CircuLex Mouse UCHL1 ELISA Kit

  • Applications
    • ELISA
  • Code # CY-8093
  • Size 96 Assays
  • Price
    $593.74
Specifications

Background

The UCHL1, also called neuronal-specific protein gene product 9.5, is a carboxyl-terminal ubiquitin hydrolase regulating ubiquitin dependent signaling pathways, recently suggested as a tumor suppressor. UCHL1 is expressed predominantly in neurons (1), representing 1 to 2% of total soluble brain protein (2), as well as in testis and ovary. In vivo, UCHL1 has been shown to be involved in the regulation of the ubiquitin pool, apoptosis, learning and memory, and its absence in mice because of spontaneous intragenic deletions yields phenotypes with neurological defects (3). Mutations in UCHL1 have been discovered in a German family with Parkinson disease (PD); a point mutation near the active site that changes Ile 93 to Met (I93M), which caused a partial loss of the catalytic activity of this thiol protease. This mutation has been linked to an increased risk of developing an autosomal-dominant form of PD (4). Based on the abundance in the CNS, UCHL1 has been proposed as a candidate biomarker for brain injury and ischemic strokes. It was demonstrated that UCHL1 was released from injured neurons and flow into the cerebrospinal fluid and eventually into circulating blood (5).
  • Application:
    ELISA
  • Components:
    • Microplate
    • 10X Wash Buffer
    • Dilution Buffer
    • Mouse UCHL1 Standard
    • HRP conjugated Detection Antibody
    • Substrate Reagent
    • Stop Solution
  • Description:

    The CycLex Research Product CircuLex Mouse UCHL1 ELISA Kit is used for the quantitative measurement of mouse UCHL1 in cell lysate, cell culture supernatant and other biological media.

  • Product Type:
    ELISA Kit
  • Research Area:
    Neuroscience
  • Short Description:

    CircuLex Mouse UCHL1 ELISA Kit.

  • Size:
    96 Assays
References
  1. Osaka H, Wang YL, et al. (2003) Ubiquitin carboxy-terminal hydrolase L1 binds to and stabilizes monoubiquitin in neuron. Hum Mol Genet 12: 1945-58.
  2. Wilkinson, K. D., Lee, K. M., Deshpande, S., Duerksen-Hughes, P., Boss, J. M., Pohl, J. (1989) The neuron-specific protein PGP 9.5 is a ubiquitin carboxyl-terminal hydrolase. Science 246: 670-672,
  3. Saigoh K, Wang YL, et al. (1999) Intragenic deletion in the gene encoding ubiquitin carboxy-terminal hydrolase in gad mice. Nat Genet 23: 47-51.
  4. Leroy, E., Boyer, R. et al. (1998) The ubiquitin pathway in Parkinson's disease. Nature 395: 451-452,.
  5. Papa L, Akinyi L et al. (2010) Ubiquitin C-terminal hydrolase is a novel biomarker in humans for severe traumatic brain injury. Crit Care Med. 38: 138-44.