CycLex® Protein Phosphatase LMW-PTP/ACP1 Fluorometric Assay Kit

CycLex Protein Phosphatase LMW-PTP/ACP1 Fluorometric Assay Kit.

Specifications:

Description

The CycLex Protein Phosphatase LMW-PTP/ACP1 Fluorometric Assay Kit is a fluorometric and non-radioactive assay designed to measure the activity of LMW-PTP/ACP1 protein phosphatase. It can be used for 100 Assays.

Target: LMW-PTP/ACP1
Product Type: Kit
Size: 100 Assays
Research Area / Disease: Cell Biology
Storage Temperature: -70°C
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.

Components

  • 10X Assay Buffer
  • 10X OMFP
  • Recombinant LMW-PTP/ACP1
  • 100X Phosphatase Inhibitor
  • Stop Solution
  • Instruction Manual

References

  1. Dissing J, Johnsen AH, Sensabaugh GF. Human red cell acid phosphatase (ACP1). The amino acid sequence of the two isozymes Bf and Bs encoded by the ACP1*B allele. J Biol Chem. 1991 Nov 5;266(31):20619-25.
  2. Wo YY, McCormack AL, Shabanowitz J, Hunt DF, Davis JP, Mitchell GL, Van Etten RL. Sequencing, cloning, and expression of human red cell-type acid phosphatase, a cytoplasmic phosphotyrosyl protein phosphatase. J Biol Chem. 1992 May 25;267(15):10856-65.
  3. Chiarugi P, Cirri P, Raugei G, Manao G, Taddei L, Ramponi G. Low M(r) phosphotyrosine protein phosphatase interacts with the PDGF receptor directly via its catalytic site. Biochem Biophys Res Commun. 1996 Feb 6;219(1):21-5.
  4. Chiarugi P, Cirri P, Marra F, Raugei G, Camici G, Manao G, Ramponi G. LMW-PTP is a negative regulator of insulin-mediated mitotic and metabolic signaling. Biochem Biophys Res Commun. 1997 Sep 18;238(2):676-82.
  5. Fiaschi T, Chiarugi P, Buricchi F, Giannoni E, Taddei ML, Talini D, Cozzi G, Zecchi-Orlandini S, Raugei G, Ramponi G. Low molecular weight protein-tyrosine phosphatase is involved in growth inhibition during cell differentiation. J Biol Chem. 2001 Dec 28;276(52):49156-63.
  6. Kikawa KD, Vidale DR, Van Etten RL, Kinch MS. Regulation of the EphA2 kinase by the low molecular weight tyrosine phosphatase induces transformation. J Biol Chem. 2002 Oct 18;277(42):39274-9.
  7. Chiarugi P, Taddei ML, Schiavone N, Papucci L, Giannoni E, Fiaschi T, Capaccioli S, Raugei G, Ramponi G. LMW-PTP is a positive regulator of tumor onset and growth. Oncogene. 2004 May 13;23(22):3905-14.
  8. Malentacchi F, Marzocchini R, Gelmini S, Orlando C, Serio M, Ramponi G, Raugei G. Up-regulated expression of low molecular weight protein tyrosine phosphatases in different human cancers. Biochem Biophys Res Commun. 2005 Sep 2;334(3):875-83.
  9. Parri M, Buricchi F, Taddei ML, Giannoni E, Raugei G, Ramponi G, Chiarugi P. EphrinA1 repulsive response is regulated by an EphA2 tyrosine phosphatase. J Biol Chem. 2005 Oct 7;280(40):34008-18.
Code CY-1358
Product Type Kit
Size 100 Assays
Price
$566.37
Qty: