CircuLex 14-3-3 γ ELISA Kit

  • Applications
    • ELISA
  • Code # CY-8082
  • Size 96 Assays
  • Price
    $593.74
Specifications

Background

14-3-3 proteins are a family of conserved regulatory molecules expressed in all eukaryotic cells. There are seven genes, β, γ, ε, σ, ζ, τ and η, that encode 14-3-3s in most mammals. 14-3-3 proteins have the ability to bind phospho-Ser/Thr motifs, as well as phosphorylation-independent interactions, on a multitude of functionally diverse signaling proteins, including kinases, phosphatases, and transmembrane receptors. The family dynamically regulates the activity of target proteins in various signaling pathways that control diverse physiological and pathological processes. More than 100 signaling proteins have been reported as 14-3-3 proteins’ targets. Either an abnormal state of 14-3-3 protein expression or dysregulation of 14-3-3/terget protein interactions contributes to the development of many human diseases. Clinical investigations have demonstrated a correlation between up-regulated 14-3-3 protein levels and poor survival of cancer patients. Several studies have also suggested that 14-3-3 isoforms are differentially regulated in cancer and neurological syndromes. Especially, elevated amounts of the gamma isoform (14-3-3 Gamma) are found in the cerebrospinal fluid (CSF) of Creutzfeldt-Jakob disease (CJD) patients and could be as a specific marker of the disease as well as tau protein level.
  • Application:
    ELISA
  • Components:
    • Microplate
    • 10X Wash Buffer
    • Sample/Standard Dilution Buffer
    • 14-3-3 Gamma Standard
    • 00X 14-3-3 Gamma Detection Antibody
    • Detection Antibody Dilution Buffer
    • HRP conjugated Anti-IgG Antibody
    • Conjugate Dilution Buffer
    • Substrate Reagent
    • Stop Solution
  • Description:

    The CycLex Research Product CircuLex 14-3-3 Gamma ELISA Kit is used for the quantitative measurement of 14-3-3 γ (gamma isoform protein) in CSF (Cerebrospinal fluid), cell lysates and other biological samples in units of concentration. It can be used for 96 Assays.

  • Product Type:
    ELISA Kit
  • Research Area:
    Cell Biology
  • Short Description:

    CircuLex 14-3-3 γ ELISA Kit.

  • Size:
    96 Assays
Citations
  1. Cramm M et al. Characteristic CSF prion seeding efficiency in humans with prion diseases. Mol Neurobiol. 51, 396-405 (2015)
References
  1. The 14-3-3 brain protein in cerebrospinal fluid as a marker for transmissible spongiform encephalopathies. Hsich G, Kenney K, Gibbs CJ, Lee KH, Harrington MG. (1996) N Engl J Med. 335(13):924-30.
  2. Isoform pattern of 14-3-3 proteins in the cerebrospinal fluid of patients with Creutzfeldt-Jakob disease. Wiltfang J, Otto M, Baxter HC, Bodemer M, Steinacker P, Bahn E, Zerr I, Kornhuber J, Kretzschmar HA, Poser S, Rüther E, Aitken A. (1999) J Neurochem. 73(6): 2485-90.
  3. An enzyme-linked immunosorbent assay to quantify 14-3-3 proteins in the cerebrospinal fluid of suspected Creutzfeldt-Jakob disease patients. Kenney K, Brechtel C, Takahashi H, Kurohara K, Anderson P, Gibbs CJ Jr. (2000) Ann Neurol. 48(3):395-8.
  4. Determination of 14-3-3 protein levels in cerebrospinal fluid from Creutzfeldt-Jakob patients by a highly sensitive capture assay. Peoc'h K, Schröder HC, Laplanche J, Ramljak S, Müller WE. (2001) Neurosci Lett. 301(3):167-70.
  5. CSF tests in the differential diagnosis of Creutzfeldt-Jakob disease. Sanchez-Juan P, Green A, Ladogana A, Cuadrado-Corrales N, Sáanchez-Valle R, Mitrováa E, Stoeck K, Sklaviadis T, Kulczycki J, Hess K, Bodemer M, Slivarichová D, Saiz A, Calero M, Ingrosso L, Knight R, Janssens AC, van Duijn CM, Zerr I. (2006) Neurology. 67(4):637-43.
  6. Total tau protein in cerebrospinal fluid and diffusion-weighted MRI as an early diagnostic marker for Creutzfeldt-Jakob disease. Satoh K, Shirabe S, Tsujino A, Eguchi H, Motomura M, Honda H, Tomita I, Satoh A, Tsujihata M, Matsuo H, Nakagawa M, Eguchi K. (2007) Dement Geriatr Cogn Disord. 24(3):207-12. (Epub 2007 Aug 10.)
  7. The role of cerebrospinal fluid proteins as early diagnostic markers for sporadic Creutzfeldt-Jakob disease. Pennington C, Chohan G, Mackenzie J, Andrews M, Will R, Knight R, Green A. (2009)Neurosci Lett. 455(1):56-9. (Epub 2009 Mar 5.)
  8. High sensitivity of an ELISA kit for detection of the gamma-isoform of 14-3-3 proteins: usefulness in laboratory diagnosis of human prion disease. Matsui Y, Satoh K, Miyazaki T, Shirabe S, Atarashi R, Mutsukura K, Satoh A, Kataoka Y, Nishida N. (2011) BMC Neurol. 11: 120.