CircuLex Human LOXL2 ELISA Kit

  • Target LOXL2
  • Species Reactivities Human
  • Code # CY-8100
  • Size 96 Assays
  • Price
    $595.00
Specifications

Alternative Names

Lysyl oxidase-like S100

Background

Lysyl oxidase-like 2 (LOXL2) is a member of the lysyl oxidase (LOX) protein family that consists of five members (1). All the members of the family contain a highly conserved carboxy-terminal copper-binding domain and a lysyl-tyrosyl quinone cofactor, which are necessary for the amine oxidase activity of these extracellular matrix enzymes (1, 2), and the N-terminal more divergent scavenger receptor cysteine-rich domain, which is thought to determine the individual role and tissue distribution of each isoenzyme (3). In addition to its biological role in proper elastic fiber homeostasis and cardiovascular system development (4, 5), the LOX family proteins has recently been implicated in tumorigenesis and metastasis (6, 7). Up-regulation of LOXL2 has been reported in breast, colon (8), esophageal (8), head- and-neck (9), and oral squamous cell carcinomas (10) and pancreatic cancer (11), although its down-regulation of LOXL2 mRNA has been reported in head and neck squamous cell carcinomas (12). It was also reported that LOXL2 overexpression promotes the invasiveness of tumor cells in vivo and in vitro (13, 14). In addition, LOXL2 protein was detected in the serum of 83% of patients with chronic HCV infection, but was not detected in serum from normal healthy donors. This result indicates that LOXL2 is a promising candidate biomarker for the non-invasive assessment of liver fibrosis.
  • Components:
    • Microplate
    • 10X Wash Buffer
    • Dilution Buffer
    • Human LOXL2 Standard
    • HRP conjugated Detection Antibody
    • Substrate Reagent
    • Stop Solution
  • Description:
    This kit is used for the quantitative measurement of human LOXL2 in cell culture supernatant and other biological media.
  • Product Type:
    Kit
  • Sensitivity:
    better than 0.286 ng/mL of sample.
  • Short Description:
    This kit is used for the quantitative measurement of human LOXL2.
  • Size:
    96 Assays
  • Species Reactivity:
    Human
  • Storage Temperature:
    4°C
  • Target:
    LOXL2
References
  1. Csiszar,K. (2001) Lysyl oxidases: a novel multifunctional amine oxidase family. Prog. Nucleic Acid Res. Mol. Biol., 70: 1–32.
  2. Wang SX, et al. (1996) A crosslinked co-factor in lysyl oxidase: redox function for amino acid side chains. Science 273: 1078–84.
  3. Molnar,J. et al. (2003) Structural and functional diversity of lysyl oxidase and the LOX-like proteins. Biochim. Biophys. Acta, 1647: 220–24.
  4. Maki JM, Rasanen J, Tikkanen H, et al. (2002) Inactivation of the lysyl oxidase gene Lox leads to aortic aneurysms, cardiovascular dysfunction, and perinatal death in mice. Circulation 106: 2503–9.
  5. Liu X, Zhao Y, Gao J, et al. (2004) Elastic fiber homeostasis requires lysyl oxidase-like 1 protein. Nat Genet 36: 178–82.
  6. Erler JT, Bennewith KL, Nicolau M, et al. (2006) Lysyl oxidase is essential for hypoxia-induced metastasis. Nature 440: 1222–6.
  7. Payne SL, Hendrix MJ, Kirschmann DA. (2007) Paradoxical roles for lysyl oxidases in cancer- a prospect. J Cell Biochem 101: 1338–54.
  8. Fong SF, Dietzsch E, Fong KS, et al. (2007) Lysyl oxidase-like 2 expression is increased in colon and esophageal tumors and associated with less differentiated colon tumors. Genes Chromosomes Cancer 46: 644–55.
  9. Chung CH, Parker JS, Karaca G, et al. (2004) Molecular classification of head and neck squamous cell carcinomas using patterns of gene expression. Cancer Cell 5: 489–500.
  10. Ziober AF, Patel KR, Alawi F, et al. (2006) Identification of a gene signature for rapid screening of oral squamous cell carcinoma. Clin Cancer Res 12: 5960–71.
    Gronborg M, Kristiansen TZ, Iwahori A, et al. (2006) Biomarker discovery from pancreatic cancer secretome using a differential proteomic approach. Mol Cell Proteomics 5: 157–71.
  11. Rost T, Pyritz V, Rathcke IO, Gorogh T, Dunne AA, Werner JA. (2003) Reduction of LOX- and LOXL2-mRNA expression in head and neck squamous cell carcinomas. Anticancer Res 23: 1565–73.
  12. Akiri G, Sabo E, Dafni H, et al. (2003) Lysyl oxidase-related protein-1 promotes tumor fibrosis and tumor progression in vivo. Cancer Res 63: 1657–66.
  13. Kirschmann DA, Seftor EA, Fong SF, et al. (2002) A molecular role for lysyl oxidase in breast cancer invasion. Cancer Res. 62: 4478–83.