CircuLex Bovine Lactoferrin ELISA Kit

  • Applications
    • ELISA
  • Code # CY-8098
  • Size 96 Assays
  • Price
    $593.74
Specifications

Background

Lactoferrin is an iron-binding glycoprotein of the transferrin family that is expressed in most biologic fluids and is a major component of mammals' innate immune system (1). It is a glycoprotein consisting of a single polypeptide chain of about 80 kDa with two globular lobes each containing an iron-binding site. It is found in most exocrine secretions including milk, particularly the colostrums, and other secretions, such as tears, saliva, intestinal mucus and genital secretions, and in the specific granules of neutrophils. The physiological roles that have been proposed for lactoferrin include anti-inflammatory, immunomodulatory, antimicrobial, antiviral and antitumoral functions. For this reason, lactoferrin is regarded as a host-defense mediator. Specific lactoferrin receptors exist in a variety of cells, including monocytes, lymphocytes, adipocytes, hepatocytes, and endothelial cells (2). Therefore determination of the lactoferrin concentration in various body fluids can be a marker of inflammation (3-6). It was reported that fecal lactoferrin is useful as a sensitive and specific marker in identifying intestinal inflammation such as Crohn’s disease and chronic inflammatory bowel disease (IBD) in human (7). Combination of several markers, such as S100A8/A9 complex, defensin, elastase, MPO and I-FABP may be useful for classifying IBD. Dietary supplements of bovine lactoferrin are purported in consumer literature to enhance and support the immune system response through their antioxidant, antibacterial, and antiviral properties. Several scientific researches revealed that oral supplements of bovine lactoferrin may be a useful adjunct toward modulation of immune activity, in particular T-cell activation and antioxidant status (8).
  • Application:
    ELISA
  • Components:
    • Microplate
    • 10X Wash Buffer
    • Dilution Buffer
    • Bovine Lactoferrin Standard
    • HRP conjugated Detection Antibody
    • Substrate Reagent
    • Stop Solution
  • Description:

    The CycLex Research Product CircuLex Bovine Lactoferrin ELISA Kit is used for the quantitative measurement of bovine lactoferrin in biological samples and milk products.

  • Product Type:
    ELISA Kit
  • Research Area:
    Immunology
  • Short Description:

    CircuLex Bovine Lactoferrin ELISA Kit.

  • Size:
    96 Assays
Citations
  1. van de Looij Y et al. Lactoferrin during lactation protects the immature hypoxic-ischemic rat brain. Ann Clin Transl Neurol. 1, 955-67 (2014)
References
  1. Legrand D, Pierce A, Elass E, Carpentier M, Mariller C, Mazurier J.; Lactoferrin structure and functions. Adv. Exp. Med. Biol. 606: 163-194, 2008.
  2. Ward PP, Paz E, Conneely OM.; Multifunctional roles of lactoferrin: a critical overview. Cell Mol Life Sci. 62: 2540–8, 2005.
  3. Martins CA, Fonteles MG, Barrett LJ, Guerrant RL.; Correlation of lactoferrin with neutrophilic inflammation in body fluids. Clin Diagn Lab Immunol. 2: 763-5, 1995
  4. Rado T A, Bollekens J, St Laurent G, Parker L, Benz E J Jr.; Lactoferrin biosynthesis during granulocytopoiesis. Blood 64: 1103-9, 1984
  5. Guerrant RL, Araujo V, Soares E, Kotloff K, Lima AA, Cooper WH, Lee AG.; Measurement of fecal lactoferrin as a marker of fecal leukocytes. J Clin Microbiol. 30: 1238-42, 1992
  6. Parsi MA, Saadeh SN, Zein NN, Davis GL, Lopez R, Boone J, et al.; Ascitic fluid lactoferrin for diagnosis of spontaneous bacterial peritonitis. Gastroenterology 135: 803-7, 2008
  7. Buderus S, Boone J, Lyerly D, Lentze MJ.; Fecal lactoferrin: a new parameter to monitor infliximab therapy. Dig Dis Sci. 49: 1036-9, 2004
  8. Mulder AM, Connellan PA, Oliver CJ, Morris CA, Stevenson LM.; Bovine lactoferrin supplementation supports immune and antioxidant status in healthy human males. Nutr Res. 28: 583-9, 2008