CircuLex Human DJ-1/PARK7 ELISA Kit Ver.2

  • Applications
    • ELISA
  • Code # CY-9050V2
  • Size 96 Assays
  • Price
    $593.74
Specifications

Background

DJ-1 (PARK7/CAP1/RS) was originally cloned as a putative oncogene capable of transforming NIH-3T3 cells in cooperation with H-ras (1), a protein expressed in sperm (2), and a regulator of RNA-protein interactions (3). DJ-1 has also been isolated as a gene associated with autosomal early-onset Parkinson's disease (PD) (4). Taken together, DJ-1 appears to be involved in diverse biological processes (5). First, several lines of evidence suggest that DJ-1 plays a role in the oxidative stress response (6,7). In cu1tured mammalian ce11s, DJ-1 quenches reactive oxygen species and is converted into a variant with a more acidic isoelectric point (6,8). Therefore, DJ-1 protects against reactive oxygen species-induced cell death, and its suppression with small interfering RNA (siRNA) sensitizes cells to such insults (7-10). Second, DJ-1 modulates transcription through interaction with DJ-1-binding protein (11) as well as with protein inhibitor of activated STAT (PIAS) (12). The latter modulates the activity of various transcription factors, Third, DJ-1 has been recognized as a regulatory subunit of an RNA-binding protein (13), Fourth, DJ-1, which is structurally related to the molecular chaperone Hsp31, may have chaperone activity itself, preventing heat-induced aggregation of substrate proteins, including alpha-synuclein (14). In addition, severa1 1ines of evidence suggest that DJ-1 plays a role in human tumorigenesis. First, breast cancer patients have elevated levels of circulating DJ-1 and anti-DJ-1 autoantibodies compared to healthy and non-breast cancer patients (15). Secondly, DJ-1 protein is increased in primary non-small cell lung carcinoma samples (16). Thirdly, treatment of cells from the human lung cancer cell line NCI-H157 with paclitaxel and MEK inhibitor U0126 leads to a decrease in DJ-1 protein expression (16).
  • Application:
    ELISA
  • Components:
    • Microplate
    • 10X Wash Buffer
    • Dilution Buffer
    • Human DJ-1/PARK7 Standard
    • 20X HRP conjugated Detection Antibody
    • Conjugate Dilution Buffer
    • Substrate Reagent
    • Stop Solution
  • Description:

    The CycLex Research Product CircuLex Human DJ-1/PARK7 ELISA Kit Ver.2 is used for the quantitative and highly sensitive measurement of human DJ-1/PARK7 in serum, plasma, cell lysate and other biological samples.

  • Product Type:
    ELISA Kit
  • Research Area:
    Metabolism
  • Short Description:

    CircuLex Human DJ-1/PARK7 ELISA Kit.

  • Size:
    96 Assays
Citations
  1. An CN, Jiang H, Wang Q, Yuan RP, Liu JM, Shi WL, Zhang ZY, Pu XP.; Down-regulation of DJ-1 protein in the ejaculated spermatozoa from Chinese asthenozoospermia patients. Fertil Steril. 96: 19-23, 2011,
  2. Bande MF, Santiago M, Blanco MJ, Mera P, Capeans C, Rodr_guez-Alvarez MX, Pardo M, Pi_eiro A.; Serum DJ-1/PARK 7 Is a Potential Biomarker of Choroidal Nevi Transformation. Invest Ophthalmol Vis Sci. 53: 62-7. 2012,
  3. Satoshi Yamashita, Akira Mori, En Kimura, Shuji Mita, Yasushi Maeda, Teruyuki Hirano, Makoto Uchino; DJ-1 forms complexes with mutant SOD1 and ameliorates its toxicity. Journal of Neurochemistry 113: 860-870, 2010
References
  1. D. Nagakubo, T. Taira, H. Kitaura, M. lkeda, K. Tamai, S.M. Iguchi-Ariga and H. Ariga: Biochem. Biophys.Res.Commun.231, 509-513, 1997
  2. A. Wagenfeld, J. Gromoll and T.G, Cooper Biochem. Biophys. Res. Commun. 251, 545-549, 1998
  3. Y. Hod, S.N. Pentyala, T.C. Whyard and M.R. El-Maghrabi: J. Cell. Biochem. 72, 435-444, 1999
  4. Bonifati, V., Rizzu, P., van Baren, M. J., Schaap, O., Breedveld, G. J., Krieger, E., Dekker, M. C. J, Squitieri, F., Ibanez, P., Joosse, M., van Dongen, J.W., Vanacore, N., van Swieten, J.C, Brice, A., Meco, G., van Duijng, C. M., Oostra, B. A., Heutink, P.: Science 299, 256-259, 2003.
  5. Cookson, M. R,: Neuron 37, 7-10, 2003
  6. Mitsumoto, A.& Nakagawa, Y.: Free Radica1 Res.35, 885-893, 2001
  7. Canet-Aviles, R. M., Wilson, M. A., Mil1er, D. W., Ahmad, R., McLendon, C., Bandyopadhyay, S., Baptista, M. J., Ringe, D., Petsko, G. A. & Cookson, M. R.: Proc. Natl. Acad. Sci. USA 101, 9103-9108, 2004
  8. Taha, T., Saito,Y., Niki,T., Iguchi-Ariga,S.M., Takahashi, K. & Ariga, H.:,EMBO Rep.5, 213-218, 2004
  9. Martinat, C., Shendelrnan, S., Jonason, A., Leete, T., Bea1, M,F., Yang, L., Floss, T. & Abeliovich, A.: PLoS Biol, 2, e327, 2004
  10. Yokota, T., Sugawara, K., Ito, K., Takahashi, R., Ariga, H.& Mizusawa, H. :Biochem Biophys.Res. Commun, 312, 1342-1348, 2003
  11. Niki, T., Takahashi-Niki, K., Taira, T., Iguchi-Ariga, S. M. & Ariga, H.: Mol. Cancer Res. 1, 247-261, 2003,
  12. Takahashi, K., Taira, T., Niki, T., Seino, C., lguchi-Ariga, S. M. & Ariga, H.: J. Biol. Chern. 276, 37556-37563, 2001
  13. Hod, Y., Pentyala, S.N., Whyard, T.C.& El-Maghrabi, M.R.: J. Cell Biochem.72, 435-444, 1999
  14. Shendelman, S., Jcmason, A., Manimag, C., Leete, T. & Abeliovich, A.: PLoS Biol.2, e362, 2004
  15. F. Le Naour, D.E. Misek, M. C. Krause, L. Deneux, T. J. Giordano, S. Scholl and S.M. Hanash: Clin. Cancer Res. 7, 3328-3335, 2001