CircuLex S100A4 ELISA Kit Ver.2

  • Applications
    • ELISA
  • Code # CY-8086
  • Size 96 Assays
  • Price


Members of the S100 protein family are low molecular mass acidic proteins characterized by cell-type-specific expression and the presence of 2 EF-hand calcium-binding domains with different affinities for calcium (1). Elevated levels of one S100 protein, S100A4, are closely associated with the process of metastasis in breast and other cancer cells in rodent animal models and in human cancer specimens. S100A4 or its mRNA is found at an elevated level in metastatic relative to non-metastatic rat (2) and mouse (3) tumor cell lines and malignant relative to benign human breast tumors (4). Elevation of the level of rat (5) or human (6) S100A4 in benign rat mammary tumor cells results in the acquisition of metastatic capability by some of the cells. In transgenic mouse models of breast cancer, elevated levels of S100A4 in neu oncogene-induced (7), or in murine mammary tumor virus-induced (8), benign mammary tumors yield lung metastases. In colorectal adenocarcinoma specimens, elevated levels of immunocytochemically detected S100A4 are associated with the more malignant carcinomatous regions of the primary tumors and with liver metastases (9).
  • Application:
  • Components:
    • Microplate
    • 10X Wash Buffer
    • Dilution Buffer
    • S100A4 Standard
    • HRP conjugated Detection Antibody
    • Substrate Reagent
    • Stop Solution
  • Description:

    The CycLex Research Product Circulex S100A4 ELISA Kit is used for the quantitative measurement of human S100A4 in cell extract, tissue culture medium and other biological media.

  • Product Type:
    ELISA Kit
  • Research Area:
  • Short Description:

    CircuLex S100A4 ELISA Kit Ver. 2.

  • Size:
    96 Assays
  1. Šenolt L et al. High levels of metastasis-inducing S100A4 protein and treatment outcome in early rheumatoid arthritis: data from the PERAC cohort. Biomarkers. 20, 47-51 (2015),
  2. Abu El-Asrar AM et al. S100A4 is upregulated in proliferative diabetic retinopathy and correlates with markers of angiogenesis and fibrogenesis. Mol Vis. 20, 1209-24 (2014) ,
  3. Pleštilová L et al. The metastasis promoting protein S100A4 levels associate with disease activity rather than cancer development in patients with idiopathic inflammatory myopathies. Arthritis Res Ther. 16, 468 (2014)
  4. Turnier JL et al. Urine S100 proteins as potential biomarkers of lupus nephritis activity. Arthritis Res Ther. 19, 242 (2017)
  5. Boukais K et al.Clearance of plasmin-PN-1 complexes by vascular smooth muscle cells in human aneurysm of the ascending aorta. Cardiovasc Pathol. 32, 15-25 (2018)
  1. Hilt, D., and Kligman, D. (1991) in Novel Calcium-binding Proteins: Fundamentals and Clinical Implications (Heizmann, C. W., ed) , pp. 65-103, Springer-Verlag, Berlin
  2. Dunnington, D. J. (1984) The Development and Study of Single Cell-cloned Metastasizing Mammary Tumour Cell Systems in the Rat. Ph.D. thesis , University of London
  3. Ebralidze, A., Tulchinsky, E., Grigorian, M., Afanayeva, A., Senin, V., Revazova, E., and Lukanidin, E. (1989) Genes Dev. 3, 1086-1093
  4. Nikitenko, L., Lloyd, B., Rudland, P., Fear, S., and Barraclough, R. (2000) Int. J. Cancer 86, in press
  5. Davies, B., Davies, M., Gibbs, F., Barraclough, R., and Rudland, P. (1993) Oncogene 8, 999-1008
  6. Lloyd, B., Platt-Higgins, A., Rudland, P., and Barraclough, R. (1998) Oncogene 17, 465-473
  7. Davies, M., Rudland, P., Robertson, L., Parry, E., Jolicoeur, P., and Barraclough, R. (1996) Oncogene 13, 1631-1637
  8. Ambartsumian, N., Grigorian, M., Larsen, F., Karlstrom, O., Sidenius, N., Rygaard, J., Georgiev, G., and Lukanidin, E. (1996) Oncogene 13, 1621-1630
  9. Takenaga, K., Nakanishi, H., Wada, K., Suzuki, M., Matsuzaki, O., Matsuura, A., and Endo, H. (1997) Clin. Cancer Res. 3, 2309-2316