CycLex® Phospho-p53 Ser46 ELISA Kit

  • Applications
    • ELISA
  • Code # CY-7050
  • Size 96 Assays
  • Price
    $593.74
Specifications

Background

p53 is a short-lived, non-abundant protein that regulates the response of cells to DNA damage, in part through transcriptional activation of genes involved in cell cycle control, DNA repair, and apoptosis (1). p53 is a tumor suppressor protein; consequently, mice in which the p53 gene has been disrupted develop tumors with high frequency (2, 3), and deletions or mutations in the p53 gene are prevalent in a majority of human cancers (4, 5). The protein level and activity of p53 are regulated, and this is accomplished by MDM2 (murine double minute 2) (6–10). The MDM2 gene is induced by p53, and MDM2 prevents apoptosis by inhibiting p53 activity and promoting its degradation (6, 9, 11). In response to DNA damage, the p53 protein is phosphorylated on each of the seven serines and one threonine the in the first 50 amino acids of its N-terminal transactivation domain as well as at several sites in its C-terminal tetramerization/regulatory domain (12, 13). As a transcription factor, p53 induces or represses several genes that regulate cell cycle arrest, DNA repair or apoptosis, including p21WAF1, MDM2, GADD45, p53R2, and p53AIP1. Recent studies suggest that specific p53 phosphorylation events are important for the activation or repression of specific promoters (14-17). It has been shown that the proline-rich domain (residues 64-92) and a transcriptional activation domain (residues 43-63) of p53 have each been suggested to be necessary for mediation of apoptosis, because deletion of either of these two domains abolishes this activity. It was found that phosphorylation of Ser46 is induced after DNA damage in vivo. Particularly, phosphorylation of Ser46 was observed only at the late stage after DNA damage by several different agents compared to Ser15 or Ser20. Moreover, upon severe DNA damage by UV, Ser46 on p53 is phosphorylated and apoptosis is induced. In addition, substitution of Ser46 to Alanine inhibits the ability of p53 to induce apoptosis but not G1 arrest. These results suggested that phosphorylation of Ser46 is involved in induction of p53-dependent apoptosis.
  • Application:
    ELISA
  • Components:
    • Microplate
    • 10X Wash Buffer
    • Cell Extraction Buffer
    • Dilution Buffer
    • p53 S46 Standard
    • Stop Solution
    • Primary Antibody Solution (Anti-Phospho-p53 Monoclonal Antibody TK-4D4)
    • Secondary Antibody Solution (HRP conjugated Anti-Mouse IgG)
    • Substrate Reagent 
  • Description:

    The CycLex Research Product Phospho-p53 S46 ELISA Kit is designed to detect and quantify the level of human p53 protein that is phosphorylated at serine residue 46 It can be used for 96 Assays.

  • Product Type:
    ELISA Kit
  • Sensitivity:
    better than 1.450 units/ml of sample.
  • Short Description:

    CycLex Phospho-p53 Ser46 ELISA Kit.

  • Size:
    96 Assays