Products Research Area/Disease Autophagy Reagents CircuLex Human AIM/CD5L/Spα　ELISA kit

CircuLex Human AIM/CD5L/Spα　ELISA kit

CircuLex Human AIM/CD5L/Spα ELISA Kit.

Specifications:

Description

The CycLex Research Product CircuLex Human AIM/CD5L/Spα ELISA Kit is used for the quantitative measurement of free form human AIM/CD5L/Spα in serum. It can be used for 96 Assays.

Target:	CD5L
Product Type:	ELISA Kit
Size:	96 Assays
Application:	ELISA
Research Area / Disease:	Immunology
Species Reactivity:	Human
Sensitivity:	The limit of detection (defined as such a concentration of human AIM/CD5L/Spα giving absorbance higher than mean absorbance of blank* plus three standard deviations of the absorbance of blank: A blank + 3SD blank) is better than 0.745 ng/ml of sample.
Measurement Range:	The measurement range is 1.57 ng/mL to 100 ng/mL. Any sample reading higher than the highest standard should be diluted with Dilution Buffer in higher dilution and re-assayed. Dilution factors need to be taken into consideration in calculating the human AIM/CD5L/Spα concentration.
Regulatory Statement:	For Research Use Only. Not for use in diagnostic procedures.

Components

Microplate
10X Wash Buffer
Dilution Buffer
Human AIM/CD5L/Spα Standard
20x HRP conjugated Detection Antibody
Conjugate Dilution Buffer
Substrate Reagent
Stop Solution

Citations

Mera K et al. Serum levels of apoptosis inhibitor of macrophage are associated with hepatic fibrosis in patients with chronic hepatitis C. BMC Gastroenterol. 14, 27 (2014),
Xu DD et al. Discovery and identification of serum potential biomarkers for pulmonary tuberculosis using iTRAQ-coupled two-dimensional LC-MS/MS. Proteomics. 14, 322-31 (2014)

References

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Maria-Rosa Sarrias, Sandra Roselló, Fernando Sánchez-Barbero, Josep M. Sierra, Jordi Vila, José Yélamos, Jordi Vives, Cristina Casals, and Francisco Lozano J. Biol. Chem. 280: 35391-35398, 2005
Tissot, J. D., Sanchez, J. C., Vuadens, F., Scherl, A., Schifferli, J. A., Hochstrasser, D. F., Schneider, P., and Duchosal, M. A. Electrophoresis 23: 1203–1206, 2002
Arai, S.; Shelton, J. M.; Chen, M.; Bradley, M. N.; Castrillo, A.; Bookout, A. L.; Mak, P. A.; Edwards, P. A.; Mangelsdorf, D. J.; Tontonoz, P.; Miyazaki, T. Cell Metab. 1: 201-213, 2005.
WK Kim, HR Hwang, do H Kim, PY Lee, YJ In, HY Ryu, SG Park, KH Bae, and SC Lee. Exp Mol Med 40: 677-685, 2008
Wu J, Kobayashi M, Sousa EA, Liu W, Cai J, Goldman SJ, Dorner AJ, Projan SJ, Kavuru MS, Qiu Y, Thomassen MJ. Mol Cell Proteomics 4: 1251-64, 2005
Gangadharan B, Antrobus R, Dwek RA, Zitzmann N. Novel serum biomarker candidates for liver fibrosis in hepatitis C patients. Clin Chem 53: 1792-1799, 2007
Joe Gray et al. BMC Cancer 9: 271, 2009

CircuLex Human AIM/CD5L/Spα ELISA kit