CircuLex Mouse Adiponectin ELISA Kit

  • Applications
    • ELISA
  • Code # CY-8051
  • Size 96 Assays
  • Price
    $497.49
Specifications

Background

Adiponectin, also referred as Acrp30, AdipoQ and GBP-28, is a recently discovered 244 aminoacid protein, the product of the apM1 gene, which is physiologically active and specifically and highly expressed in adipose cells (adipokine). The protein belongs to the soluble defence collagen superfamily; it has a collagen-like domain structurally homologous with collagen VIII and X and complement factor C1q-like globular domain (1, 2). Adiponectin forms homotrimers, which are the building blocks for higher order complexes found circulating in serum (3, 4). Circulating Adiponectin levels are high (5-30 g/mL), accounting for approximately 0.01% of total plasma protein (5-8). Adiponectin receptors AdipoR1 and AdipoR2 have been recently cloned; AdipoR1 is abundantly expressed in skeletal muscle, whereas AdipoR2 is predominantly expressed in the liver (9). Paradoxically, adipose tissue-expressed adiponectin levels are inversely related to the degree of adiposity (10,11). A reduction in adiponectin serum levels is accompanied by insulin resistance states, such as obesity and type 2 diabetes mellitus (12, 13). It is also reported in patients with coronary artery disease (13). Increased adiponectin levels are associated with type 1 diabetes mellitus, anorexia nervosa and chronic renal failure. Adiponectin concentrations correlate negatively with glucose, insulin, triglyceride concentrations and body mass index and positively with high-density lipoprotein-cholesterol levels and insulin-stimulated glucose disposal. Adiponectin has been shown to increase insulin sensitivity and decrease plasma glucose by increasing tissue fat oxidation. It inhibits the inflammatory processes of atherosclerosis suppressing the expression of adhesion and cytokine molecules in vascular endothelial cells and macrophages, respectively. This adipokine plays a role as a scaffold of newly formed collagen in myocardial remodelling after ischaemic injury and also stimulates angiogenesis by promoting cross-talk between AMP-activated protein kinase and Akt signalling in endothelial cells (14). Injection of Adiponectin into non-obese diabetic mice leads to an insulin-independent decrease in glucose levels (15). This is likely due to insulin-sensitizing effects involving Adiponectin-regulation of triglyceride metabolism (15). A truncated form of Adiponectin (gAdiponectin) containing only the C-terminal globular domain has been identified in the blood, and recombinant gAdiponectin has been shown to regulate weight reduction as well as free fatty acid oxidation in mouse muscle and liver (16, 17). The full-length recombinant Adiponectin protein is apparently less potent at mediating these effects (16, 17).
  • Application:
    ELISA
  • Components:
    • Microplate
    • 10X Wash Buffer
    • Dilution Buffer
    • Mouse Adiponectin Standard
    • HRP conjugated Detection Antibody
    • Substrate Reagent, Stop Solution
  • Description:

    The CircuLex Mouse Adiponectin ELISA Kit is used for the quantitative measurement of mouse or rat adiponectin in serum, plasma, tissue culture medium and other biological media It can be used for 96 Assays.

  • Product Type:
    ELISA Kit
  • Research Area:
    Metabolism
  • Short Description:

    CircuLex Mouse Adiponectin ELISA Kit.

  • Size:
    96 Assays
Citations
  1. Chan Hum Park, Jeong Sook Noh, Noriko Yamabe, Ki Sung Kang, Takashi Tanaka and Takako Yokozawa; Beneficial effect of 7-O-galloyl-d-sedoheptulose on oxidative stress and hepatic and renal changes in type 2 diabetic db/db mice. European Journal of Pharmacology, 640: 233-42, 2010,
  2. Hiroyuki Kameji, Kazuki Mochizuki, Noriyuki Miyoshi and Toshinao Goda; β-Carotene accumulation in 3T3-L1 adipocytes inhibits the elevation of reactive oxygen species and the suppression of genes related to insulin sensitivity induced by tumor necrosis factor-α. Nutrition 26: 1151-1156, 2010,
  3. Kanda Y et al. Dietary restriction preserves the mass and function of pancreatic β cells via cell kinetic regulation and suppression of oxidative/ER stress in diabetic mice. J Nutr Biochem. 26, 219-26 (2015),
  4. Kanda Y, Shimoda M, Hamamoto S, Tawaramoto K, Kawasaki F, Hashiramoto M, Nakashima K, Matsuki M, Kaku K.; Molecular mechanism by which pioglitazone preserves pancreatic β cells in obese diabetic mice: Evidence for acute and chronic actions as a PPAR agonist. Am J Physiol Endocrinol Metab, 298: E278-E286, 2010,
  5. Noh JS, Park CH, Kim HY, Zhao Q, Yamabe N, Matsumoto K, Yokozawa T.; Chinese prescription Kangen-karyu prevents dyslipidaemia and oxidative stress in mouse model of type 2 diabetes. J Pharm Pharmacol. 63: 111-9.2011,
  6. Noriko Yamabe, Jeong Sook Noh, Chan Hum Park, Ki Sung Kang, Naotoshi Shibahara, Takashi Tanaka and Takako Yokozawa; Evaluation of loganin, iridoid glycoside from Corni Fructus, on hepatic and renal glucolipotoxicity and inflammation in type 2 diabetic db/db mice. European Journal of Pharmacology 648: 179-187, 2010,
  7. Park CH et al. Evaluation of 7-O-galloyl-D-sedoheptulose, isolated from Corni Fructus, in the adipose tissue of type 2 diabetic db/db mice. 89, 131-42 (2013),
  8. Park MH et al. Potent anti-diabetic effects of MHY908, a newly synthesized PPAR α/β dual agonist in db/db mice. PLoS One. 8, e78815 (2013),
  9. Saki Fujimoto, Kazuki Mochizuki, Masaya Shimada, Tomoyo Hori, Yuki Murayama, Norio Ohashi and Toshinao God; Insulin resistance induced by a high-fat diet is associated with the induction of genes related to leukocyte activation in rat peripheral leukocytes. Life Sciences, 87: 679-85, 2010,
  10. Tominaga S, Nishi K, Nishimoto S, Akiyama K, Yamauchi S, Sugahara T.; (-)-Secoisolariciresinol attenuates high-fat diet-induced obesity in C57BL/6 mice. Food Funct. 3: 76-82. 2012
  11. Kameji H et al. β-Carotene accumulation in 3T3-L1 adipocytes inhibits the elevation of reactive oxygen species and the suppression of genes related to insulin sensitivity induced by tumor necrosis factor-α. Nutrition 26, 1151-6 (2010)
  12. Fujimoto S et al. Insulin resistance induced by a high-fat diet is associated with the induction of genes related to leukocyte activation in rat peripheral leukocytes. Life Sci. 87, 679-85 (2010)
  13. Yamabe N et al. Evaluation of loganin, iridoid glycoside from Corni Fructus, on hepatic and renal glucolipotoxicity and inflammation in type 2 diabetic db/db mice. Eur J Pharmacol. 648, 179-187 (2010)
  14. Sakurai N et al. BRD4 regulates adiponectin gene induction by recruiting the P-TEFb complex to the transcribed region of the gene. Sci Rep. 7, 11962 (2017)
References
  1. Maeda, K. et al. (1996) Biochem. Biophys. Res. Commun. 221:286.
  2. Kishore, U. and K.B. Reid (2000) Immunopharmacology 49:159.
  3. Shapiro, L. and P.E. Scherer (1998) Curr. Biol. 8:335.
  4. Nakano, Y. et al. (1996) J. Biochem. (Tokyo) 120:803.
  5. Scherer, P.E. et al. (1995) J. Biol. Chem. 270:26746.
  6. Fruebis, J. et al. (2001) Proc. Natl. Acad. Sci. USA 98:2005.
  7. Berg, A.H. et al. (2002) Trends Endocrinol. Metab. 13:84.
  8. Arita, Y. et al. (1999) Biochem. Biophys. Res. Commun. 257:79.
  9. Yamauchi, T. et al. (2003) Nature 423:762.
  10. Stefan, N. et al. (2002) J. Clin. Endocrinol. Metab. 87:4652.
  11. Matsubara, M. et al. (2002) Eur. J. Endocrinol. 147:173.
  12. Weyer, C. et al. (2001) J. Clin. Endocrinol. Metab. 86:1930.
  13. Hotta, K. et al. (2000) Arterioscler. Thromb. Vasc. Biol. 20:1595.
  14. Tomas, E. et al. (2002) Proc. Natl. Acad. Sci. USA 99:16309.
  15. Berg, A.H. et al. (2001) Nat. Med. 7:947.
  16. Fruebis, J. et al. (2001) Proc. Natl. Acad. Sci. USA 98:2005.
  17. Yamauchi, T. et al. (2001) Nat. Med. 7:941.