CycLex® Protein Phosphatase DUSP1/MKP-1 Fluorometric Assay Kit

  • Target DUSP1/MKP-1
  • Code # CY-1373
  • Size 40 Assays
  • Price
    $540.75
Specifications

Background

DUSP1 is a member of a family of dual-specificity phosphatases that dephosphorylate both phosphothreonine and phosphotyrosine residues and contain a highly conserved C-terminal catalytic domain and an N-terminal Cdc25-like (CH2) domain. DUSP1 is also known as MKP-1 (mitogenactivated protein (MAP) kinase phosphatase-1) and inactivates MAP kinase by the concomitant dephosphorylation of both its phosphothreonine and phosphotyrosine residues. The expression of DUSP1/MKP-1 is induced in human skin fibroblasts by oxidative/heat stress and growth factors. DUSP1/MKP-1 may play an important role in the human cellular response to environmental stress as well as in the negative regulation of cellular proliferation via the dephosphorylation of MAP kinase. A recent study* also shows that DUSP1/MKP-1 is a key factor of major depressive disorder (MDD) and it may be a new drug targets for treating depression and possibly other mood disorders. * Duric V, Banasr M, Licznerski P, Schmidt HD, Stockmeier CA, Simen AA, Newton SS, Duman RS. A negative regulator of MAP kinase causes depressive behavior. Nat Med. 16: 1328-32, 2010.
  • Components:
    • 10X DUSP1/MKP-1 Assay Buffer
    • 10X OMFP,Recombinant DUSP1/MKP-1
    • 10X Phosphatase Inhibitor
    • Stop Solution
  • Description:

    The CycLex Research product Protein Phosphatase DUSP1/MKP-1 Fluorometric Assay Kit is a fluorometric and non-radioactive assay designed to measure the activity of DUSP1/MKP-1 protein phosphatase. It can be used for 40 assays.

  • Product Type:
    Kit
  • Short Description:

    CycLex Protein Phosphatase DUSP1/MKP-1 Fluorometric Assay Kit.

  • Size:
    40 Assays
  • Storage Temperature:
    -70°C
  • Target:
    DUSP1/MKP-1
Citations
  1. Kim HJ et al. The water-soluble triptolide derivative PG490-88 protects against cisplatin-induced acute kidney injury. J Pharmacol Exp Ther. 349, 518-25 (2014)
References
  1. Alessi, D. R., Smythe, C., Keyse, S. M. The human CL100 gene encodes a tyr/thr-protein phosphatase which potently and specifically inactivates MAP kinase and suppresses its activation by oncogenic ras in Xenopus oocyte extracts. Oncogene 8: 2015-2020, 1993.
  2. Bhalla, U. S., Ram, P. T., Iyengar, R. MAP kinase phosphatase as a locus of flexibility in a mitogen-activated protein kinase signaling network. Science 297: 1018-1023, 2002.
  3. Brondello, J.-M., Pouyssegur, J., McKenzie, F. R. Reduced MAP kinase phosphatase-1 degradation after p42/p44(MAPK)-dependent phosphorylation. Science 286: 2514-2517, 1999.
  4. Keyse, S. M., Emslie, E. A. Oxidative stress and heat shock induce a human gene encoding a protein-tyrosine phosphatase. Nature 359: 644-647, 1992.
  5. Patterson, K. I., Brummer, T., O'Brien, P. M., Daly, R. J. Dual-specificity phosphatases: critical regulators with diverse cellular targets.  Biochem. J. 418: 475-489, 2009.
  6. Wang, X., Meng, X., Kuhlman, J. R., Nelin, L. D., Nicol, K. K., English, B. K., Liu, Y. Knockout of Mkp-1 enhances the host inflammatory responses to Gram-positive bacteria. J. Immun. 178: 5312-5320, 2007.
  7. Calvisi DF, Pinna F, Meloni F, Ladu S, Pellegrino R, Sini M, Daino L, Simile MM, De Miglio MR, Virdis P, Frau M, Tomasi ML, Seddaiu MA, Muroni MR, Feo F, Pascale RM. Dual-specificity phosphatase 1 ubiquitination in extracellular signal-regulated kinase-mediated control of growth in human hepatocellular carcinoma. Cancer Res. 68: 4192-200, 2008.
  8. Duric V, Banasr M, Licznerski P, Schmidt HD, Stockmeier CA, Simen AA, Newton SS, Duman RS. A negative regulator of MAP kinase causes depressive behavior. Nat Med. 16: 1328-32, 2010