Anti-Phospho-MBS (MYPT1) (Thr696) mAb

  • Applications
    • ELISA
    • WB
  • Target MBS/MYPT1
  • Host Species Mouse
  • Species Reactivities Chicken, Human, Mouse, Rat
  • Code # CY-M1011
  • Size 100 μg
  • Price
    $401.02
Specifications

Background

The expression of a constitutively active form of Rho-kinase induced stress fiber and focal adhesion formation in fibroblasts and an increase in the level of myosin light chain (MLC20) phosphorylation (1). These results in smooth muscle and in non-muscle cells are attributed to an increase in MLC20 phosphorylation and this is thought to reflect the inhibition of myosin phosphatase (MP). MP is composed of three subunits: a catalytic subunit of type 1 phosphatase delta isoform, PP1c delta, and two non-catalytic subunits, 110 and 20 kDa. The 110-kDa subunit is a targeting molecule and thus has been termed myosin phosphatase target subunit 1 (MYPT1) or myosin-binding subunit (MBS). MYPT1/ MBS is the key molecule involved in regulation of MP activity (2). Phosphorylation by Rho-kinase inhibited MP activity and this reflected a decrease in Vmax. Activity of MP with different substrates also was inhibited by phosphorylation. Two major sites of phosphorylation on chicken MYPT1/ MBS were Thr695 and Thr850. Various point mutations were designed for these phosphorylation sites. Following this phosphorylation by Rho-kinase and assays of phosphatase activity it was determined that Thr695 was responsible for inhibition. Rho-kinase, which is activated by GTP-RhoA, phosphorylated MYPT1/ MBS and consequently inactivated myosin phosphatase (MP). Over-expression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MYPT1/ MBS and MLC20. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase.
  • Antibody Type:
    Monoclonal
  • Application:
    ELISA, WB
  • Clone Number:
    AF20
  • Concentration:
    1.0 mg/mL
  • Conjugate:
    Unlabeled
  • Description:

    Monoclonal Antibody of 100 μg targeting MBS/MYPT1 for ELISA, WB.

  • Formulation:
    Supplied in 20mM phosphatase buffer (pH 7.5), 300mM NaCl, 50% glycerol.
  • Host Species:
    Mouse
  • Immunogen:
    Synthetic peptide-KLH phosphorylation of Thr696 of human near MBS/MYPT1
  • Isotype:
    IgG1
  • Product Type:
    Antibody
  • Reactivity:
    Phospho-MBS/MYPT1 Thr696Antibody detects endogenous MBS/ MYPT1 only whenphosphorylated at threonine696. The antibody does notrecognize other myosin phosphatase regulatory subunits.The antibody detects the ~115 kDa MBS protein byWestern blotting analy
  • Research Area:
    Cell Biology
  • Short Description:

    Anti-Phospho-MBS (MYPT1) (Thr696) mAb.

  • Size:
    100 μg
  • Species Reactivity:
    Chicken, Human, Mouse, Rat
  • Storage Temperature:
    -20°C
  • Target:
    MBS/MYPT1
Citations
  1. Ocaranza MP, Gabrielli L, Mora I, Garcia L, McNab P, Godoy I, Braun S, C_rdova S, Castro P, Novoa U, Chiong M, Lavandero S, Jalil JE.; Markedly increased Rho-kinase activity in circulating leukocytes in patients with chronic heart failure. Am Heart J. 161: 931-7, 2011
References
  1. Kimura, K et al. Regulation of myosin phosphatase by Rho and Rho-associated kinase (Rho-kinase). Science 273: 245-248, 1996
  2. Feng J et al. Inhibitory Phosphorylation site for Rho-Kinase on Smooth Muscle Myosin Phosphatase J.B.C. 274, 37385-37390, 1999