CircuLex Human Chitotriosidase ELISA Kit

CircuLex Human Chitotriosidase ELISA Kit.



The CycLex Research Product CircuLex Human Chitotriosidase ELISA Kit is used for the quantitative measurement of human chitotriosidase in human serum/plasma, cell extract, cell conditioned medium and other biological samples. It can be used for 96 Assays.

Target: CHITO
Product Type: ELISA Kit
Size: 96 Assays
Application: ELISA
Research Area / Disease: Cell Biology, Neuroscience
Species Reactivity: Human



  • Microplate
  • 10xWash Buffer
  • Dilution Buffer
  • Human Chitotriosidase Standard
  • HRP conjugated Detection Antibody
  • Substrate Reagent
  • Stop Solution


  1. Sanlialp, Sara Cetin, Gokay Nar, and Hande Senol. “Clinical significance of chitotriosidase in outpatients with advanced heart failure.” Journal of Investigative Medicine 69.3 (2021): 736-74.
  2. Gray, Elizabeth, et al. “CSF chitinases before and after symptom onset in amyotrophic lateral sclerosis.” Annals of clinical and translational neurology 7.8 (2020): 1296-1306.
  3. Fujisawa, Tomoyuki, et al. “Clinical significance of serum chitotriosidase level in Anti-MDA5 antibody–positive dermatomyositis-associated interstitial lung disease.” The Journal of rheumatology 46.8 (2019): 935-942.
  4. Thompson, Alexander G., et al. “CSF chitinase proteins in amyotrophic lateral sclerosis.” Journal of Neurology, Neurosurgery & Psychiatry 90.11 (2019): 1215-1220.
  5. Varghese AM et al. Chitotriosidase – a putative biomarker for sporadic amyotrophic lateral sclerosis. Clin Proteomics. 10, 19 (2013)


  1. Hollak, C. E., van Weely, S., van Oers, M. H. and Aerts, J. M. (1994). Marked elevation of plasma chitotriosidase activity. A novel hallmark of Gaucher disease. J. Clin. Invest. 93: 1288.
  2. Renkema, G. H., Boot, R. G., Muijsers,A.O., Donker-Koopman, W. E. and Aerts, J. M. (1995). Purification and characterization of human chitotriosidase, a novel member of the chitinase family of proteins. J. Biol. Chem. 270: 2198.
  3. Boot, R. G., Renkema, G. H., Strijland, A., van Zonneveld, A. J. and Aerts, J. M. (1995). Cloning of a cDNA encoding chitotriosidase, a human chitinase produced by macrophages. J. Biol. Chem. 270: 26252.
  4. Hakala BE, White C, Recklies AD. (1993) Human cartilage gp-39, a major secretory product of articular chondrocytes and synovial cells, is a mammalian member of chitinase protein family. J Biol Chem. 268: 25803.
  5. Renkema GH, Boot RG, Au FL, Donker-Koopman WE, Strijland A, Muijsers AO, Hrebicek M, Aerts JM. (1998) Chitotriosidase, a chitinase, and the 39-kDa human cartilage glycoprotein, a chitin-binding lectin, are homologues of family 18 glycosyl hydrolases secreted by human macrophages. Eur J Biochem. 251: 504.
  6. Aerts, J. M., and Hollak, C. E. (1997) Plasma and metabolic abnormalities in Gaucher’s disease. Baillieres Clin. Haematol. 10: 691.
  7. Guo Y, He W, Boer AM, Wevers RA, de Bruijn AM, Groener JE, Hollak CE, Aerts JM, Galjaard H, van Diggelen OP. (1995) Elevated plasma chitotriosidase activity in various lysosomal storage disorders. J Inher Metab Dis. 18: 717.
  8. Vedder, A.C., Cox-Brinkman, J., Hollak, C.E. et al. (2006) Plasma chitotriosidase in male Fabry patients: A marker for monitoring lipid-laden macrophages and their correction by enzyme replacement therapy. Mol. Genet. Metab. 89: 239.
  9. Grosso, S., Margollicci, M.A., Bargagli, E. et al. (2004) Serum levels of chitotriosidase as a marker of disease activity and clinical stage in sarcoidosis. Scand. J. Clin. Lab. Invest.. 64: 57.
  10. Boot, R.G., van Achterberg, T.A., van Aken, B.E. et al. (1999) Strong induction of members of the chitinase family of proteins in atherosclerosis: chitotriosidase and human cartilage gp-39 expressed in lesion macrophages. Arterioscler. Thromb. Vasc. Biol. 19: 687.
  11. Artieda, M., Cenarro, A., Ganan, A. et al. (2003) Serum chitotriosidase activity is increased in subjects with atherosclerosis disease. Arterioscler. Thromb. Vasc. Biol. 23: 1645.
  12. Labadaridis, J., Dimitriou, E., Costalos, C. et al. (1998) Serial chitotriosidase activity estimations in neonatal systemic candidiasis. Acta Paediatr. 87: 605.
  13. Labadaridis, I., Dimitriou, E., Theodorakis, M. et al. (2005) Chitotriosidase in neonates with fungal and bacterial infections. Arch. Dis. Child Fetal Neonatal Ed. 90: F531. 14. Barone, R., Simpore, J., Malaguarnera, L., Pignatelli, S. And Musumeci, S. (2003) Plasma chitotriosidase activity in acute Plasmodium falciparum malaria. Clin. Chim. Acta 331: 79.
  14. Di Rosa, M., Musumeci, M., Scuto, A., Musumeci, S. and Malaguarnera, L. (2005) Effect of interferon-gamma, interleukin-10, lipopolysaccharide and tumor necrosis factor-alpha on chitotriosidase synthesis in human macrophages. Clin. Chem. Lab. Med. 43: 499.
  15. Malaguarnera, L.,Musumeci,M., Licata, F., Di Rosa,M.,Messina, A. and Musumeci, S. (2004) Prolactin induces chitotriosidase gene expression in human monocyte-derived macrophages. Immunol. Lett. 94: 57.
  16. Renkema, G. H., Boot, R. G., Strijland, A. Donker-koopman, W. E., van den Berg, M., Muijsers, A. O., and Aerts, J. M. F. G. (1997) Synthesis, sorting, and processing into distinct isoforms of human macrophage chitotriosidase. Eur. J. Biochem. 244: 279
  17. Eiberg H, den Tandt WR. (1997) Assignment of human plasma methylumbelliferyl -tetra-N-acetylchitotetraoside or chitinase to chromosome 19 by a linkage study. Hum Genet. 101: 205.