CircuLex Human Lactoferrin ELISA Kit

  • Applications
    • ELISA
  • Code # CY-8089
  • Size 96 Assays
  • Price
    $593.74
Specifications

Background

Lactoferrin is an iron-binding glycoprotein of the transferrin family that is expressed in most biologic fluids and is a major component of mammals' innate immune system (1). It is a glycoprotein consisting of a single polypeptide chain of about 80 kDa with two globular lobes each containing an iron-binding site. It is found in most exocrine secretions including milk, particularly the colostrums, and other secretions, such as tears, saliva, intestinal mucus and genital secretions, and in the specific granules of neutrophils. The physiological roles that have been proposed for lactoferrin include anti-inflammatory, immunomodulatory, antimicrobial, antiviral and antitumoral functions. For this reason, lactoferrin is regarded as a host-defense mediator. Specific lactoferrin receptors exist in a variety of cells, including monocytes, lymphocytes, adipocytes, hepatocytes, and endothelial cells (2). Therefore determination of the lactoferrin concentration in various body fluids can be a marker of inflammation (3-6). It was reported that fecal lactoferrin is useful as a sensitive and specific marker in identifying intestinal inflammation such as Crohn’s disease and chronic inflammatory bowel disease (IBD) (7). Combination of several markers, such as S100A8/A9 complex, defensin, elastase, MPO and I-FABP may be useful for classifying IBD. Lactoferrin also directly interacts with modified LDL to prevent its interaction with scavenger receptors (8, 9). A region rich in basic amino acid residues near the lactoferrin N-terminus is responsible for the interaction with acetylated or oxidized LDL.
  • Application:
    ELISA
  • Components:
    • Microplate
    • 10X Wash Buffer
    • Dilution Buffer
    • Human Lactoferrin Standard
    • HRP conjugated Detection Antibody
    • Substrate Reagent
    • Stop Solution
  • Description:

    The CycLex Research Product CircuLex Human Lactoferrin ELISA Kit is used for the quantitative measurement of human lactoferrin in serum, plasma and other biological media.

  • Product Type:
    ELISA Kit
  • Research Area:
    Immunology
  • Short Description:

    CircuLex Human Lactoferrin ELISA Kit.

  • Size:
    96 Assays
References
  1. Legrand D, Pierce A, Elass E, Carpentier M, Mariller C, Mazurier J. ; Lactoferrin structure and functions. Adv. Exp. Med. Biol. 606: 163-194, 2008.
  2. Ward PP, Paz E, Conneely OM.; Multifunctional roles of lactoferrin: a critical overview. Cell Mol Life Sci. 62: 2540–8, 2005.
  3. Martins CA, Fonteles MG, Barrett LJ, Guerrant RL. Correlation of lactoferrin with neutrophilic inflammation in body fluids. Clin Diagn Lab Immunol. 2: 763-5, 1995
  4. Rado T A, Bollekens J, St Laurent G, Parker L, Benz E J Jr. Lactoferrin biosynthesis during granulocytopoiesis. Blood 64: 1103-9, 1984
  5. Guerrant RL, Araujo V, Soares E, Kotloff K, Lima AA, Cooper WH, Lee AG. Measurement of fecal lactoferrin as a marker of fecal leukocytes. J Clin Microbiol. 30: 1238-42, 1992
  6. Parsi MA, Saadeh SN, Zein NN, Davis GL, Lopez R, Boone J, et al. Ascitic fluid lactoferrin for diagnosis of spontaneous bacterial peritonitis. Gastroenterology 135: 803-7, 2008
  7. Buderus S, Boone J, Lyerly D, Lentze MJ.; Fecal lactoferrin: a new parameter to monitor infliximab therapy. Dig Dis Sci. 49: 1036-9, 2004
  8. Kajikawa M, Ohta T, Takase M, Kawase K, Shimamura S, Matsuda I. Lactoferrin inhibits cholesterol accumulation in macrophages mediated by acetylated or oxidized low-density lipoproteins. Biochim Biophys Acta 1213: 82–90, 1994
  9. Suginohara Y, Miyazaki A, Hakamata H, Sakamoto Y, Ohta T, Matsuda I, Horiuchi S. The heparin-bound fraction of human lipoprotein-deficient serum inhibits endocytic uptake of oxidized low density lipoprotein by macrophages. Atherosclerosis 120: 167–79, 1996