CircuLex Human Clusterin/Apo-J ELISA Kit

CircuLex HumanClusterin/Apo-J ELISA Kit.



This Kit is used for the quantitative measurement of human Clusterin/Apo-J in serum, plasma, cell culture supernatant and other biological media It can be used for 96 Assays.

Target: Clusteripo-J
Product Type: ELISA Kit
Size: 96 Assays
Application: ELISA
Research Area / Disease: Metabolism
Species Reactivity: Human
Sensitivity: better than 40.0 pg/mL of sample.
Measurement Range: 62.5 pg/mL to 4,000 pg/mL
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.


  • Microplate
  • 10X Wash Buffer
  • Dilution Buffer
  • Human Clusterin/Apo-J Standard
  • HRP conjugated Detection Antibody
  • Substrate Reagent
  • Stop Solution


  1. Jenne, D. E., and Tschopp, J. (1989) Molecular structure and functional characterization of a human complement cytolysis inhibitor found in blood and seminal plasma: identity to sulfated glycoprotein 2, a constituent of rat testis fluid. Proc. Natl. Acad. Sci. USA. 86, 7123 –7127
  2. Danik, M., Chabot, G., Mercier, C., Benabid, A. L., Chauvin, C., Quirion, R., and Suh, M. (1991) Human gliomas and epileptic foci express high levels of a mRNA related to rat testicular sulfated glycoprotein 2, a purported marker of cell death. Proc. Natl. Acad. Sci. USA. 88, 8577 –8581
  3. Palmer, D. J., and Christie, D. L. (1992) Identification of molecular aggregates containing glycoproteins III, J, K (carboxypeptidase H), and H (Kex2-related proteases) in the soluble and membrane fractions of adrenal medullary chromaffin granules. J. Biol. Chem. 267, 19806 –19812
  4. Bartl, M. M., Luckenbach, T., Bergner, O., Ullrich, O., and Koch-Brandt, C. (2001) Multiple receptors mediate apoJ-dependent clearance of cellular debris into nonprofessional phagocytes. Exp. Cell Res. 271, 130 –141
  5. Rosenberg, M. E., and J. Silkensen. 1995. Clusterin and the kidney. Exp. Nephrol. 3:9-14.
  6. Silkensen, J. R., G. B. Schwochau, and M. E. Rosenberg. 1994. The role of clusterin in tissue injury. Biochem. Cell Biol. 72:483-488
  7. Miyake, H., Gleave, M. E., Arakawa, S., Kamidono, S., and Hara, I. (2002) Introducing the clusterin gene into human renal cell carcinoma cells enhances their metastatic potential. J. Urol. 167, 2203–2208
  8. Redondo, M., Villar, E., Torres-Muñoz, J., Tellez, T., Morell, M., and Petito, C. K. (2000) Overexpression of clusterin in human breast carcinoma. Am. J. Pathol. 157, 393 –399
  9. Xie, D., Lau, S. H., Sham, J. S. T., Wu, Q. L., Fang, Y., Liang, L. Z., Che, L. H., Zeng, Y. X., and Guan, X. Y. (2005) Up-regulated expression of cytoplasmic clusterin in human ovarian carcinoma. Cancer 103, 277 –283
  10. Wellmann, A., Thieblemont, C., Pittaluga, S., Sakai, A., Jaffe, E. S., Siebert, P., and Raffeld, M. (2000) Detection of differentially expressed genes in lymphomas using cDNA arrays: identification of clusterin as a new diagnostic marker for anaplastic large-cell lymphomas. Blood 96, 398 –404
  11. Busam, K. J., Zhao, H., Coit, D. G., Kucukgol, D., Jungbluth, A. A., Nobrega, J., and Viale, A. (2005) Distinction of desmoplastic melanoma from non-desmoplastic melanoma by gene expression profiling. J. Investig. Dermatol. 124, 412 –418
  12. Miyake, H., Gleave, M., Kamidono, S., and Hara, I. (2002) Overexpression of clusterin in transitional cell carcinoma of the bladder is related to disease progression and recurrence. Urology 59, 150 –154
  13. Xie, M. J., Motoo, Y., Su, S. B., Mouri, H., Ohtsubo, K., Matsubara, F., and Sawabu, N. (2002) Expression of clusterin in human pancreatic cancer. Pancreas 25, 234 –238
  14. Kang, Y. K., Hong, S. W., Lee, H., and Kim, W. H. (2004) Overexpression of clusterin in human hepatocellular carcinoma. Hum. Pathol. 35, 1340 –1346
  15. Zhang, L. Y., Ying, W. T., Mao, Y. S., He, H. Z., Liu, Y., Wang, H. X., Liu, F., Wang, K., Zhang, D. C., Wang, Y., Wu, M., Qian, X. H., and Zhao, X. H. (2003) Loss of clusterin both in serum and tissue correlates with the tumorigenesis of esophageal squamous cell carcinoma via proteomics approaches. World J. Gastroenterol. 9, 650 –654
  16. Behrens, P., Jeske, W., Wernert, N., and Wellmann, A. (2001) Downregulation of clusterin expression in testicular germ cell tumours. Pathobiology 69, 19 –23
  17. Scaltriti, M., Brausi, M., Amorosi, A., Caporali, A., D’Arca, D., Astancolle, S., Corti, A., and Bettuzzi, S. (2004) Clusterin (SGP-2, ApoJ) expression is downregulated in low- and high-grade human prostate cancer. Int. J. Cancer 108, 23 –30
  18. Jenne, D. E., and J. Tschopp. 1989. Molecular structure and functional characterization of a human complement cytolysis inhibitor found in blood and seminal plasma: identity to sulfated glycoprotein 2, a constituent of rat testis fluid. Proc. Natl. Acad. Sci. USA 86:7123-7127
  19. Murphy, B. F., L. Kirszbaum, I. D. Walker, and A. J. F. d’Apice. 1988. SP-40,40 a newly identified normal human serum protein found in the SC5b-9 complex of complement and in the immune deposits in glomerulonephritis. J. Clin. Investig. 81:1858-1864
  20. Kirszbaum, L., S. E. Bozas, and I. D. Walker. 1992. SP-40,40, a protein involved in the control of the complement pathway, possesses a unique array of disulphide bridges. FEBS Lett. 297:70-76
  21. Humphreys, D. T., J. A. Carver, S. B. Easterbrook-Smith, and M. R. Wilson. 1999. Clusterin has chaperone-like activity similar to that of small heat shock proteins. J. Biol. Chem. 274:6875-6881
Code CY-8099
Product Type ELISA Kit
Size 96 Assays
Application ELISA

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