CircuLex Rat NGAL/Lipocalin-2 ELISA Kit

CircuLex Rat NGAL/Lipocalin-2 ELISA Kit.



The CycLex Research Product CircuLex Rat NGAL/Lipocalin-2 ELISA Kit is used for the quantitative measurement of rat NGAL/Lipocalin-2 in serum samples. It can be used for 96 Assays.

Target: NGAL/Lipocalin-2
Product Type: ELISA Kit
Size: 96 Assays
Application: ELISA
Research Area / Disease: Metabolism
Sensitivity: The limit of detection (defined as such a concentration of rat NGAL giving absorbance higher than mean absorbance of blank* plus three standard deviations of the absorbance of blank: A blank + 3SD blank) is better than 6.02 pg/ml of sample.
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.


  • Microplate
  • 10X Wash Buffer
  • Dilution Buffer
  • Rat NGAL Standard
  • Biotinylated Detection Antibody
  • HRP conjugated Streptavidin
  • Substrate Reagent
  • Stop Solution


  1. Giasson J, Li GH, Chen Y.; Neutrophil gelatinase-associated lipocalin (NGAL) as a new biomarker for non–acute kidney injury (AKI) diseases. Inflamm Allergy Drug Targets. 10: 272-82, 2011


  1. Kjeldsen L, Johnsen AH, Sengelov H, Borregaard N. 1993; Isolation and primary structure of NGAL, a novel protein associated with human neutrophil gelatinase. J Biol Chem. 268: 10425-32.
  2. Devireddy, L.R., Teodoro, J.G., Richard, F.A. and Green, M.R., 2001; Induction of apoptosis by a secreted lipocalin that is transcriptionally regulated by IL-3 deprivation. Science 293: 829–834.
  3. Yang, J., Goetz, D., Li, J.-Y., Wang, W., Mori, K., Setlik, D., Du, T., Erdjument-Bromage, H., Tempst, P., Strong, R. and Barasch, J., 2002; Iron delivery pathway mediated by a lipocalin. Mol. Cell 10: 1045–1056.
  4. Bundgaard, J.R., Sengelov, H., Borregaard, N. and Kjeldsen, L., 1994; Molecular cloning and expression of a cDNA encoding NGAL: a lipocalin expressed in human neutrophils. BBRC. 202: 1468–1475.
  5. Chu, S.T., Lin, H.J. and Chen, Y.H., 1997. Complex formation between a formyl peptide and 24p3 protein with a blocked N-terminus of pyroglutamate. J. Pept. Res. 49: 582–585. 6. Sengelov, H., Boulay, F., Kjeldsen, L. and Borregaard, N., 1994. Subcellular localization and translocation of the receptor for N-formylmethionyl-leucyl-phenylalanine in human neutrophils. Biochem. J. 299: 473–479.
  6. Xu SY, Pauksen K, Venge P. 1995: Serum measurements of human neutrophil lipocalin (HNL) discriminate between acute bacterial and viral infections. Scand J Clin Lab Invest 55: 125-131.
  7. Matthaeus T, Schulze-Lohoff E, Ichimura T, Weber M, Andreucci M, Park KM, Alessandrini A, Bonventre JV. 2001; Co-regulation of neutrophil gelatinase-associated lipocalin and matrix metalloproteinase-9 in the postischemic rat kidney. J Am Soc Nephrol 12: 787A.
  8. Ohlsson S, Wieslander J, Segelmark M. 2003; Increased circulating levels of proteinase 3 in patients with anti-neutrophilic cytoplasmic autoantibodies-associated systemic vasculitis in remission. Clin Exp Immunol 131: 528-535.
  9. Mishra J, Ma Q, Prada A, Mitsnefes M, Zahedi K, Yang J, Barasch J, Devarajan P. 2003); Identification of neutrophil gelatinase-associated lipocalin as a novel early urinary biomarker for ischemic renal injury. J Am Soc Nephrol 14: 2534-2543.
  10. Amin RP, Vickers AE, Sistare F, Thompson KL, Roman RJ, Lawton M, Kramer J, Hamadeh HK, Collins J, Grissom S, Bennett L, Tucker CJ, Wild S, Kind C, Oreffo V, Davis JW 2nd, Curtiss S, Naciff JM, Cunningham M, Tennant R, Stevens J, Car B, Bertram TA, Afshari CA. 2004; Identification of putative gene based markers of renal toxicity. Environ Health Perspect 112: 465-479.
  11. Mishra J, Mori K, Ma Q, Kelly C, Barasch J, Devarajan P. 2004; Neutrophil gelatinase-associated lipocalin: a novel early urinary biomarker for cisplatin nephrotoxicity. Am J Nephrol 24: 307-315.
  12. Mishra J, Dent C, Tarabishi R, Mitsnefes MM, Ma Q, Kelly C, Ruff SM, Zahedi K, Shao M, Bean J, Mori K, Barasch J, Devarajan P. 2005; Neutrophil gelatinase-associated lipocalin (NGAL) as a biomarker for acute renal injury after cardiac surgery. Lancet 365: 1231-1238.
Code CY-8053
Product Type ELISA Kit
Size 96 Assays
Application ELISA

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