CircuLex Human YKL-39 ELISA Kit

  • Applications
    • ELISA
  • Code # CY-8087
  • Size 96 Assays
  • Price
    $593.74
Specifications

Background

YKL-39, also known as chitinase 3-like 2, is secreted chitinase-like protein which contains Glyco_18 domain with no chitinase catalytic activity but with putative lectin properties (1, 2). In contrast to its closest homologue YKL-40, YKL-39 is not a glycoprotein. YKL-39 is secreted by articular chondrocytes, also synoviocytes (3) and activates signal-regulated kinases ERK1/ERK2 in human embryonic kidney cells (4). Increased levels of YKL-39 have been demonstrated in synovial fluids of patients with osteoarthritis (OA) or rheumatoid arthritis (RA) (5, 6) as well as in malignant tumors, particularly in glioblastomas (7). It is currently recognized as a biomarker for the activation of chondrocytes and the progress of the osteoarthritis in human (5). In addition, autoantibodies to YKL-39 were detected in 8–11.8% of patients with RA (8, 9), and 11.1% of patients with OA (9), while only 1% of patients with RA had autoantibodies to YKL-40 (8). Maturation of monocyte derived macrophages in the presence of Th2 cytokine IL-4 and TGF-beta leads to the strong activation of YKL-39 expression. Thus elevated levels of YKL-39 observed during chronic inflammations can not be attributed solely to the activity of chondrocytes. In perspective, YKL-39 is also proposed as a useful biomarker to detect macrophage-specific response in tumor, atherosclerosis and neurodegenerative diseases including Alzheimer disease, in addition to the chondrocyte-specific activation.
  • Application:
    ELISA
  • Components:
    • Microplate
    • 10X Wash Buffer
    • Dilution Buffer
    • Human YKL39 Standard
    • HRP conjugated Detection Antibody
    • Substrate Reagent
    • Stop Solution
  • Description:

    The CycLex Research Product CircuLex Human YKL-39 ELISA Kit is used for the quantitative measurement of human YKL-39 in serum, plasma, cell culture medium and other biological media.

  • Product Type:
    ELISA Kit
  • Research Area:
    Cancer
  • Short Description:

    CircuLex Human YKL-39 ELISA Kit.

  • Size:
    96 Assays
References
  1. Funkhouser, J.D. and Aronson, NN. 2007. Chitinase family GH18: evolutionary insights from the genomic history of a diverse protein family. BMC Evol. Biol., 7: 96.
  2. Kzhyshkowska, J., Gratchev, A. and Goerdt, S. 2007. Human Chitinases and Chitinase-Like Proteins as Indicators for Inflammation and Cancer. Biomarker Insights, 2: 128.
  3. Hu, B. Trinh, K. Figueira, WF. Price, PA. 1996. Isolation and sequence of a novel human chondrocyte protein to mammalian members of the chitinase protein family. J Biol Chem., 271: 19415.
  4. Areshkov, PA. and Kavsan, VM. 2010. Chitinase 3-like protein 2 (CHI3L2, YKL-39) activates phosphorylation of extracellular signal-regulated kinases ERK1/ERK2 in human embryonic kidney (HEK293) and human glioblastoma (U87 MG) cells. Tsitol Genet., 44: 3.
  5. Knorr, T. Obermayr, F. Bartnik, E. Zien, A. Aigner, T. 2003. YKL-39 (chitinase 3-like protein 2), but not YKL-40 (chitinase 3-like protein 1), is up regulated in osteoarthritic chondrocytes. Ann Rheum Dis., 62: 995.
  6. Steck, E. Breit, S. Breusch, S.J. et al. 2002. Enhanced expression of the human chitinase 3-like 2 gene (YKL-39) but not chitinase 3-like 1 gene (YKL-40) in osteoarthritic cartilage. Biochem. Biophys. Res. Commun., 299: 109.
  7. Kavsan, V. Dmitrenko, V. Boyko, O. et al. 2008. Overexpression of YKL-39 Gene in Glial Brain Tumors. Scholarly Research Exchange, 2008, Article ID: 814849, doi:10.3814/2008/814849.
  8. Sekine, T. Masuko-Hongo, K. Matsui, T. et al. 2001. Recognition of YKL-39, a human cartilage related protein, as a target antigen in patients with rheumatoid arthritis. Ann. Rheum. Dis., 60: 49.
  9. Tsuruha, J. Masuko-Hongo, K. Kato, T. et al. 2002. Autoimmunity against YKL-39, a human cartilage derived protein, in patients with osteoarthritis. J. Rheumatol., 29: 1459.