CircuLex Human TXNIP ELISA Kit

  • Applications
    • ELISA
  • Code # CY-8090
  • Size 96 Assays
  • Price Call for Price
Specifications

Background

Thioredoxin-interacting protein (TXNIP), the endogenous inhibitor of thioredoxin also known as vitamin D3 up-regulated protein-1 (1, 2) or thioredoxin-binding protein-2 (3), inhibits thioredoxin antioxidative function by binding to its active-site thiols (3, 4). It was also demonstrated that TXNIP plays a crucial role for caspase-1 activation caused by high glucose treatment in murine β-cells by direct interaction with the NLRP3-inflammasome (5). TXNIP-dependent inflammasome activation appears to be specific for NLRP3, as TXNIP deficiency did not affect the activity of other inflammasomes, e.g., NLRC4 and AIM2 (5). TXNIP levels are elevated in subjects with type 2 diabetes mellitus (6), and its expression is induced by glucose-6-phosphate through an intracellular transcriptional complex of MondoA and Max-like protein X (7). TXNIP is induced by various types of cellular stress, including oxidative stress, UV irradiation, heat shock and apoptotic signaling (8), and is often suppressed in various human tumors (9, 10). Overexpression of TXNIP inhibits proliferation via cell-cycle arrest and promotes apoptosis (1, 4).
  • Application:
    ELISA
  • Components:
    • Microplate
    • 10X Wash Buffer
    • Dilution Buffer
    • Human TXNIP Standard
    • HRP conjugated Detection Antibody
    • Substrate Reagent
    • Stop Solution
  • Description:

    The CycLex Research Product CircuLex Human TXNIP ELISA Kit is used for the quantitative measurement of human TXNIP in cell lysate, culture medium and serum.

  • Product Type:
    ELISA Kit
  • Research Area:
    Metabolism
  • Short Description:

    CircuLex Human TXNIP ELISA Kit.

  • Size:
    96 Assays
References
  1. Wang, Y., De Keulenaer, G. W., and Lee, R. T. (2002) J. Biol. Chem. 277: 26496-26500
  2. Schulze, P. C., De Keulenaer, G. W., Yoshioka, J., Kassik, K. A., and Lee, R. T. (2002) Circ. Res. 91: 689-695
  3. Nishiyama, A., Matsui, M., Iwata, S., Hirota, K., Masutani, H., Nakamura, H., Takagi, Y., Sono, H., Gon, Y., and Yodoi, J. (1999) J. Biol. Chem. 274: 21645-21650
  4. Junn, E., Han, S. H., Im, J. Y., Yang, Y., Cho, E. W., Um, H. D., Kim, D. K., Lee, K. W., Han, P. L., Rhee, S. G., and Choi, I. (2000) J. Immunol. 164: 6287- 6295
  5. Zhou R, Tardivel A, Thorens B, Choi I, Tschopp J. (2010) Nat Immunol. 11: 136-140
  6. Parikh H, Carlsson E, Chutkow WA, et al. (2007) PLoS Med. 4: e158
  7. Stoltzman CA, Peterson CW, Breen KT, Muoio DM, Billin AN, Ayer DE. (2008) Proc Natl Acad Sci USA. 105: 6912-6917
  8. Fidler IJ, Radinsky R. (1996) J Natl Cancer Inst 88: 1700-1703.
  9. Nakamura H, Masutani H, Yodoi J. (2006) Semin Cancer Biol 16: 444-451.
  10. Goldberg SF, Miele ME, Hatta N, Takata M, Paquette-Straub C, Freedman LP et al. (2003) Cancer Res 63: 432-440.