphocyte-specific pp52 gene is a member of a highly conserved dispersed family. Genomics 15: 515-520, 1993.
  2. Huang CK, et al. LSP1 is the major substrate for mitogen-activated protein kinase-activated protein kinase 2 in human neutrophils. J Biol. Chem. 272(1): 17-9, 1997
  3. Jongstra-Bilen J, et al. The lymphocyte-specific protein LSP1 binds to F-actin and to the cytoskeleton through its COOH-terminal basic domain. J Cell Biol. 118(6): 1443-53, 1992
  4. Jongstra-Bilen J, et al. Human and mouse LSP1 genes code for highly conserved phosphoproteins. J Immunol. 144(3): 1104-10, 1990