CycLex® Poly-Ubiquitinated Protein ELISA Kit

  • Applications
    • ELISA
  • Code # CY-7053
  • Size 96 Assays
  • Price
    $593.74
Specifications

Background

The ubiquitin-proteasome pathway is the principle pathway of proteolysis in eukaryotic cells and may contribute to controlling the intracellular levels of a variety of short-lived proteins (1-3), in addition to degrading abnormal proteins in the cytosol and nucleus. Protein substrates are marked with a poly-ubiquitin chain (4) and then degraded to peptides and free ubiquitin by a large multicatalytic complex, the proteasome, which exists within all eukaryotic cells (1-3). Numerous examples of regulatory proteins have been found to undergo ubiquitin-dependent proteolysis. Protein substrates of the ubiquitin-proteasome pathway include a number of cell regulatory molecules, such as cyclins, the Myc oncogene protein, and p53, and the regulated degradation of these molecules has been linked to the control of cell proliferation and cell cycle progression (5-7). By controlling the intracellular levels of such proteins, the activity of the ubiquitin-proteasome pathway might also be linked to apoptosis. In a recent decade, a variety of reversible (8) and irreversible (11) inhibitors of the 20 S proteasome have been identified that can enter mammalian cells and inhibit degradation of proteins by the ubiquitin-proteasome pathway. One group of such inhibitors are peptide aldehydes, e.g. MG132 which reversibly bind to active sites and inhibit cleavage of hydrophobic or acidic substrates (8). A more specific inhibitor is the naturally occurring bacterial compound, lactacystin, which covalently modifies threonine residues in the proteasome's active site and does not seem to affect any other known protease (11). Such agents can inhibit protein degradation (8-11) and major histocompatibility class I antigen presentation (8) in a variety of mammalian cells and have been widely used to probe the physiological function of the ubiquitin-proteasome pathway.
  • Application:
    ELISA
  • Components:
    • Microplate
    • 10X Wash Buffer
    • Cell Extraction Buffer
    • Dilution Buffer
    • Poly-Ubiquitinated Protein Standard
    • HRP conjugated Detection Antibody
    • Conjugate Dilution Buffer
    • Substrate Reagent
    • Stop Solution
  • Description:

    The CycLex Research Product Poly-Ubiquitinated Protein ELISA Kit is designed to detect and
    quantify the level of total poly-ubiquitinated proteins in cell lysate. It can be used for 96 Assays.

  • Product Type:
    ELISA Kit
  • Research Area:
    Autophagy
  • Short Description:

    CycLex Poly-Ubiquitinated Protein ELISA Kit.

  • Size:
    96 Assays
Citations
  1. Oberbach A et al. A global proteome approach in uric acid stimulated human aortic endothelial cells revealed regulation of multiple major cellular pathways. Int J Cardiol. 176, 746-52 (2014)
References
  1. Goldberg A. L., Stein R., Adams J. Chem. Biol., 2: 503-508, 1995.
  2. Coux O., Tanaka K., Goldberg A. L. Annu. Rev. Biochem., 65: 801-847, 1996.
  3. King R. W., Deshaies R. J., Peters J-M., Kirschner M. W. Science, 274: 1652-1659, 1996.
  4. Chau V., Tobias J. W., Bachmair A., Marriott D., Ecker D. J., Gonda D. K., Varshavsky A. Science, 243: 1576-1583, 1989.
  5. Hochstrasser, M. Curr. Opin. Cell Biol. 7: 215-223, 1995
  6. Ciechanover, A. Cell 79: 13-21, 1994
  7. Jentsch, S., and Schlenker, S. Cell 82: 881-884, 1995
  8. Rock, K. L., Gramm, C., Rothstein, L., Clark, K., Stein, R., Dick, L., Hwang, D., and Goldberg, A. L. Cell 78: 761-771, 1994
  9. Jensen, T. J., Loo, M. A., Pind, S., Williams, D. B., Goldberg, A. L., and Riordan, J. R. Cell 83: 129-135, 1994
  10. Ward, C. L., Omura, S., and Kopito, R. R. Cell 83: 121-127, 1995
  11. Fenteany, G., Standaert, R. F., Lane, W. S., Choi, S., Corey, E. J., and Schreiber, S. L. Science 268: 726-731, 1995