Anti-Phospho-Rb (Ser807) mAb

  • Applications
    • ELISA
    • IP
    • WB
  • Target pRB
  • Host Species Mouse
  • Species Reactivities Human
  • Code # CY-M1015
  • Size 100 μg
  • Price
    $345.32
Specifications

Alternative Names

Retinoblastoma protein

Background

The retinoblastoma protein (Rb) is a nuclear phosphoprotein that regulates growth in the G1 phase of the cell cycle. Rb exerts its growth-inhibitory effects in part by binding to and inhibiting critical regulatory proteins, including members of the E2F family of transcription factors; E2F activation is necessary for the G1-S transition. E2F selectively associates with hypophosphorylated Rb, and phosphorylation of Rb appears to release E2F from an inhibitory complex, enabling it to promote the transcription necessary for progression into late G1 and S. Rb is phosphorylated on a still imprecisely defined number of threonine and serine residues during G1 (1, 2). Both Ser608) and Ser780 have been identified as among the sites that are initially phosphorylated. These phosphorylations have distinct effects on the ability of Rb to interact with its various partner proteins. Thus, Rb phosphorylated on Ser780 appears to lose its ability to bind to E2F. Phosphorylation of Ser807 and/or Ser811 is required to abolish Rb binding to c-Abl, while modification of Thr821) and/or Thr826 is required to abolish Rb binding to LXCXE-containing proteins such as simian virus 40 large T antigen. However, these four sites do not appear to be involved in regulating Rb binding to the E2F transcription factors. Phosphorylation of Rb also has effects on cell physiology, ostensibly by changing its association with these and other interacting partner proteins. For example, phosphorylation of Ser795 is required to inactivate Rb-imposed growth suppression in a microinjection assay. However, the relationship between growth inhibition and E2F binding is complex: phosphorylation of Rb in vitro by cyclin D-, cyclin E-, or cyclin A-associated kinase has been reported to release E2F, yet only action by cyclin D1–cyclin-dependent kinase 4 (cdk4) complexes, but not by cyclin E-cdk2 complexes, abrogates the growth-inhibitory property of Rb when microinjected into SaOS-2 cells.
  • Antibody Type:
    Monoclonal
  • Application:
    ELISA, IP, WB
  • Clone Number:
    5H12
  • Concentration:
    1.0 mg/mL
  • Conjugate:
    Unlabeled
  • Description:

    Monoclonal Antibody of 100 μg targeting pRB for ELISA, IP, WB.

  • Formulation:
    Supplied in 20mM phosphatase buffer (pH 7.5), 300mM NaCl, 50% glycerol.
  • Host Species:
    Mouse
  • Immunogen:
    Near the Ser807 phosphorylated peptide synthesis of human Rb (binding KLH)
  • Isotype:
    IgG1
  • Product Type:
    Antibody
  • Reactivity:
    The Phospho-Rb Ser612 Antibodydetects endogenous Rb protein only when phosphorylatedat Ser807. Recognizes human phospho-Ser807 Rb protein(~115 kDa) ; not tested in other species.
  • Research Area:
    Cancer
  • Short Description:

    Anti-Phospho-Rb (Ser807) Monoclonal Antibody.

  • Size:
    100 μg
  • Species Reactivity:
    Human
  • Storage Temperature:
    -20°C
  • Target:
    pRB
References
  1. Kitagawa, M et al. The consensus motif for phosphorylation by cyclin D1-Cdk4 is different from that for phosphorylation by cyclin A/E-Cdk2 EMBO J. 15: 7060-7069, 1996
  2. Suzuki, S et al. Enzyme-Linked Immunosorbent Assay for Distinct Cyclin-Dependent Kinase Activities Using Phosphorylation-Site-Specific Anti-Rb Monoclonal Antibodies. Anal. Biochem. 301(1):65-74, 2002
  3. Taya, Y et al. Generation and Application of Phospho-specific Antibodies for p53 and Rb Methods in Molecular Biology Vol. 223, 17-26: Tumor Suppressor Genes: Regulation and Function, and Medical Applications