Aurora C Positive Control

  • Code # CY-E1174-2
  • Size 200 Assays
  • Price Call for Price
Specifications

Background

Human full length Aurora C, containing an N-terminal GST-tag, expressed in E. coli. Purified by GSH-agarose chromatography. The Aurora C Positive control is designed to use for Aurora Family Assay /Inhibitor Screening Kit [Cat# CY-1174]. The Aurora C Positive Control should be added to the well at 40 milliunits/well. For instance, dilute positive control 1:5, use 10 μL for 1 assay. Unused Aurora B Positive control should be stored in aliquots at -70°C.
  • Description:

    Aurora C Positive Control of 200 assays and Aurora C demonstrates a single 62 kDa band by SDSPAGE analysis.

  • Formulation:
    The Aurora C Positive Control is supplied frozen in abuffer containing 20mM HEPES-KOH (pH 7.5), 1% BSA,1 mM DTT, 50mM NaCl, 0.03% Brij35 and 50% glycerol.
  • Product Type:
    Recombinant Protein
  • Research Area:
    Cell Biology
  • Short Description:

    Aurora C Positive Control.

  • Size:
    200 Assays
  • Storage Temperature:
    -70°C
References
  1.  Francisco, L., Wang, W., and Chan, C.S. (1994). Type 1 protein phosphatase acts in opposition to IpL1 protein kinase in regulating yeast chromosome segregation. Mol. Cell Biol. 14, 4731–4740
  2. Leverson, J.D., Huang, H.K., Forsburg, S.L., and Hunter, T. (2002). The Schizosaccharomyces pombe aurora-related kinase Ark1 interacts with the inner centromere protein Pic1 and mediates chromosome segregation and cytokinesis. Mol. Biol. Cell 13, 1132–1143.
  3. Adams, R.R., Carmena, M., and Earnshaw, W.C. (2001a). Chromosomal passengers and the (aurora) ABCs of mitosis. Trends Cell Biol. 11, 49–54
  4. Nigg, E.A. (2001). Mitotic kinases as regulators of cell division and its checkpoints. Nat. Rev. Mol. Cell Biol. 2, 21–32
  5.  Kimura, M., Kotani, S., Hattori, T., Sumi, N., Yoshioka, T., Todokoro, K., and Okano, Y. (1997). Cell cycle-dependent expression and spindle pole localization of a novel human protein kinase, Aik, related to Aurora of Drosophila and yeast Ipl1. J. Biol. Chem. 272, 13766–13771
  6. Tsai, M.Y., Wiese, C., Cao, K., Martin, O., Donovan, P., Ruderman, J., Prigent, C., and Zheng, Y. (2003). A Ran signaling pathway mediated by the mitotic kinase Aurora A in spindle assembly. Nat. Cell Biol. 5, 242–248. 7. Terada, Y., Tatsuka, M., Suzuki, F., Yasuda, Y., Fujita, S., and Otsu, M. (1998). AIM-1: a mammalian midbody-associated protein required for cytokinesis. EMBO J. 17, 667–676
  7. Kimura, M., Matsuda, Y., Yoshioka, T., and Okano, Y. (1999). Cell cycle-dependent expression and centrosome localization of a third human aurora/Ipl1-related protein kinase, AIK3. J. Biol. Chem. 274, 7334–7340
  8. Bischoff, J.R., et al. (1998). A homologue of Drosophila aurora kinase is oncogenic and amplified in human colorectal cancers. EMBO J. 17, 3052–3065.
  9. Claire Ditchfield, Victoria L. Johnson, Anthony Tighe, Rebecca Ellston, Carolyn Haworth, Trevor Johnson, Andrew Mortlock, Nicholas Keen, and Stephen S. Taylor Aurora B couples chromosome alignment with anaphase by targeting BubR1, Mad2, and CENP-E to kinetochores. (2003) J. Cell Biol. 161: 267 - 280