CircuLex Human AIM/CD5L/Spα ELISA kit

  • Applications
    • ELISA
  • Code # CY-8080
  • Size 96 Assays
  • Price
    $593.74
Specifications

Background

AIM, also called CD5L/SPα, is a soluble 38 or 40-kDa glycoprotein expressed by macrophages present in lymphoid tissues (spleen, lymph node, thymus, and bone marrow), for which little functional and structural information is available (1). It belongs to the group B of the scavenger receptor cysteine-rich (SRCR) superfamily that includes the lymphocyte surface receptors CD5 and CD6 among others. AIM is able to bind to not only myelomonocytic and lymphoid cells (2), but also the surface of several Gram-positive and Gram-negative bacterial strains (3), which suggests that it may play an important role in the regulation of the innate and adaptive immune systems. It has been reported that AIM is present in IgM fraction, but not IgG or IgA fractions, from human serum (4). AIM is a circulating protein that may play a role in the homeostasis of IgM antibodies. In addition, it has been reported that AIM expression is induced in mouse macrophages in response to loading with highly oxidized low density lipoprotein (5), and that AIM is expressed in foam cells within atherosclerotic lesions. AIM may be feasible as a potential biomarker in the diagnosis and treatment of atopic dermatitis (6), although AIM was reported as candidate marker in asthma (7) and liver cirrhosis (8, 9)
  • Application:
    ELISA
  • Components:
    • Microplate
    • 10X Wash Buffer
    • Dilution Buffer
    • Human AIM/CD5L/Sp alpha; Standard
    • 20x HRP conjugated Detection Antibody
    • Conjugate Dilution Buffer
    • Substrate Reagent
    • Stop Solution
  • Description:

    The CycLex Research Product CircuLex Human AIM/CD5L/Spα ELISA Kit is used for the quantitative measurement of free form human AIM/CD5L/Spα in serum, plasma and other biological media. It can be used for 96 Assays.

  • Product Type:
    ELISA Kit
  • Research Area:
    Autophagy
  • Short Description:

    CircuLex Human AIM/CD5L/Spα ELISA Kit.

  • Size:
    96 Assays
Citations
  1. Mera K et al. Serum levels of apoptosis inhibitor of macrophage are associated with hepatic fibrosis in patients with chronic hepatitis C. BMC Gastroenterol. 14, 27 (2014),
  2. Xu DD et al. Discovery and identification of serum potential biomarkers for pulmonary tuberculosis using iTRAQ-coupled two-dimensional LC-MS/MS. Proteomics. 14, 322-31 (2014)
References
  1. Gebe, J. A.; Kiener, P. A.; Ring, H. Z.; Li, X.; Francke, U.; Aruffo, A. J. Biol. Chem. 272: 6151-6158, 1997.
  2. Maria-Rosa Sarrias, Sandra Roselló, Fernando Sánchez-Barbero, Josep M. Sierra, Jordi Vila, José Yélamos, Jordi Vives, Cristina Casals, and Francisco Lozano J. Biol. Chem. 280: 35391-35398, 2005
  3. Tissot, J. D., Sanchez, J. C., Vuadens, F., Scherl, A., Schifferli, J. A., Hochstrasser, D. F., Schneider, P., and Duchosal, M. A. Electrophoresis 23: 1203–1206, 2002
  4. Arai, S.; Shelton, J. M.; Chen, M.; Bradley, M. N.; Castrillo, A.; Bookout, A. L.; Mak, P. A.; Edwards, P. A.; Mangelsdorf, D. J.; Tontonoz, P.; Miyazaki, T. Cell Metab. 1: 201-213, 2005.
  5. WK Kim, HR Hwang, do H Kim, PY Lee, YJ In, HY Ryu, SG Park, KH Bae, and SC Lee. Exp Mol Med 40: 677-685, 2008
  6. Wu J, Kobayashi M, Sousa EA, Liu W, Cai J, Goldman SJ, Dorner AJ, Projan SJ, Kavuru MS, Qiu Y, Thomassen MJ. Mol Cell Proteomics 4: 1251-64, 2005
  7. Gangadharan B, Antrobus R, Dwek RA, Zitzmann N. Novel serum biomarker candidates for liver fibrosis in hepatitis C patients. Clin Chem 53: 1792-1799, 2007
  8. Joe Gray et al. BMC Cancer 9: 271, 2009